Effect of two injectable contraceptive drugs on the ovary and endometrium of the adult female albino rat: histological and immuno-histochemical study

This study was carried out to determine the histological and immuno-histochemical changes induced in the ovary and endometrium following injection and stoppage of Depot-Provera and Mesigyna, injectable contraceptive drugs. In addition to determine the relation between the proliferative activity of the endometrium and estrogen receptors content of the ovary. Thirty three adult female albino rats were used in this work, which were divided into three groups; the first group [Ia,Ib,Ic] as control. The second group [IIa and IIb] was injected twice with Depot-Provera once/3 weeks then sacrificed after one day and six weeks respectively from the last injection. The third group [IIIa and IIIb], was injected six times with Mesigyna once/week then sacrificed after one day and two weeks respectively from the last injection. The ovaries and uteri of all rats were taken and stained with H and E stain and immuno-histochemical stains. The H and E stained sections of the ovaries in groups IIa and IIIa revealed atresia and degeneration of the growing follicles with few corpora lutea and some cystic dilatations. In group IIb, they still showed some degenerated follicles but in IIIb, some growing follicles and fresh corpora lutea were observed. The endometria of group IIa revealed few, small and quiescent endometrial glands, which were embedded in a highly vascular and edematous stroma.While group IIIa revealed that the endometrial surface was lined partially by pseudostratified epithelial cells. The endometrial glands appeared tubular dilated and embedded in a moderately dense stroma. In group IIb they still showed small and few endometrial glands embedded in a less edematous stroma. However, in group IIIb, the endometrial histology returned to control pattern. The immunohistochemical [SP1] stained sections of the ovaries of group IIa revealed marked decrease of estrogen receptors content, which increased in group IIb, but still less than control level. On contrary, it was apparently increased in group IIIa and then returned to control level in group IIIb. The [Ki-67] stained sections of the endometria of group IIa revealed reduction in the proliferative activity, which still remained lower than control level in group IIb. However, in group IIIa the proliferative activity was increased but returned to the control level in group IIIb. In conclusion, this study clarified that Mesigyna induced less suppressive effects on the ovary and endometrium with an earlier return of their activity than with Depot-Provera. Also, there was an evident direct relationship between the proliferative activity of the endometrium and estrogen receptors content of the ovary

Anticoncepción en la premenopausia con el inyectable mensual Mesigyna, con especial énfasis en el comportamiento de lípidos séricos y densidad ósea / Contraception in the premenopause with once-a-month injectable mesigyna

Mesigyna administrada a 49 mujeres de entre 38 años de edad y la menopausia, fue comparada durante 2 años con 99 mujeres portadoras de DIU (Nova-T). Se realizó un dosaje completo de lipoproteínas antes del tratamiento y a los 6, 12 y 24 meses de tratamiento. A la mitad de las mujeres se les realizó una densitometría ósea, y una biopsia de endometrio a la mitad del grupo con Mesigyna, antes y al final del primero y segundo año. No se produjo ningún embarazo con Mesigyna mientras que se presentaron 3 embarazos en el grupo con DIU. Hubo una incidencia mayor de anomalías de sangrado en el grupo con Mesigyna, con una tasa de abandono por estas razones del 20 por ciento, contra 4 por ciento para el DIU (p<0,05). La duración de la menstruación, como así también la dismenorrea, fue menor con Mesigyna. No se observaron diferencias en el sangrado intermenstrual ni en la densidad ósea lumbar entre ambos grupos. Ninguna paciente desarrolló hiperplasia endometrial luego del tratamiento, por lo que el riesgo endometrial no se mostró aumentado. Se observó un descenso de colesterol y triglicéridos totales, colesterol HDL, HDL2 e IDL con Mesigyna. Los demás parámetros lipídicos estudiados no mostraron una tendencia definida de variación. El presente trabajo sería el primero utilizando anticoncepción inyectable mensual y su influencia en el comportamiento de los lípidos séricos en mujeres premenopáusicas. Mesigyna fue un agente altamente efectivo y de bajo riesgo para este tipo de usuarias

Effect of noristerat on the development of the retina of the chick embryo

The present work was concerned with the development of the retina of the chick embryo and the effect of noristerat on its development, aiming for the explanation of the possible hazards of the drug which may occur to the fetus, if the pregnant mother is under the treatment with this drug. Morphological and histological studies were done on the retina of 10, 12 and 14-day old chick embryo treated on the 6th day of incubation by a dose of noristerat equivalent to the average human therapeutic dose [0.2 mg/embryo]. Retardation of growth of the retina was observed in all treated embryos as evidenced by reduction of the size of the retina, associated with disappearance of some layers of the retina

Perspectivas de manejo en la hiperplasia endometrial. Informe preliminar / The outlook for the management of endometrial hyperplasia. a preliminary report

Con el propósito de encontrar un tratamiento menos traumático y unificar criterios en nuestro medio en el menejo de la Hiperplasia Endometrial (HE) realizamos una prueba farmacológica en siete mujeres con HE y hemorragia uterina en edad fértil (n=3) y perimenopaútisicas (n=4). Se les prescribió noretindrona (NET) 5 mg/45d. No se observaron efectos colaterales indesables. Las siete pacientes mostraron regresión endometrial, dos de las tres jóvenes lograron embarazo a término y las cuatro perimenopaúsicas no volvieron a menstruar. En estas observaciones preliminares no hubo ningún fenómeno de asociación entre hormonas esteroides gonadales (Estradiol E-2, estrona E-1, Progesterona P-4) con la histología endometrial

Morphologic Effects of Oral Contraceptives (Norinyle) on the Guinea Pig Liver

In view of continuous clinical reports of liver damage following oral contraceptive use, morphologic changes of the liver following the administration of a combined type of oral contraceptive (Norinyle) were studied in guinea pigs by light and electron microscopic examinations. Two interesting changes, one in Kupffer cells and another in the hepatocytes were observed. The Kupffer cells were hyperplastic and enlarged due to the phagocytosis of a large amount of fibrin which were apparently formed intravascularly and were being cleared by Kupffer cells. It is thought that enlarged Kupffer cells and excessive fibrin formation beyond the clearing capacity of Kupffer cells may lead to blockage of sinusoids resulting in a Budd-Chiari like syndrome. The nucleus of the hepatocytes showed mild enlargement and a clear nucleolar segregation which is regarded as one of the ultrastructural evidences of chemical carcinogenesis. Therefore, it is speculated that this change may be related to tumorigenesis in the liver following oral contraceptive administration. Other changes consisted of ischemic necrosis and fatty changes in occasional animals, alteration of mitochondrial configuration and hyperplasia of smooth endoplasmic reticulum in hepatocytes.

study of injectable contraceptives

This is a study of the acceptability, side effects and value of two injectable contraceptives: Lynoestrenol phenyl propionante and Norethisterone enanthale in 181 fertile women. The first preparation was injected monthly in 65 women for 442 cycles and the second was given every 12 weeks in 89 women, for a total of 297 injections. Effects on menstruation and lactation were observed and the side effects were recorded. Their contraceptive value in 68.9 woman years was studied. The second preparation was better as a contraceptive but caused much more frequent and serious side-effects than the first