ABSTRACT
The thalamus is an important modulator of seizures and is severely affected in cholinergic models of epilepsy. In the present study, chronically epileptic rats had their brains processed for neo-Timm and acetylcholinesterase two months after the induction of status epilepticus with pilocarpine. Both controls and pilocarpine-treated animals presented neo-Timm staining in the anterodorsal nucleus, laterodorsal nucleus, reticular nucleus, most intralaminar nuclei, nucleus reuniens, and rhomboid nucleus of the thalamus, as well as in the zona incerta. The intensity of neo-Timm staining was similar in control and pilocarpine-treated rats, except for the nucleus reuniens and the rhomboid nucleus, which had a lower intensity of staining in the epileptic group. In animal models of temporal lobe epilepsy, zinc seems to modulate glutamate release and to decrease seizure activity. In this context, a reduction of neo-Timm-stained terminals in the midline thalamus could ultimately result in an increased excitatory activity, not only within its related nuclei, but also in anatomical structures that receive their efferent connections. This might contribute to the pathological substrate observed in chronic pilocarpine-treated epileptic animals.
Subject(s)
Animals , Male , Rats , Brain Mapping , Staining and Labeling/methods , Status Epilepticus/metabolism , Thalamic Nuclei/metabolism , Nerve Endings/metabolism , Zinc/metabolism , Acetylcholinesterase , Chronic Disease , Status Epilepticus/chemically induced , Status Epilepticus/pathology , Pilocarpine , Rats, Wistar , Nerve Endings/pathologyABSTRACT
Se presenta un niño de 10 años de edad con lesiones musculosas dispuestas en arabescos y arremolinadas en tórax; lineales en miembros superiores e inferiores. Esta coexisten con afectación de SNC y compromiso óseo, dentario y ocular, constituyendo el cuadro de Hipomelanosis de Ito. Se efectúa microscopía electrónica para confirmar el diagnóstico. Se hace referencia a características clínicas y evolutivas, puntualizando las diferencias con otros síntomas neurocutáneos
Subject(s)
Humans , Child , Male , Skin Diseases, Genetic/diagnosis , Incontinentia Pigmenti/pathology , Keratinocytes/pathology , Melanocytes/ultrastructure , Nerve Endings/pathology , Pigmentation Disorders/ultrastructure , Abnormalities, Multiple , Diagnosis, Differential , Epilepsy , Incontinentia Pigmenti/diagnosis , Keratinocytes/ultrastructure , Melanocytes/pathology , Nerve Endings/ultrastructure , Neural Crest/pathology , Nevus/diagnosis , Pigmentation Disorders/diagnosis , Pigmentation Disorders/pathologyABSTRACT
Terminaçöes nervosas subepiteliais da mucosa lingual de cobaias foram estudadas pelo auxílio da técnica de impregnaçäo pela prata. Utilizou-se 15 cobaias adultas para a obtençäo das peças. Os espécimes foram fixados segundo a técnica descrita por Erhart e Rezze (1) e incluídas em parafina rotineiramente. Cortes histológicos de 8 micrometros foram montados e analisados através do microscópio Zeiss. Numerosas fibras nervosas, que apresentam trajetos diferentes, e suas terminaçöes junto à camada epitelial säo descritas