1.
J Postgrad Med
;
1994 Jan-Mar; 40(1): 7-9
Article
in English
| IMSEAR
| ID: sea-116174
ABSTRACT
A facile and sensitive high performance liquid chromatographic (HPLC) technique has been developed for the determination pyrazinamide (PZA) in human plasma. Nicotinamide(NIA) is used as internal standard(IS). Plasma is deproteinized with 0.7 M perchloric acid; clear supernatant is neutralized with 1M NaOH and injected onto HPLC. The separation of pyrazinamide and the internal standard is carried out on a Supelco LC-18 (DB) column with a basic mobile phase. Pyrazinoic acid, the major metabolite, other anti-tuberculous drugs and endogenous components do not interfere with measurement of pyrazinamide. The limit of detection of pyrazinamide with this method is 0.2 mg/0.2 ml plasma (CV 8.2%).
Subject(s)
Adult , Antitubercular Agents/blood , Biological Availability , Chromatography, High Pressure Liquid/methods , Humans , Male , Niacinamide/blood , Pyrazinamide/blood , Reproducibility of Results
2.
Article
in English
| IMSEAR
| ID: sea-17549