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1.
Arq. bras. oftalmol ; 82(1): 56-61, Jan.-Feb. 2019. tab, graf
Article in English | LILACS | ID: biblio-973867

ABSTRACT

ABSTRACT Purpose: The porcine eye is frequently used as a research model. This paper analyzes the effect of different storage methods on the transparency of pig crystalline lens. Methods: A spectral transmission curve (from 220 to 780 nm) for the crystalline lens was determined experimentally after storage in different conditions: saline solution, formalin, castor oil, and freezing at -80°C. The total transmission in the visible spectrum, which was used as an index of transparency, was calculated from these curves. For comparative purposes, fresh lenses were evaluated and used as controls. Results: Storing the porcine crystalline lens in saline solution or castor oil resulted in a transparency loss of approximately 10% after 24 h and storage in formalin resulted in a loss of nearly 30%. Storage by freezing at -80°C for 4 weeks maintained the transparency of the crystalline lens; the spectral transmission measured immediately after defrosting at room temperature coincided exactly with that of the freshly extracted lens. Conclusions: The transparency of porcine crystalline lens is affected by the storage method. The visible spectrum is the most affected, evidenced by the effect on the transparency and consequently the amount of light transmitted. The results show that freezing at -80°C maintains the transpa rency of the crystalline lens for at least 4 weeks.


RESUMO Objetivos: Olho de porco é frequentemente usa do como modelos de pesquisa. Este estudo analisa o efeito de di ferentes métodos de armazenamento na preservação da transparência do cristalino de porco. Métodos: Uma curva de transmissão espectral (de 220 até 780 nm) para o cristalino foi experimentalmente determinada após armazenamento em diferentes condições: solução salina, formol, óleo de mamona e congelamento a -80°C. Transmissão total do espectro visível, que foi usada como um índice de transparência foi calculada a partir dessas curvas. Para fins comparativos, lentes frescas foram avaliadas e usadas como controles. Resultados: O armazenamento do cristalino suíno em solução salina ou óleo de mamona resultou uma perda de transparência de aproximadamente 10% após 24 h e o armazenamento em formol resultou uma perda de quase 30%. O armazenamento por congelamento a -80°C durante 4 semanas manteve a transparência do cristalino; a transmissão espectral medida imediatamente após o descongelamen to à temperatura ambiente coincidiu exatamente com a da lente extraída recentemente. Conclusão: A transparência do cristalino suíno é afetada pelo método de armazenamento. O espectro visível é o mais afetado, evidenciado pelo efeito sobre a transparência e consequentemente a quantidade de luz transmitida. Os resultados mostram que o congelamento a -80°C mantém a transparência do cristalino suíno por pelo menos 4 semanas.


Subject(s)
Animals , Organ Preservation/methods , Lens, Crystalline/anatomy & histology , Reference Values , Spectrophotometry/instrumentation , Spectrophotometry/methods , Swine , Time Factors , Ultraviolet Rays , Castor Oil/chemistry , Reproducibility of Results , Models, Animal , Formaldehyde/chemistry , Freezing , Lens, Crystalline/physiology , Lens, Crystalline/diagnostic imaging , Light
2.
Rev. Soc. Colomb. Oftalmol ; 52(1): 60-61, 2019. ilus.
Article in Spanish | LILACS, COLNAL | ID: biblio-1026476

ABSTRACT

Se presenta el caso de una mujer de 81 años que acude para valoración de cirugía de catarata en el ojo derecho. El examen biomicroscópico revela la existencia de una catarata C2N4 (Lens Opacities Classifi cation System, versión III) con una cápsula anterior que en su región central pareciera estar abierta a modo de capsulorrexis, aunque sin trasvase de material lenticular ni signos inflamatorios en cámara anterior Figura 1. Su consistencia y morfología nos hacen pensar en un posible desdoblamiento idiopático de la cristaloides anterior, ya que tanto el reborde pupilar como la gonioscopia son rigurosamente normales. De ahí nuestro título de "pseudocapsulorrexis".


