ABSTRACT
INTRODUCCIÓN: el género Morus constituye una fuente importante de sustancias bioactivas de diversa naturaleza química. Además, el uso de Morus alba L., planta conocida como morera, se ha diversificado gracias a que posee excelentes cualidades nutricionales. En Cuba, sobresale como fuente de forraje por su capacidad de producción de biomasa, composición química, alta degradabilidad, adaptabilidad a diversas condiciones de clima y suelo, perennidad ante el corte y disponibilidad. OBJETIVOS: realizar el tamizaje fitoquímico de 10 variedades e híbridos de M. alba y evaluar la toxicidad de los extractos acuosos en ratas de laboratorio. MÉTODOS: se recolectaron las hojas frescas. Se lavaron, desinfectaron, secaron, y pulverizaron y se extrajeron con n-hexano, etanol y agua. Los extractos se filtraron y se les realizaron las pruebas fitoquímicas de identificación. En ratas se realizó una evaluación de toxicidad de los extractos acuosos para la determinación preliminar de su inocuidad. RESULTADOS: el estudio fitoquímico mostró considerables cantidades de triterpenos y esteroides, así como fenoles y taninos en los extractos evaluados, mientras que no fueron detectados quinonas ni alcaloides. En el ensayo de toxicidad no se manifestaron síntomas clínicos importantes como mortalidad, convulsiones, alteraciones en el ritmo cardiaco o respiratorio. CONCLUSIONES: el análisis fitoquímico de las variedades e híbridos de M. alba, mostraron abundantes cantidades de metabolitos secundarios descritos como agentes antioxidantes. El estudio de toxicidad realizado mostró una inocuidad del extracto acuoso en ratas, por lo que estos extractos sugieren la presencia de propiedades farmacológicas para el tratamiento de numerosas enfermedades asociadas al estrés oxidativo.
INTRODUCTION: the Morus genus constitutes an important source of bioactive substances of diverse chemical nature. In addition, the use of Morus alba L., plant known as mulberry, has been rapidly diversified because it has excellent nutritional qualities. In Cuba, it stands out as forage source due to its biomass production capacity, chemical composition, high degradability, adaptability to different climate and soil conditions, perennial character when pruned and availability. OBJECTIVES: to perform the phytochemical sieving of 10 M. alba varieties and hybrids and evaluate the toxicity of the aqueous extracts in laboratory rats. METHODS: the fresh leaves were collected. They were washed, disinfected, dried, and pulverized and extracted with n-hexane, ethanol and water. The extracts were filtered and the phytochemical identification tests were ran on them. A toxicity evaluation of the aqueous extracts was conducted in rats for the preliminary determination of their innocuousness. RESULTS: the phytochemical study showed considerable quantities of triterpenes and steroids, as well as phenols and tannins in the evaluated extracts, while neither quinones nor alkaloids were detected. In the toxicity trial no important clinical symptoms, such as mortality, convulsions, or alterations of the heart or respiratory rate, appeared. CONCLUSIONS: the phytochemical analysis of the M. alba varieties and hybrids showed abundant quantities of secondary metabolites described as antioxidant agents. The toxicity study conducted showed innocuousness of the aqueous extract in rats, for which these extracts suggest the presence of pharmacological properties for the treatment of many diseases associated to the oxidative stress.
Subject(s)
Rats , Plant Preparations/therapeutic use , Morus , Morus/drug effectsABSTRACT
The aim of the present study was designed to study the effects of black and white mulberry juice on blood glucose, lipid profile and oxidative stress in normal and diabetic rats. Sprague Dawley male rats weighing [125 +/- 12.2 g] were divided into eight groups [each of 12 rats]. Four healthy control group include normal control, normal black mulberry, normal white mulberry and normal mixture of black and white mulberry, while the remaining four groups were induced diabetic rats by injected intraperitonelly with 100 mg/kg body weight of alloxan [diabetic control, diabetic received black mulberry, diabetic received white mulberry and diabetic received mixture of black and white mulberry]. Results show significant elevation in serum glucose [280%] in diabetic control group compared to normal control group. while administration of mulberry juice decreased serum glucose concentration by 45%, 20% and 37% in diabetic black mulberry, diabetic white mulberry and diabetic mixture mulberry, respectively as compared to diabetic control group compared to diabetic control group. However, the mulberry juice significantly increase the concentration of reduced glutathione [GSH] and erythrocyte SOD activity. Also mulberry juice significantly decrease oxidative stress which manifested by decrease the level of MDA and NO. The alloxan produced significant increase in ALT and AST, urea and creatinine in diabetic control group as compared to normal control group. While, the administration of mulberry juice decreased the levels of ALT, AST, urea and creatinine in diabetic group. However mulberry juice is the highly effective on lipid profile and atherogenic index which were manifested by decrease [TAG], TC, LDL, and atherogenic index and increase [HDL-C] as compared to diabetic control group [P<0.05]. It was concluded that the mulberry juice has considerable hypoglycemic and hypocholestronic effect and strengthening antioxidant, it able to reduce diabetic complications especially black mulberry, to rich in anthocyanin