ABSTRACT
Erectile dysfunction (ED) associated with type 2 diabetes is a severe problem that requires effective treatment. Pancreatic kininogenase (PK) has the potential to improve the erectile function of ED patients. This study aims to investigate the effect of PK on erectile function in streptozotocin-induced type 2 diabetic ED rats. To achieve this goal, we divided male Sprague-Dawley rats into five groups. One group was not treated, and the other four groups were treated with saline, sildenafil, PK or sildenafil, and PK, respectively, for 4 weeks after the induction of type 2 diabetic ED. Then, intracavernous pressure under cavernous nerve stimulation was measured, and penile tissue was collected for further study. Endothelial nitric oxide synthase levels, smooth muscle content, endothelium content, cyclic guanosine monophosphate (cGMP) levels in the corpus cavernosum, and neuronal nitric oxide synthase levels in the dorsal penile nerve were measured. Improved erectile function and endothelium and smooth muscle content in the corpus cavernosum were observed in diabetic ED rats. When treating diabetic ED rats with PK and sildenafil at the same time, a better therapeutic effect was achieved. These data demonstrate that intraperitoneal injection of PK can improve erectile function in a rat model of type 2 diabetic ED. With further research on specific mechanisms of erectile function improvement, PK may become a novel treatment for diabetic ED.
Subject(s)
Animals , Male , Rats , Cyclic GMP/metabolism , Diabetes Mellitus, Experimental/physiopathology , Erectile Dysfunction/physiopathology , Kallikreins/therapeutic use , Muscle, Smooth, Vascular/physiopathology , Nitric Oxide Synthase Type I/metabolism , Nitric Oxide Synthase Type III/metabolism , Penile Erection/physiology , Penis/metabolism , Rats, Sprague-Dawley , Sildenafil Citrate/therapeutic use , Treatment Outcome , Urological Agents/therapeutic useABSTRACT
Com o proposito de avaliar a resistencia do Plasmodium falciparum as drogas antimalaricas, rotineiramente empregadas no Brasil, os autores acompanham dez pacientes com malaria falciparum adquirida na Amazonia brasileira. Os pacientes foram submetidos a estudo in vivo de sensibilidade a drogas, apos tratamento com derivados 4-aminoquinoleinicos (cloroquina e amodiaquina) ou quinino. A absorcao das drogas foi verificada atraves de testes padronizados de excrecao urinaria de antimalaricos. Oito pacientes puderam ser seguidos por 28 dias...