ABSTRACT
In order to investigate the effect of vitamin A (VA) on the secretion of IFN-gamma and IL-4 in Mycoplasma Pneumoniae (MP)-induced A549 cells, A549 cells were co-cultured with MP for different time lengths and then the levels of IFN-gamma and IL-4 in the cell culture supernatants were detected before and after treatment with different concentrations of VA by using the enzyme-linked immunosorbent assay (ELISA). The results showed that the level of IFN-gamma and IL-4 in the supernatants of MP-induced A549 cells was much higher than that in non-induced cells (P<0.01). After application of VA, IL-4 level was not increased until the concentration of VA was up to 0.5x10(-5) mol/L (P<0.01). However, with concentration of VA increased up to 1x10(-4) mol/L, IL-4 was significantly suppressed (P<0.01). It was concluded that MP could induce the secretion of IFN-gamma and IL-4 in A549 cells. VA could inhibit the secretion of IFN-gamma and increase the IL-4 level in MP-induced A549 cells. However, high concentration of VA had an inhibitory effect on the secretion of IL-4 as well as on the IFN-gamma. These data provided a theoretical basis for the application of VA in MP pneumonia in the clinical practice.
Subject(s)
Cell Line, Tumor , Coculture Techniques , Culture Techniques , Interferon-gamma/metabolism , Interleukin-4/metabolism , Lung Neoplasms/pathology , Mycoplasma pneumoniae/growth & development , Mycoplasma pneumoniae/physiology , Vitamin A/pharmacologyABSTRACT
Mycoplasma pneumoniae es un germen patógeno frecuente del tracto respiratorio humano, especialmente en niños y adultos jóvenes. El desarrollo en los últimos años de nuevos métodos diagnósticos como el de reacción en cadena de la polimerasa (PCR), unido a métodos diagnósticos tradicionales, ha permitido ahondar en las características de la enfermedad por M. pneumoniae en pediatría.