ABSTRACT
Para evaluar la madurez del sistema inmune en recién nacidos que por motivos diagnósticos lo requieren, es imprescindibles conocer los parámetros normales. Conociendo la importancia del factor inhibidor de de la migración de los leucocitos(FIML) y al no tener referencias en cuanto a la producción de esta linfoquina en la etapa neonatal, se decidió comparar la capacidad que tienen los linfocitos T de producir FIML, cuando han sido estimulados con antígenos y con el mitógeno fitohemaglutinina en individuos adultos sanos y niños recién nacidos por parto normal, y en estos últimos se explora la inhibión de la migración de los leucocitos de sangre de cordón umbilical incubados con FIMI, específico para un antígeno. Se concluyó que los recién nacidos no responden a los antígenos de memoria y sí lo hacen a la fitohemaglutinina y al FIML, específico para un antígeno
Subject(s)
Infant, Newborn , Adult , Humans , Leukocyte Migration-Inhibitory Factors/biosynthesis , Lymphocytes/metabolism , PhytohemagglutininsABSTRACT
Nonspecific immune parameters such as natural killer(NK) activity, antibody-dependent cellular cytotoxicity(ADCC), production of leukocyte migration inhibitory factor(LlF) and levels of immune complex(IC) were assessed in 47 patients with rheumatoid arthritis (RA) 20 with degenerative arthritis (DA) and 40 healthy controls. Peripheral blood (PB) as well as synovial fluid (SF) were collected from both RA and DA patients before treatment. Mononuclear cell suspensions and sera were prepared and submitted for the in vitro tests; 4-hr chromium-release assays using human K562 and mouse L1210 cells as targets for NK and ADCC assays respectively, 2-step agarose assay for LIF and platelet aggregation test for IC. Results revealed that 1) LIF activity of PB lymphocytes (PBL) from both RA and DA patients showed a significant (P < 0.05) decrease as compared with that from healthy controls. 2) PB-NK activity from RA patients showed an insignificant decrease as compared with that from DA or healthy controls. However, mononuclear cells isolated from SF (SFL) of RA patients exhibited significantly(P < 0.02) lower NK activity than PBL from the same patients. 3) In ADCC assays with PBL no significant differencies were observed among the 3 groups. 4) Higher titers of IC were detected in both PB and SF from RA patients than DA, and a negative correlation was found between serum IC levels and PB-NK activity. These data are discussed in light of previous reports, and a hypothesis regarding a decreased nonspecific cell-mediated immunity in conjunction with an increased humoral immune response, particularly in local sites, is proposed as one of the mechanisms underlying the pathogenesis of RA.
Subject(s)
Adult , Female , Humans , Male , Antibody-Dependent Cell Cytotoxicity , Antigen-Antibody Complex/immunology , Arthritis, Rheumatoid/immunology , Killer Cells, Natural/immunology , Leukocyte Migration-Inhibitory Factors/biosynthesis , Middle Aged , Synovial Fluid/immunologyABSTRACT
The supernatants of peripheral blood lymphocytes of tuberculin-sensitive guinea pigs incubated with PPD were investigated using macrophage and leukocyte migration inhibition tests. Inhibition as well as stimulation of cell migration were observed. The effect upon migration cultures seemed to be dependent upon the immunological state of the host; animals of the V-group (vaccinated once without challenge) showed inhibitory activity to both macrophages and leukocytes, while those in the VC-group (vaccinated and challenged) had stimulatory activity only to leukocytes. The addition of antithymocyte serum stopped all activity (macrophage and leukocyte inhibition and leukocyte stimulation), suggesting that thymus-dependent lymphocytes are necessary for such activity.