ABSTRACT
Background: Silent presence of non-tuberculous mycobacterium (NTM) has been observed since the last 100 years, but now the increasing incidence of NTM is of great concern for clinical microbiologists as well as clinicians. Although many advanced efforts are being made for identification and control of Mycobacterium tuberculosis, still the silently growing menace of non-tuberculous mycobacteria is receiving negligible attention. Objectives: This study was aimed to find NTMs in positive cultures and identify them up to species level. Material & Methods: During the study period, i.e. from January 2009 to June 2011, a total of 4104 positive cultures were subjected to species identification by different morphological and biochemical tests. All the tests for identification were performed as per standard procedure along with the standard strains of NTM provided by JALMA, Agra. Results: The identification of positive cultures showed 4044/15581 (25.95%) Mycobacterium tuberculosis complex and 60/15581(0.38%) NTM. The mycobacterium species identification results showed that out of total 60 NTM, 21 different species of NTM were found and they belonged to all the four groups of runyon. The most common species identified in this study was M.simiae (07) followed by M.avium(06), M.gordonae(05), M.kansasii(05), M.fortuitum(05), M.chelonae(05), M.pheli(05), M.terrae(04), M.szulgai(02), M.vaccae(02), M.flavescens(02), M. trivale(02), M.malmoense(01), M.scrofulaceum(01), M.intracellulare(01), M.xenopi(01), M.ulcerans(01), M.tusciae(01), M.triplex(01), M.septicum(01), M.mucogenicum(01). Conclusion: The isolation of NTMs from different clinical samples indicated that they may be the causative agents for pulmonary and extra-pulmonary non-tuberculous diseases. Elaborate and focused studies are needed to differentiate NTMs amongst commensal/colonizer, pathogen and laboratory contaminants.
Subject(s)
Culture Media/diagnosis , Humans , India/epidemiology , Mycobacterium/analysis , Mycobacterium/classification , Mycobacterium/epidemiology , Mycobacterium/isolation & purification , Mycobacterium avium/analysis , Mycobacterium avium/isolation & purificationABSTRACT
Estudou-se a utilizaçäo da técnica de eletroforese em gel de poliacrilamida-dodecil sulfato de sódio (SDS-PAGE), na identificaçäo do gênero Mycobacterium. Treze espécies diferentes, foram utilizadas para preparar extratos de células íntegras, rompidas por ultra-som, pérolas de vidro e solubilizadas em fenol-ácido acético-água. O método do ultrassom forneceui as melhores separaçöes eletroforéticas. A extraçäo fenol-acéticafalhou em fornecer perfis adequados. A desintegraçäo mecânica por pérolas de vidro, mostrou-se exeqüível na produçäo de extratos e os perfis obtidos foram bastante distintos. As bandas de polipeptideos obtidas por SDS-PAGE foram discriminatórias e possibilitaram a identificaçäo das espécies estudadas. O M. tuberculosis e o M. bovis exibiram perfis bastante semelhantes, ao contrário das espécies M. avium, M. intracellulare e M. scrofulaceum cujos perfis foram diferentes, principalmente, desta última espécie. O M. fortuitum, M. smegmatis e M. phlei também mostraram perfis distintos. O número de polipeptídeos das espécies estudadas variou entre 20 e 46. A SDS-PAGE é um procedimento alternativo que pode ser utilizado na identificaçäo de espécies do gênero Mycobacterium.
Subject(s)
Animals , Electrophoresis, Polyacrylamide Gel , Mycobacterium , Brazil , Mycobacterium/analysisABSTRACT
Se estudian 3 cepas de micobacterias no tuberculosas aisladas de un paciente con neumopatía crónica remitidas por el laboratorio Provincial de Matanzas al Laboratorio Nacional de Referencia e Investigación en Micobacterias y Tuberculosis del IPK. Se informa la identificación por cromatografía en capa delgada del M. szulgai, especie recientemente conocida como patógena, algo semejante al M. gordonas, pero diferente en su estructura lipídica, así como por determinadas pruebas bioquímicas