ABSTRACT
In light of related methods in Chinese Pharmacopoeia(2020 edition), this study established the quality standard for Lobeliae Chinensis Herba. The TLC identification method was established with silica gel GF_(254) thin layer plate, diosmin standard, linarin standard, and the reference material of Lobeliae Chinensis Herba. The loss on drying, total ash, acid-insoluble ash, and ethanol-soluble extracts of 18 batches of Lobeliae Chinensis Herba samples were determined according to the general principles in Chinese Pharmacopoeia. Then, HPLC was adopted in the establishment of characteristic chromatogram and content determination. The results showed that the established method can achieve good separation for diosmin, linarin, and lobetyolin. Based on the results of detection for 18 batches of Lobeliae Chinensis Herba samples, the draft quality standard was established, which was expected to provide reference for the revision of this medicinal herb in Chinese Pharmacopoeia.
Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/standards , Lobelia/chemistry , Plants, Medicinal/chemistryABSTRACT
Lobelia inflata es una planta que se ha usado en medicina desde el siglo XVIII; tiene una larga e interesante historia porque posee diversos efectos medicinales y su alcaloide principal, la lobelina, se ha usado como medicina para el asma, como analéptico respiratorio y también para dejar de fumar. En la actualidad, aún se está investigando como terapia para el abuso de narcóticos y alcohol. Se introdujo como medicamento homeopático desde el siglo XIX. Sin embargo, en la actualidad es muy poco usada, a pesar de tener grandes posibilidades curativas. No se encuentra disponible una materia medica1 completa sobre ella que contenga no solo los conocimientos homeopáticos, sino aquellos que la medicina alopática ha acumulado sobre esta planta en más de doscientos años. Se realizó un trabajo de tipo cualitativo documental donde se hizo una revisión no sistemática de la información. Se revisó la literatura alopática más importante sobre la planta Lobelia inflata, la lobelina y las principales materias medicas homeopáticas y el repertorio homeopático. Se realizó una materia medica homeopática del medicamento Lobelia inflata, a partir de la revisión de varias materias medicas de diferentes autores y también de los aportes de la investigación científica alopática. Se encontró una riqueza muy grande de síntomas característicos mentales, generales y locales que pueden ser aplicados en una gran diversidad de cuadros clínicos.
Subject(s)
Humans , Homeopathic Remedy , Lobelia/therapeutic use , Materia Medica , Allopathic PracticesABSTRACT
The piperidine alkaloid composition from young stems of Lobelia polyphylla Hook & Arn. was determined by gas chromatography mass spectrometry (GC-MS). The tentative structures, without the stereochemistry, were obtained by the analysis of the fragmentation patterns of the mass spectra of each compound. The stems contained a mixture of lobeline (1), norlobelanidine (2), 1-(1-(2-hydroxy-2-phenylethyl)-1-methylpiperidin) butane-2-ol (3), 8-propyl-10-phenyl lobelionol (4), 1-(6-(2-hydroxy-2-phenylethyl)-1-methylpiperidin) butane-2-one (5), 1-(6-(2-hidroxypentyl)-1-ethylpiperidin) butane-2-one (6) and 1-methyl-2-piperidinemethanol (7). The role of these alkaloids in the toxic, narcotic and hallucinogenic effects, produced after smoking the aerial parts of this species is discussed.
La composición de alcaloides piperidínicos de tallos jóvenes de Lobelia polyphylla Hook & Arn. se determinó por cromatografía de gases acoplada a espectrometría de masas (CG-EM). Las estructuras tentativas sin incluir la estereoquímica, se obtuvieron mediante el análisis de los patrones de fragmentación de los espectros de masas de cada compuesto. Los tallos contienen una mezcla de lobelina (1), norlobelanidina (2), 1-(1-(2-hidroxi-2-feniletil)-1-metilpiperidin) butano-2-ol (3), 8-propil-10-fenil lobelionol (4), 1-(6-(2-hidroxi-2-feniletil)-1-metilpiperidin) butano-2-ona (5), 1-(6-(2-hidroxypentyl)-1-etilpiperidin) butano-2-ona (6) y 1-metil-2-piperidinmetanol (7). Se discute el posible papel de estos alcaloides en los efectos tóxicos, estupefacientes y alucinógenos, producidos después de haber fumado la parte aérea de esta especie.
