ABSTRACT
A new chromene (1) and six known compounds identified as 6-hydroxymellein (2), 6-hydroxy-5-methylmellein (3) nectriapyrone (4), chermesinone A(5), chermesinone B(6), and pomopxanthone A(7), were isolated in our investigation of the cytotoxic constituents from the fermented rice substrate of endophytic fungus Phomopsis sp. HCCB03519. The structures of these com pounds were elucidated through spectroscopic data analysis. All compounds exhibited inhibitory activities against cancer cell lines. Compound 7 showed stronger inhibition against cancer cells than the positive control 5-Fu.
Subject(s)
Humans , Antineoplastic Agents , Chemistry , Pharmacology , Ascomycota , Chemistry , Benzopyrans , Chemistry , Pharmacology , Cell Line, Tumor , Fluorouracil , Chemistry , Pharmacology , Isocoumarins , Chemistry , Pharmacology , Molecular StructureABSTRACT
Two novel isocoumarins, erigeronone C (1) and D (2), were isolated from the ethanol extract of the whole plant of Erigeron breviscapus (Vant.) Hand.-Mazz (Compositae). Their structures were respectively elucidated as 8, 9-dihydroxypyrano [3, 2-c] isochromen-4, 6-dione (1) and 4, 7-dihydroxy-3-(3-hydroxy-4-oxo-4H-pyran-2-yl)-1H-isochromen-1-one (2) on the basis of spectral analyses. Both structures of 1 and 2 possess a gamma-pyrone moiety and that is rare in natural products.
Subject(s)
Erigeron , Chemistry , Isocoumarins , Chemistry , Molecular Structure , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To search for new antitumor active lead compounds from marine microorganism.</p><p><b>METHOD</b>A marine strain, Aspergillus terreus, was cultured and up-scaled in artificial seawater media, from which the metabolites were isolated and elucidated by using modern spectroscopy techniques.</p><p><b>RESULT</b>Twelve compounds were isolated from mycelia and fermentation broth of A. terreus.</p><p><b>CONCLUSION</b>Compounds 1-4 were steroids, compounds 5-8 were organic acids and esters, compound 9 was an alkaloid, compound 10 was an isocoumarin, compound 11 was ceramide, compound 12 was propenyl cyclic pentanediol.</p>
Subject(s)
Alkaloids , Chemistry , Metabolism , Aspergillus , Chemistry , Metabolism , Ceramides , Chemistry , Metabolism , Culture Media , Chemistry , Metabolism , Esters , Chemistry , Metabolism , Isocoumarins , Chemistry , Metabolism , Mycelium , Chemistry , Metabolism , Propylene Glycols , Chemistry , Metabolism , Rhizophoraceae , Microbiology , Steroids , Chemistry , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Botrychium lanuginosum.</p><p><b>METHOD</b>Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated by chemical evidence and spectroscopic methods.</p><p><b>RESULT</b>Ten compounds were isolated from the 95% ethanol extract of the herb of B. lanuginosum and their structures were elucidated as 30-nor-21beta-hopan-22-one (1), beta-sitosterol (2), luteolin (3), thunberginol A (4), apigenin (5), (6'-O-palmitoyl)-sitosterol-3-O-beta-D-glucoside (6), daucosterol (7), 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2R-hydroxy hexadecanoyl) amino]-4, 8-octadecadiene-1, 3-diol (8), luteolin-7-O-glucoside (9), sucrose (10).</p><p><b>CONCLUSION</b>Compounds 1-10 were isolated from this genus for the first time.</p>
Subject(s)
Apigenin , Chemistry , Chromatography , Drugs, Chinese Herbal , Chemistry , Ferns , Chemistry , Isocoumarins , Chemistry , Luteolin , Chemistry , Sitosterols , Chemistry , Sucrose , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To in vestigate the constituents in root of Gentiana macrophylla.</p><p><b>METHOD</b>Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, 1H-, 13C-NMR etc.) and identified by comparing with standard substance.</p><p><b>RESULT</b>Eight compounds were identified. Four compounds isolated from the chloroform fraction are: 5-carboxyl-3,4-dihydrogen-1H-2-benzopyran-1-one (1), erythrocentauric acid (2), roburic acid (3), oleanolic acid (4). Water fraction gave four known secoiridoid glucosides. They were: gentiopicroside (5), swertiamarine (6), sweroside (7), 6'-O-beta-D-glucosylgentiopicroside (8).</p><p><b>CONCLUSION</b>1 is a novel compound. It was named as erythrocentauric acid. 2 was isolated from genus Gentiana and 8 was isolated from G. macrophylla for the first time.</p>