ABSTRACT
Apesar de a heparina ser amplamente utilizada como agente anticoagulante, suas funçoes biológicas permanecem incertas. Além do papel na coagulaçao, esse sacarídeo parece modular respostas imunológicas e inflamatórias. Com o objetivo de avaliar-se o efeito da heparina sobre o sistema mononuclear fagocitário, realizou-se este trabalho em nível experimental. Foram utilizadas 24 ratas divididas em dois grupos: Grupo I - administraçao de soluçao salina a O,9 por cento e Grupo II - administraçao de heparina sódica. Os animais foram acompanhados durante 26 dias. Decorrido esse prazo, administrou-se soluçâo de enxofre coloidal marcado com 99 mTc, por via venosa. Após trinta minutos, foram retirados segmentos do figado, baço e pulmao, além de um coágulo sangüíneo, que foram analisados através de cintilografia. Houve maior captaçao do radiofármaco pelos baços de ambos os grupos. Nao houve diferença entre os registros cintilográficos dos dois grupos, porém a depuraçao sangüínea do colóide foi menor em presença de heparina. Concluindo, a heparina parece interferir com a depuraçao sangüínea de colóide e tende a mudar o padrao de captaçao do sistema mononuclear fagocitário.
Subject(s)
Animals , Female , Rats , Spleen , Blood , Liver , Heparin/pharmacology , Mononuclear Phagocyte System/drug effects , Phagocytes/drug effects , Phagocytosis/drug effects , Lung , Analysis of Variance , Rats, Sprague-DawleyABSTRACT
The clinical material for our studies of serum total LDH activity and LDH isoenzymes in leprosy included 255 patients consisting of Tuberculoid (74), Lepromatous leprosy (116), and Lepromatous leprosy with lepra reaction (65). 20 patients with suspected DDS resistance and repeated attacks of lepra reactions were selected for Clofazimine studies. All leprosy patients exhibited higher total LDH activity as compared to normals. M/H ratio was significantly increased in patients of Lepromatous leprosy and correlated closely with the clinical severity and advancement of disease. Tuberculoid leprosy patients showed values close to normals. Hence M/H ratio could demarcate two polar types of leprosy i.e. Tuberculoid and Lepromatous leprosy. Clofazimine treatment over a period of one year in patients with suspected DDS resistance and repeated attacks of lepra reaction decreased total LDH activity and M/H ratio considerably. Fall in M/H ratio during Clofazimine treatment could be attributed to the clearance of 'M' subunits by the drug due to removal of blockade of R.E.S. system produced by lepra bacilli.
Subject(s)
Clofazimine/pharmacology , Humans , Isoenzymes , L-Lactate Dehydrogenase/analysis , Leprosy/enzymology , Mononuclear Phagocyte System/drug effectsABSTRACT
Calciphylactic blockade of Reticulo endothelial system (REB) was used to study the role of reticulo-endothelial system (RES) in hepatic regeneration. REB was induced by i.v. egg yolk administration in DHT sensitized animals, which were subsequently partially hepatectomized. Mitotic index and percentage regeneration were studied in these animals 48 hr after partial hepatectomy. REB produced a significant depression of the mitotic index and percentage regeneration in regenerating livers. This suggests that functional integrity of the RES in essential hepatic parenchymal proliferation after partial hepatectomy.