[Evaluation of expression and intensity of cytiokeratin-10 and 19 in odontogenic keratocyst [OKC] with immunohistochemical staining and comparing it with dentigerous cyst and dental follicle]

Odontogenic keratocyst [OKC] and dentigerous cyst are two most common developmental odontogenic cysts involving jaws. OKC has a high growth potential in contrast to its minor clinical manifestation. It has also a high degree of recurrence rate. The purpose of this study was to diagnose the OKC and dentigerous cysts and also differentiate them based on their immunohistochemical criteria. To provide the materials for this research, we preliminarely reviewed the records of the patients referred to pathology lab of shahid Beheshti Dental College between March 1999 and March 2003 and 60 paraffin-embedded biopsies [20 OKC, 20 dentigerous cysts and 20 dental follicles] were selected. Then immunhistochemical staining was applied to identify cytokeratin-10 [CK-10] and cytokeratin-19 [CK-19] and their conditions, as well. The expression of CK-10, CK-19 are seen in OKC and dentigerous cyst. CK-10 was found more in OKC, along with CK-19 in dentigerous cyst. The expression of these cytokeratins was more in upper half of the epithelial lining of these cysts. One of the dental follicles expressed CK-19, but not CK-10. CK-10 is more specific for OKC as well as CK-19 for dentigerous cyst. These cytokeratins are usually seen in the upper layers of the cyst lining. If an expression of cytokeratin-19 is found in a dental follicle, it may correspond the cystic transformation of the latter one

Carcinosarcoma of the Liver: A Case Report

Primary hepatic carcinosarcoma is a rare tumor comprised of a mixture of carcinomatous and sarcomatous elements. Less than 20 adequately documented cases have been reported, however the imaging features of two cases were briefly described. We present here a case of carcinosarcoma of the liver in a 46-year-old woman, which was confirmed based on pathology. Imaging showed a large mass with large necrotic portions, small cystic portions, calcifications and bone formations.

Diagnostic Value of Galectin-3, HBME-1, Cytokeratin 19, High Molecular Weight Cytokeratin, Cyclin D1 and p27(kip1) in the Differential Diagnosis of Thyroid Nodules

The distinction between benign and malignant thyroid tumors is critical for the management of patients with thyroid nodules. We applied immunohistochemical staining for galectin-3, HBME-1, cytokeratin 19 (CK19), high molecular weight cytokeratin (HMWCK), cyclin D1 and p27(kip1) in 295 thyroid lesions to determine their diagnostic accuracy. The expression of all markers was significantly associated with differentiated thyroid carcinoma (DTC).The sensitivity for the diagnosis of DTC was 94.7% with galectin-3, 91.3% with HBME-1, and 90.3% with CK19. The specificities of these markers were 95.5%, 69.7%, and 83.1%, respectively. Combining these markers, co-expression of galectin-3 and CK19 or galectin-3 and HBME-1 was seen in 93.2% of carcinomas but in none of the benign nodules. Comparing follicular variant of papillary carcinoma (FVPC) with follicular carcinoma (FC), the expression of galectin-3, CK19, and HMWCK was significantly higher in FVPC. When comparing FC with FA, the expression of galectin-3 and HBME-1 was significantly higher in FC. These results suggest that 1) galectin-3 is a useful marker in the distinction between benign and malignant thyroid tumors, 2) the combined use of HBME-1 and CK19 can increase the diagnostic accuracy, and 3) the use of CK19 and HMWCK can aid in the differential diagnosis between PC and FC.