Subject(s)
Capsulorhexis , Cataract Extraction , Lens, Crystalline/diagnostic imaging
3.
Rev. bras. oftalmol ; 77(4): 184-188, jul.-ago. 2018. tab, graf
Article in English | LILACS | ID: biblio-959100

ABSTRACT

ABSTRACT Objective: To evaluate the correlation of optical biometry and target with variable ages, anterior chamber depth, axial length, R1/K1 and R2/K2 established by two optical biometry devices in patients with cataract. Methods: The study included the analysis of 348 medical records, from which 503 cataract eyes were selected, which underwent evaluation by the optical biometric devices IOL Master 700 and Lenstar LS 900 in the period of April to July 2017. Data collected were: age, anterior chamber depth, axial length, R1/K1 and R2/K2. Results: The average of the biometrics obtained using Lenstar was 21.02, varying 3.46, more or less, with an average target of -0.02, varying 0.45. In relation to the IOL Master it was 21.19, with a variation of 3.40 and average target of -0.01, a variation of 0.11. It can be observed that despite close values in relation to the mean, there was significance (p<0.001). Axial length (p<0.001) and R1/K1 (p<0.001) had an influence on the difference of the biometric values between the devices. Conclusion: A high degree of clinical and statistical correspondence was observed between the results obtained by the biometry devices in patients with cataract.


RESUMO Objetivos: Avaliar a correlação da biometria óptica e target com as variáveis idade, profundidade da câmara anterior, comprimento axial, R1/K1 e R2/K2 estabelecidos por dois dispositivos de biometria óptica em pacientes com catarata. Métodos: O trabalho abrangeu a análise de 348 prontuários dos quais foram selecionados 503 olhos com catarata ,que passaram pela avaliação nos dispositivos de biometria óptica IOL Master 700 e Lenstar LS 900 no período de abril a julho de 2017. Os dados colhidos foram: idade, profundidade da câmara anterior, comprimento axial, R1/K1 e R2/K2 . Resultados: A média da biometria obtida utilizando o Lenstar foi de 21,02, variando 3,46 para mais ou para menos, com target médio de -0,02, variando 0,45. Já em relação ao IOL Master foi de 21,19, com variação de 3,40 e target médio de -0,01, variação de 0,11. Pode-se observar que apesar de valores próximos em relação à média, houve significância (p < 0,001). Houve a concordância da biometria em relação ao comprimento axial (p < 0,001) e R1/K1(p < 0,001). Conclusão: Observou-se alto grau de correspondência clínica e estatística entre os resultados obtidos pelos dispositivos de biometria em pacientes com catarata.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Refractive Errors/diagnostic imaging , Cataract/diagnosis , Biometry/instrumentation , Biometry/methods , Axial Length, Eye/diagnostic imaging , Lenses, Intraocular/standards , Anterior Chamber/diagnostic imaging , Refraction, Ocular/physiology , Cataract Extraction/methods , Visual Acuity , Cross-Sectional Studies , Reproducibility of Results , Cornea/diagnostic imaging , Corneal Topography/instrumentation , Lens Implantation, Intraocular/methods , Preoperative Period , Electronic Health Records , Observational Study , Lens, Crystalline/diagnostic imaging
5.
Arq. bras. oftalmol ; 79(5): 328-329, Sept.-Oct. 2016. graf
Article in English | LILACS | ID: biblio-827975

ABSTRACT

ABSTRACT A 42-year-old woman was admitted to our clinic with a complaint of glare in both eyes. Biomicroscopic examination of both the eyes revealed iris and lens colobomas in the inferior quadrant. Fundus examination of the right eye revealed an oval and gray inferotemporal optic pit and two choroid colobomas in the inferior quadrant. In the left eye, two choroid colobomas were detected that were inferior to the optic nerve head. Furthermore, a 21-year-old man presented to our clinic for a routine ophthalmologic examination. Bilateral biomicroscopic examination was normal. Fundus examination of the left eye revealed an oval and gray inferotemporal optic pit and a choroid coloboma that was inferior to the optic nerve head. Here we describe optic pits co-occurring with iris, lens, and choroidal colobomas. On the basis of these cases, a defect in the closure of the embryonic fissure is the most plausible etiology of the optic pit.


RESUMO Uma mulher de 42 anos de idade foi internada em nossa clínica com queixa de ofuscamento em ambos os olhos. O exame biomicroscópico revelou coloboma de íris e cristalino no quadrante inferior em ambos os olhos. O exame de fundo do olho direito revelou um fosseta óptica oval e acinzentada na região inferotemporal e dois colobomas coroide no quadrante inferior. No olho esquerdo, dois colobomas de coroide foram detectados inferiormente à da cabeça do nervo óptico. Outro homem de 21 anos apresentou-se em nossa clínica para um exame oftalmológico de rotina. O exame biomicroscópico foi normal, bilateralmente. O exame de fundo do olho esquerdo revelou uma fosseta oval e acinzentada de nervo óptico óptico inferotemporal e um coloboma coroide inferior à cabeça do nervo óptico. Nestes relatos nós descrevemos fossetas ópticas ocorrendo simultaneamente com colobomas de íris, cristalino, e coroide. Com base nestes casos, o defeito no fechamento da fissura embrionária é uma provável etiologia da fosseta óptica.


