Subject(s)
Humans , Male , Infant , Orbital Neoplasms/complications , Orbital Neoplasms/diagnostic imaging , Exophthalmos/etiology , Adrenal Gland Neoplasms/pathology , Adrenal Gland Neoplasms/diagnostic imaging , Neuroblastoma/complications , Neuroblastoma/diagnostic imaging , Orbital Neoplasms/pathology , Orbital Neoplasms/secondary , Diagnosis, Differential , Neuroblastoma/pathology , Neuroblastoma/secondaryABSTRACT
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA interference on cell proliferation and invasion in vitro and in vivo. RNA interference of survivin led to a significant decrease in invasiveness and proliferation and increased apoptosis in SH-SY5Y cells in vitro. RNA interference of survivin inhibited tumor growth in vivo by 68±13% (P=0.002) and increased the number of apoptotic cells by 9.8±1.2% (P=0.001) compared with negative small interfering RNA (siRNA) treatment controls. Moreover, RNA interference of survivin inhibited the formation of lung metastases by 92% (P=0.002) and reduced microvascular density by 60% (P=0.0003). Survivin siRNA resulted in significant downregulation of survivin mRNA and protein expression both in vitro and in vivo compared with negative siRNA treatment controls. RNA interference of survivin was found to be a potent inhibitor of SH-SY5Y tumor growth and metastasis formation. These results support further clinical development of RNA interference of survivin as a treatment of neuroblastoma and other cancer types.
Subject(s)
Animals , Humans , Apoptosis/drug effects , Cysteine Proteinase Inhibitors/pharmacology , Inhibitor of Apoptosis Proteins/drug effects , Lung Neoplasms/secondary , Neuroblastoma/pathology , RNA, Small Interfering/pharmacology , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Cell Proliferation/drug effects , Down-Regulation/drug effects , Gene Expression Regulation, Neoplastic/drug effects , Immunohistochemistry , In Situ Nick-End Labeling , Inhibitor of Apoptosis Proteins/genetics , Inhibitor of Apoptosis Proteins/metabolism , Mice, Nude , Neoplasm Invasiveness , Neuroblastoma/secondary , Reverse Transcriptase Polymerase Chain Reaction , RNA, Neoplasm/drug effects , RNA, Neoplasm/metabolism , Xenograft Model Antitumor AssaysABSTRACT
Bone marrow examination has been increasingly useful in documenting metastatic involvement of tumors. A retrospective analysis of 73 cases of bone marrow metastasis of solid tumors revealed 27 cases in the pediatric age group and 46 cases in the adult age group. All 27 pediatric cases were that of neuroblastoma. In the adult bone marrow metastasis from carcinoma prostate were present in 22 cases followed by carcinoma breast in 13 cases. Rest were 5 cases of carcinoma lung, 4 cases of carcinoma colon, 1 case each of carcinoma thyroid and renal cell carcinoma. A number of associated features were observed which may help to suggest bone marrow metastasis, in the absence of tumor cells in the bone marrow.
Subject(s)
Adolescent , Adult , Aged , Bone Marrow Neoplasms/pathology , Breast Neoplasms , Child , Child, Preschool , Female , Humans , Infant , Male , Middle Aged , Neuroblastoma/secondary , Prostatic Neoplasms , Retrospective StudiesABSTRACT
Os objetivos deste estudo foram analisar uma série de neuroblastomas metastáticos na medula óssea e avaliar a sua imunorreatividade para enolase neurônio-específica (NSE) e "protein gene product 9,5" (PGP 9,5). Estudaram-se retrospectivamente 64 biópsias de medula óssea de 44 pacientes, das quais 32 apresentavam metástases de neuroblastoma. A neoplasia era constituída predominantemente por células em diferenciação em 21 casos (65,6 por cento) e por células indiferenciadas em 11 (34,4 por cento). A imuno-histoquímica foi positiva em 28 biópsias (87,5 por cento). Em neoplasias em diferenciação a maior parte da massa tumoral era imunorreativa para ambos os anti-soros, enquanto que nas neoplasias indiferenciadas não se observou imunorreatividade em até 50 por cento da massa tumoral. Concluímos que, nos casos de estadiamento da neoplasia, a análise pelos métodos rotineiros mostrou acuidade diagnóstica idêntica à da imuno-histoquímica. Este método foi útil na detecção de neoplasias residuais após o tratamento.
Subject(s)
Humans , Neuroblastoma/secondary , Bone Marrow/pathology , Bone Marrow Neoplasms/secondary , Retrospective Studies , Neuroblastoma/ultrastructure , Bone Marrow Neoplasms/ultrastructureABSTRACT
Os autores apresentam os achados radiológicos observados em um caso de neuroblastoma da glândula adrenal esquerda, com metástase osteoblásticas disseminadas. Säo discutidos os vários tipos de comprometimento ósseo pelo neuroblastoma, assim como os diagnósticos diferenciais
Subject(s)
Child, Preschool , Humans , Male , Adrenal Gland Neoplasms , Bone Neoplasms/secondary , Neuroblastoma/secondary , Diagnosis, Differential , Neoplasm Metastasis , Neuroblastoma , Prognosis , Tomography, X-Ray ComputedABSTRACT
Os autores relatam o caso de uma criança portadora de neuroblastoma metastático em boca. Comentam os seus aspectos semiológicos principais, diagnóstico diferencial, bem como os vários fatores que podem interferir diretamente no plano de tratamento e prognóstico. Além disso, chamam a atenção para o fato de que este tipo de neoplasia pode, em alguns casos, apresentar características clínicas e radiográficas semelhantes à patologias de origem odontogênica