Subject(s)
Humans , Immunotherapy, Adoptive/methods , Lymphoma, Large B-Cell, Diffuse/therapy , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/therapy , Receptors, Chimeric Antigen/therapeutic use , Immunotherapy, Adoptive/instrumentation , Immunotherapy, Adoptive/trends , Lymphoma, Large B-Cell, Diffuse/immunology , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/immunology , Receptors, Chimeric Antigen/chemical synthesisABSTRACT
Human immunodeficiency virus (HIV) infection is a risk factor for developing non-Hodgkin's lymphoma. Most acquired immune deficiency syndrome (AIDS)-related lymphomas are high-grade B cell non-Hodgkin's lymphomas. The use of highly active antiretroviral therapy has reduced the incidence of AIDS-related lymphoma. There have been 7 reports of AIDS-related extra-nodal lymphoma in Korea. We report a case of AIDS-related lymphoma detected by MiroCam(R) capsule endoscopy.
Subject(s)
Humans , Male , Middle Aged , Acquired Immunodeficiency Syndrome/complications , Anti-HIV Agents/therapeutic use , Antiretroviral Therapy, Highly Active , Capsule Endoscopes , HIV Infections/virology , Jejunal Neoplasms/diagnosis , Lymphoma, AIDS-Related/diagnosis , Lymphoma, Large B-Cell, Diffuse/immunology , Tomography, X-Ray ComputedABSTRACT
AIM: To determine EBNA-1 expression in the tissue of patients with diffuse large B-cell lymphoma and its relationship to bcl-2 expression. METHODS: Paraffin-embedded tissue from 24 cases of diffuse large B-cell lymphoma were stained immunohisto chemically with monoclonal antibody anti-EBNA-1 and anti-bcl-2 using Streptavidin Biotin Complex method. The bcl-2 expression was measured as negative (no staining), positive 1 (weak staining), and positive 2 (moderate to strong staining). The relationship between the immunohisto chemistry results was examined. RESULTS: From the 24 samples, 12 (50%) were EBNA-1 positive and 14 (58.3%) showed expression of bcl-2. Eleven of the 14 samples (90%) showed expression of bcl-2 were EBNA-1 positive, while only 3 of these samples (10%) were EBNA-1 negative. The relationship between bcl-2 expression and EBNA-1 was statistically significant (Chi square-uncorrected 11.2571, p= 0.0036). CONCLUSION: Our results showed that 50% of diffuse large B-cell lymphoma were EBNA-1 positive. Furthermore, EBNA-1 seemed to be correlated with bcl-2 expression.
Subject(s)
Adult , Aged , Cross-Sectional Studies , Epstein-Barr Virus Nuclear Antigens/metabolism , Female , Humans , Immunohistochemistry , Lymphoma, B-Cell/immunology , Lymphoma, Large B-Cell, Diffuse/immunology , Male , Middle Aged , Proto-Oncogene Proteins c-bcl-2/metabolismABSTRACT
E método de inmunofijación simplificado ha resultado útil en la identificación de anormalidades monoclonales no caracterizadas por inmunoelectroforesis. Este métodos permitió resolver 3 de 19 muestras de suero procesados, con una alteración monoclonal no caracterizada completamente por inmunoelectroforesis. Basados en estos resultados y en la revisión de la literatura, se propone el método de inmunofijación simplificado para la identificación y caracterización de anormalidades monoclonales, cuando éstas no son satisfactoriamente clasificadas por inmunoelectroforesis