Subject(s)
Alkaloids/analysis , Lobelia/chemistry , Piperidines/analysis , Plant Stems/chemistry , Gas Chromatography-Mass SpectrometryABSTRACT
Research has demonstrated that many chemical constituents dominated by piperidine alkaloids and flavonoids, such as lobelanidine, lobeline, and lobelanine, have been obtained from Lobelia chinensis Lour. Experimental studies and clinical applications have also indicated that L. chinensis possesses a number of pharmacological activities (e.g., diuretic, choleretic, breathing excitement, anti-venom, anti-bacterial, and anticancer). This paper focuses on the properties, chemical constituents, and anticancer activity of L. chinensis to clarify the connection among them, and identify the active anticancer compounds. This work serves as the foundation for further research and development of L. chinensis.
Subject(s)
Animals , Humans , Alkaloids , Pharmacology , Therapeutic Uses , Antineoplastic Agents, Phytogenic , Pharmacology , Therapeutic Uses , Flavonoids , Pharmacology , Therapeutic Uses , Lobelia , Chemistry , Neoplasms , Drug Therapy , Phytotherapy , Plant Extracts , Pharmacology , Therapeutic UsesABSTRACT
<p><b>OBJECTIVE</b>To study on the chemical constituents of Lobelia chinensis.</p><p><b>METHOD</b>The coloumn chromatographic techniques were applied to isolate constituents, and their structures were elucidated by means of spectral data analysis.</p><p><b>RESULT</b>Sixteen compounds were isolated and identified as daucosterol (1), diosmetin (2), apigenin (3), chrysoeriol (4), loteolin (5), hesperidin (6), loteolin-7-O-beta-D-glucoside (7), apigenin-7-O-beta-D-glucoside (8), linarin (9), diosmin(10), 5,7-dimethoxy-8- hydroxycoumarin (11), palmitinic acid (12), lacceroic acid (13), stearic acid (14), beta-sitosterol (15), daucosterol (16).</p><p><b>CONCLUSION</b>All of these compouds were obtained from L. chinensis for the first time.</p>
Subject(s)
Apigenin , Cholestenones , Flavones , Flavonoids , Glycosides , Hesperidin , Lobelia , Chemistry , Plant Extracts , Pharmacology , SitosterolsABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from Lobelia chinensis.</p><p><b>METHOD</b>The constituents were extracted with 95% EtOH, partitioned with different solvents, and isolated and purified by silica gel column chromatography and crystallization, their structures were elucidated by physico-chemical properties and spectroscopic data.</p><p><b>RESULT</b>Seven compounds were isolated from the titled plant. They were identified as 5-hydroxy-4'-methoxyflavone-7-O-rutinoside (1), n-butyl-O-beta-D-fructopyranoside (2), 5,7-dimethoxycoumarin (3), cirsiumaldehyde (4), diosmetin (5), 5-hydroxymethyl-2-furancarboxaldehyde (6), and 6-hydroxy-7-methoxycoumarin (7).</p><p><b>CONCLUSION</b>Compounds 2-4, 6, and 7 were obtained from the titled plant for the first time.</p>
Subject(s)
Coumarins , Furans , Lobelia , Chemistry , Plant Extracts , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To make qualitative analysis on alkaliod in Lobelia sessilifolia.</p><p><b>METHOD</b>The ESI-MS(n) method was used for qualitative analysis.</p><p><b>RESULT</b>The structures of two alkaliods in plant extract of L. sessilifolia were successfully analyzed and identified as norlobelanine and 8, 10-diethyllobelidione by electrospray ionization multistage mass spectrometry in positive mode. Then the fragmentation mechanisms were explored.</p><p><b>CONCLUSION</b>SPE and ESI-MS(n) could simply and rapidly provide qualitative examination of alkaliods in L. sessilifolia.</p>
Subject(s)
Alkaloids , Drugs, Chinese Herbal , Lobelia , Chemistry , Solid Phase Extraction , Spectrometry, Mass, Electrospray IonizationABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.</p><p><b>METHOD</b>C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.</p><p><b>RESULT</b>Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.</p><p><b>CONCLUSION</b>A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.</p>