ABSTRACT
In these work the in vitro antioxidant activity and the in vivo genotoxicity of M. dasyclada was compared to the reference species M. aquifolium and M. ilicifolia. M. dasyclada showed in vitro antioxidant activity comparable to M. aquifolium but lower than M. ilicifolia, being that a inverse Pearson correlation between DPPH IC50 values and total phenolic content was detected (–0.932). The carbonyl content of M. dasyclada and M. aquifolium extracts promoted a similar increase in protein oxidation in vivo, while M. ilicifolia no altered the carbonyl levels. The comet assay demonstrated that the three analyzed species promoted a low and similar level of genotoxicity; which is compatible with DNA damage induced by other medicinal plants and is partially recovered by a co-treatment with vitamin C. The data showed M. dasyclada as antioxidant activity in vitro, and that its genotoxic and pro-oxidant effects in vivo are comparable to the Maytenus reference species.
No presente trabalho a atividade antioxidante in vitro e a genotoxicidade in vivo de M. dasyclada foi comparada com as espécies de referência M.aquifolium e M. ilicifolia. M. dasyclada mostrou atividade antioxidante in vitro comparável a de M. aquifolium mas inferior a M. ilicifolia, sendo que foi detectada uma correlação de Pearson inversa entre os valores de IC50 por DPPH e o conteúdo fenólico total (–0,932). Em relação ao teor de carbonila, os extratos de M. dasyclada e M. aquifolium promoveram um aumento semelhante na oxidação das proteínas in vivo, ao passo que Maytenus ilicifolia não alterou os níveis de carbonila. O ensaio do cometa demonstrou que as três espécies analisadas promoveram um nível baixo e semelhante de genotoxicidade, o que é compatível com os danos no DNA induzidos por outras plantas medicinais e é parcialmente recuperada por um co-tratamento com a vitamina C. Os dados mostraram M. dasyclada com atividade antioxidante in vitro, e que os seus efeitos genotóxicos e pró-oxidantes in vivo são comparáveis às espécies de referência de Maytenus.
Subject(s)
Animals , Male , Antioxidants/pharmacology , Maytenus/chemistry , Plant Extracts/pharmacology , Plants, Medicinal/chemistry , Antioxidants/toxicity , Comet Assay , Maytenus/classification , Maytenus/toxicity , Plant Extracts/toxicity , Plants, Medicinal/classification , Plants, Medicinal/toxicity , Rats, WistarABSTRACT
The petroleum ether fraction of the aerial parts of Maytenus arbutifolia upon repeated chromatography afforded five compounds identifies as: beta -amyrin - 3 - tridecanoic acid ester [1], beta -amyrin [2], beta -sitosterol [3], lupeol oxide [4] and beta -sitosterol glucoside [5], while from the ethyl acetate fraction [-] epicatechin [6] was isolated. The structures of these compounds were established on bases of spectral [UV, IR, MS, [1]H and [13]C-NMR] as well as physical and chemical data. Antimicrobial activity for extracts indicated weak potency [200 micro g/ml]. The cytotoxic activity of all extracts conducted by the brine shrimp and the cell lines as well showed the same IC[50] [micro g/ml] on Hep-2 cell, whereas, the different extracts showed different activity on Hela cell [ethanol> ethyl acetate> pet.ether; IC[50] 103.5, 108.5 and 142 micro g/ml, respectively]. The Pharmacological activity of the different extracts showed sedative effect; slowing of respiration; dose-dependent decrease In arterial blood pressure by 40.1 mm Hg [at a dose 200 mg/kg]; decrease in the body temperature by 2.1[degree sign]C after 2hr [at a dose 120mg/kg]. The anti-inflammatory activity of the ethanolic extract [0.39 g/kg, i.p.] using formalin - induced oedema in rat hind paw, produced a significant dose-dependent anti-inflammatory effect: 19.6 +/- 9.1, 30.5 +/- 4.3 and 43.3 +/- 3.9% after 1, 2 and 3 hr. respectively