ABSTRACT
Our objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of nerve repair: 1) epineural neurorrhaphy using sutures alone (group S - 10 rats), 2) epineural neurorrhaphy using sutures plus fibrin tissue adhesive (FTA; group SF - 20 rats), and 3) sutures plus FTA, with MDP added to the FTA (group SFM - 20 rats). Functional assessments using the grasp test were performed weekly for 12 weeks to identify recovery of flexor muscle function in the fingers secondary to median nerve regeneration. Histological analysis was also utilized. The total number and diameter of myelinated fibers were determined in each proximal and distal nerve segment. Two indices, reported as percentage, were calculated from these parameters, namely, the regeneration index and the diameter change index. By the 8th week, superiority of group SFM over group S became apparent in the grasping test (P = 0.005). By the 12th week, rats that had received MDP were superior in the grasping test compared to both group S (P < 0.001) and group SF (P = 0.001). Moreover, group SF was better in the grasping test than group S (P = 0.014). However, no significant differences between groups were identified by histological analysis. In the present study, rats that had received MDP obtained better function, in the absence of any significant histological differences.
Subject(s)
Animals , Male , Rats , Acetylmuramyl-Alanyl-Isoglutamine/pharmacology , Adjuvants, Immunologic/pharmacology , Fibrin Tissue Adhesive/pharmacology , Nerve Regeneration/drug effects , Median Nerve/drug effects , Median Nerve/physiology , Nerve Regeneration/physiology , Rats, Wistar , Sutures , Time FactorsABSTRACT
Somatosensory evoked potentials (SEPs) on median nerve stimulation were studied in 38 rubber factory workers to detect possible subclinical impairments in the somatosensory pathway as a result of exposure to rubber factory chemicals. Results showed trend towards the prolongation of latencies of all the major peaks of SEP such as P1, N1, P2, N2, P3, N3 in exposed workers besides changes in peak to peak amplitudes (higher P1-N1 and lower N2-P3). These changes may be attributed to synergistic action of solvents and other rubber factory chemicals on the generators of SEPs located higher up at the thalamo-cortical level.