ABSTRACT
En esta investigación se relacionaron los niveles de zinc sérico y las subpoblaciones linfocitarias T CD4(+)y T CD8(+) de un grupo de adultos mayores residenciados en un hogar geriátrico, a fin de evaluar la interacción entre este nutriente y la respuesta inmune. Se evaluaron 65 adultos mayores de 60 años, 33 del sexo femenino y 32 del sexo masculino. Se estimó consumo dietario según pesada directa individual, se determinó zinc sérico por espectrofotometría de absorción atómica y subpoblaciones T CD4 y T CD8 por citometría de flujo. El consumo calórico fue adecuado y el dezinc deficiente. El valor promedio de zinc sérico fue de 73,4 ± 11,6 μg/dl, el porcentaje de la subpoblación CD4 fue de 35,7± 10,4 y el de CD8 35,2 ± 11,0. El 55 por ciento de los ancianos evaluados estaban hipozincémicos mientras el porcentaje de valores subnormales de CD4 y CD8 se observo en el 64 por ciento y 41 por ciento respectivamente. No se observó asociación entre déficit de zinc y subpoblaciones linfocitarias. Los ancianos evaluados presentan alteraciones en su inmunocompetencia, sin asociación entre los niveles de zinc y las subpoblaciones linfocitarias.
To establish relationship between serum zinc levels and CD4 and CD8 lymphocyte subpopulations in a group of elderly living in a geriatric home. Material and Methods: 65 elderly (33 female and 32 male) older than 60 years were evaluated. Dietary intake by weighed method, serum zinc by atomic absorption spectrophotometry and CD4 and CD8 lymphocyte subpopulations by flow cytometry. Results: Caloric intake was adequate while zinc intake was deficient. Mean serum zinc value was 73,4±11,6 μg/dl. CD4 T lymphocyte value was 35,7±10,4 percent and CD8 was 35,2 ±11,0 percent. It was found that 55 percent of the elderly were hypozincemic, while 64 percent and 41percent had low percentages of CD4 and CD8 lymphocytes, respectively. No association between zinc levels and subpopulations was observed. Elderly showed diminished lymphocytes which could cause compromise of immunecompetence but no association with zinc levels was found.
Subject(s)
Humans , Male , Adult , Female , Lymphocyte Subsets/chemistry , Zinc/analysis , Nutritional SciencesABSTRACT
The aim of this project was to compare dual and tri-colour reagents for lymphocyte immunophenotyping. A total of 37 patient and normal specimens were immunophenotyped concurrently with the following mean values (% dual vs tri-colour): CD3 (69.4 vs 68.3) CD4 (24.0 vs 24.2) and CD19 (13.9 vs 12.6). A comparison of the results obtained using the paired t test showed that there were no significant differences for cells expressing CD3, CD4 and CD19. However, there was a significant difference in the NK (18.3 vs 16.3) cell component. A major advantage in using 3 colour immunophenotyping is the ability to analyse specimens that cannot be analysed using dual colour reagents due to debris or contamination of the gate with non-lymphocytic cells.
Subject(s)
Antigens, CD19/analysis , CD3 Complex/analysis , CD4 Antigens/analysis , Coloring Agents , Flow Cytometry/methods , HLA-DR Antigens/analysis , Humans , Indicators and Reagents , Killer Cells, Natural/chemistry , Lymphocyte Subsets/chemistryABSTRACT
The present study was conducted in order to investigate the immunologic alterations alongside the numerical changes in peripheral blood lymphocytes(PBL) and their subsets in stomach cancer patients. Lymphocyte surface markers were determined in 85 stomach cancer patients and 49 controls by indirect immunofluorescence technique using monoclonal antibodies. Monoclonal antibodies used were Leu 2a(CD8, suppressor/cytotoxic T cells), Leu 3a(CD4, inducer/helper T cells), Leu 4(CD3, pan T reagent), Leu 11(CD16, natural killer cells) and Leu 12(CD19, B cells). The numbers of PBL, CD3+, CD4+, CD8+, CD16+ and CD19+ cells significantly decreased and the CD4: CD8 value increased in 85 patients with stomach cancer compared to those in controls(p < 0.01). In stage I(n = 17), neither PBL, their subsets nor the CD4: CD8 value were significantly different from those of the controls. In stage II(n = 17), the numbers of PBL, CD3+, CD4+ and CD8+ cells decreased(p < 0.01). In stage III(n = 24) and IV(n = 27), PBL and all subsets measured decreased(p < 0.01). The CD4: CD8 value showed significant increases in stages III and IV(p < 0.01), because the CD8+ cells decreased to a greater extent than did the CD4+ cells. The results demonstrating that the lymphocyte subsets are depressed differentially with the stage suggest that host immunity is impaired with the progression of stomach cancer.