ABSTRACT
OBJETIVO: Desenvolver um método de diagnóstico diferencial de afogamento, pela análise quantitativa dos macrófagos alveolares, em ratos submetidos à afogamento induzido em água doce e água salgada. MÉTODOS: Foram utilizados 15 ratos adultos, machos Wistar EPM-1, pesando 360g (DP=21,3), randomizados em três grupos: G1 controle; G2 Água doce; G3 Água salgada, cada um com n=5. Os animais foram anestesiados e traqueostomizados para inserção de uma cânula endotraqueal, para indução do afogamento. Os pulmões foram removidos, pesados, preparados para histologia e corados por imunohistoquímica. Os macrófagos foram contados em ambos os pulmões (direito e esquerdo) de cada animal. O teste estatístico utilizado foi o ANOVA (SPSS.10) com p<0,05.
Subject(s)
Animals , Male , Rats , Drowning/diagnosis , Fresh Water/analysis , Immunohistochemistry/methods , Lung/pathology , Macrophages, Alveolar/pathology , Seawater/analysis , Analysis of Variance , Case-Control Studies , Diagnosis, Differential , Disease Models, Animal , Forensic Pathology/methods , Lung/chemistry , Macrophages, Alveolar/chemistry , Organ Size , Random Allocation , TracheostomyABSTRACT
The search for lipid laden macrophages in sputum could be a simple diagnosis method for pulmonary aspiration. To assess the usefulness of sputum lipid laden macrophages in the diagnosis of pulmonary aspiration, 16 children aged 0 to 4 years old, admitted with aspiration pneumonia were studied. The diagnosis was based on clinical grounds (vomiting or radiologically diagnosed swallowing abnormalities plus persistent pulmonary condensations) or radionucleide milk scanning. As controls, 6 children of 1 to 5 years old, admitted for elective tonsillectomy, were studied. Sputum was stained with Sudan III-Hematoxylin, 100 macrophages were read and each cell received a score from 0 to 4 according to the degree of ataining. Lipid laden macrophages of children with aspiration pneumonia had a mean score of 150ñ107 (8-323) compared with those of controls that had a score of 1.3ñ1.5 (0-4). The study of sputum lipid laden macrophages may be a useful diagnostic method for aspiration pneumonia