Subject(s)
Humans , Male , Female , Adult , Young Adult , Optic Disk/abnormalities , Coloboma/pathology , Iris/abnormalities , Choroid/abnormalities , Lens, Crystalline/abnormalities , Optic Disk/pathology , Optic Disk/diagnostic imaging , Fluorescein Angiography/methods , Visual Acuity , Coloboma/diagnostic imaging , Iris/pathology , Iris/diagnostic imaging , Choroid/pathology , Choroid/diagnostic imaging , Tomography, Optical Coherence/methods , Fundus Oculi , Lens, Crystalline/pathology , Lens, Crystalline/diagnostic imaging
6.
Korean Journal of Ophthalmology ; : 206-213, 2016.
Article in English | WPRIM | ID: wpr-50641

ABSTRACT

PURPOSE: To compare the clinical and anterior segment anatomical features in primary angle closure sub-groups based on configurations of iris root insertion. METHODS: Primary angle closure patients were imaged using anterior segment optical coherence tomography. Anterior chamber depth, iris curvature, iris thickness (IT) at the scleral spur and 500, 750, and 1,500 µm from the scleral spur (IT(0), IT(500), IT(750), and IT(1500)), lens vault, iris area, angle opening distance (AOD(500)), angle recess area (ARA(750)), and trabecular iris space area (TISA(750)) were measured. Iris root insertion was categorized into a non-basal insertion group (NBG) and basal insertion group (BG). RESULTS: In total, 43 eyes of 39 participants belonged to the NBG and 89 eyes of 53 participants to the BG. The mean age of participants was greater in the NBG than the BG (62.7 ± 5.7 vs. 59.8 ± 7.3 years, p = 0.043), and the baseline intraocular pressure was higher in the BG than the NBG (16.4 ± 4.4 vs. 14.9 ± 3.3 mmHg, p = 0.037). The BG showed a greater IT(0) (0.265 ± 0.04 vs. 0.214 ± 0.03 mm, p < 0.001) and iris area (1.59 ± 0.24 vs. 1.52 ± 0.27 mm2, p = 0.045), lower ARA(750) (0.112 ± 0.08 vs. 0.154 ± 0.08 mm2, p = 0.017) and AOD(500) (0.165 ± 0.07 vs. 0.202 ± 0.08 mm, p = 0.014) compared to the NBG. CONCLUSIONS: The BG had a narrower anterior chamber angle, thicker peripheral iris, and higher pretreatment intraocular pressure.


Subject(s)
Female , Humans , Male , Middle Aged , Anterior Eye Segment/diagnostic imaging , Glaucoma, Angle-Closure/diagnosis , Gonioscopy , Intraocular Pressure , Iridectomy/methods , Iris/diagnostic imaging , Lens, Crystalline/diagnostic imaging , Prospective Studies , Tomography, Optical Coherence/methods
7.
Indian J Ophthalmol ; 2012 Jan; 60(1): 63-65
Article in English | IMSEAR | ID: sea-138794

ABSTRACT

Blunt ocular trauma in the elderly can result in anterior dislocation of the crystalline lens into the subconjunctival space (phacocele). Although rare, this presentation can be missed, especially if the patient presents several days after the injury and if the lid is not everted on examination. While a careful clinical examination is adequate in the diagnosis, imaging techniques can be put to use for the accurate location of the associated sclera rupture. We report three cases of post-traumatic phacocele wherein ultrasound biomicroscopy (UBM) was compared to the anterior segment optical coherence tomography (AS-OCT) and B-scan ultrasonography (B-scan), in order to establish the best imaging tool for this condition. We concluded, based on image quality, that UBM could be the imaging modality of choice to aid in the diagnosis of phacocele.


Subject(s)
Aged , Anterior Eye Segment/injuries , Anterior Eye Segment/diagnostic imaging , Child , Diagnosis, Differential , Eye Injuries/complications , Eye Injuries/diagnosis , Female , Follow-Up Studies , Humans , Lens Subluxation/diagnosis , Lens Subluxation/etiology , Lens, Crystalline/injuries , Lens, Crystalline/pathology , Lens, Crystalline/diagnostic imaging , Microscopy, Acoustic/methods , Tomography, Optical Coherence/methods
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