Seroprevalence of three influenza A viruses (H1N1, H3N2, and H3N8) in pet dogs presented to a veterinary hospital in Ohio

The prevalence of canine H3N8 influenza and human H1N1 and H3N2 influenza in dogs in Ohio was estimated by conducting serologic tests on 1,082 canine serum samples. In addition, risk factors, such as health status and age were examined. The prevalences of human H1N1, H3N2, and canine H3N8 influenzas were 4.0%, 2.4%, and 2.3%, respectively. Two samples were seropositive for two subtypes (H1N1 and H3N2; H1N1 and canine influenza virus [CIV] H3N8). Compared to healthy dogs, dogs with respiratory signs were 5.795 times more likely to be seropositive against H1N1 virus (p = 0.042). The prevalence of human flu infection increased with dog age and varied by serum collection month. The commercial enzyme-linked immunosorbent assay used in this study did not detect nucleoprotein-specific antibodies from many hemagglutination inhibition positive sera, which indicates a need for the development and validation of rapid tests for influenza screening in canine populations. In summary, we observed low exposure of dogs to CIV and human influenza viruses in Ohio but identified potential risk factors for consideration in future investigations. Our findings support the need for establishment of reliable diagnostic standards for serologic detection of influenza infection in canine species.

Effects of an integrated geriatric group balance class within an entry-level Doctorate of Physical Therapy program on students' perceptions of geriatrics and geriatric education in the United States / 보건의료교육평가

This study was aimed at determining the effect of an integrated group balance class for community-dwelling older adults within entry-level physical therapist coursework on student perceptions of geriatric physical therapy and geriatric physical therapy education. Twenty-nine Doctor of Physical Therapy (DPT) students, 21–33 years old, in their second year of coursework in 2012, participated in an integrated clinical experience with exposure to geriatric patients at an outpatient facility at the Louis Stokes Cleveland Department of Veterans Affairs Medical Center in Akron, Ohio, USA. Student perceptions were collected before and after participation in the 8-week balance class. The Wilcoxon sign-ranked test was used to identify differences in perceptions after participation in the group balance class. Cohen's d-values were calculated to measure the size of the pre-participation to post-participation effect for each measure. At the conclusion of the group class, the DPT students demonstrated an increase in positive perceptions of geriatric physical therapy in 8 measures, with small effect sizes (d=0.15–0.30). Two perceptions of geriatric physical therapy demonstrated a significant positive increase (P<0.05) with moderate effect sizes (d=0.47 and d=0.50). The students’ perceptions of geriatric education in the curriculum demonstrated a large positive effect for quality (d=1.68) and enjoyment (d=1.96). Positive changes were found in most of the perceptions of geriatrics and geriatric education after participation, suggesting that integrated clinical experiences with geriatric patients are an effective way to positively influence perceptions of physical therapist practice with older adults.

Appendiceal Neuroendocrine, Goblet and Signet-Ring Cell Tumors: A Spectrum of Diseases with Different Patterns of Presentation and Outcome / Journal of the Korean Cancer Association, 대한암학회지

PURPOSE: Appendiceal tumors are a heterogeneous group of diseases that include typical neuroendocrine tumors (TNET), goblet cell carcinoids (GCC), and atypical GCC. Atypical GCC are classified into signet-ring cell cancers (SRCC) and poorly differentiated appendiceal adenocarcinoids. The prognosis and management of these diseases is unclear because there are no prospective studies. The aim of this study is to assess the characteristics and outcome of appendiceal TNET, GCC, and SRCC patients. MATERIALS AND METHODS: Appendiceal TNET, GCC, and SRCC patients diagnosed between 1973 and 2011 were identified in the Surveillance Epidemiology and End Results (SEER) database. Demographics, type of surgery, and clinicopathologic characteristics were collected. Survival functions were estimated by the Kaplan-Meier method, and log-rank test was used to assess the difference in overall survival (OS) among the three histologies. RESULTS: The SEER database yielded 1,021 TNET patients, 1,582 with GCC, and 534 SRCC patients. TNET presented at a younger age (p < 0.001). Patients with SRCC presented with advanced stage disease (p < 0.001). The median OS (mOS) for GCC and TNET patients was not reached; mOS for SRCC was 24 months. Multivariate analysis stratified for stage revealed significantly longer survival for TNET and GCC than SRCC (p < 0.001). CONCLUSION: This is the largest report to date for appendiceal neuroendocrine tumor patients, suggesting a spectrum of diseases with different characteristics and outcomes. In this report, we present a treatment approach for this complex spectrum of disease, based on the experience of Ohio State and Emory Universities investigators.

Faculty-supervised measurements of the face and of mandibular movements on young adults

PURPOSE: The purpose of this study was to determine the average facial proportions and mandibular movement capacity of 316 first-year dental students who carefully recorded them on each other. MATERIALS AND METHODS: This early exacting clinical experience was closely supervised by the authors in Columbus, Ohio during 1969-70. Five vertical and six horizontal distances were measured on each subject's face. An ala-tragus line and an occlusal line were drawn on the left side of the face to determine if these two lines were parallel. Measurements of mandibular movements involved maximum normal and hinge opening at the incisors and maximum amounts of right, left lateral and protrusive excursions of the mandible. RESULTS: The ala width and distance between the tips of upper right and left canine cusps averaged (35.2 mm and 34.8 mm) but with very large individual variations. The distance between ala to occlusal plane lines was 29.9 mm at the tragus and 31.3 mm near the ala. The angle between orbitale and ala-tragus averaged 13.6 degrees. CONCLUSION: The upper lip length was the most variable and the distance between the pupils was the most stable of the eleven facial measurements. The ala-tragus line and the occlusal plane lines were for all practical purposes parallel. Maximum jaw opening averaged 51.2 mm which was 3.0 times larger than maximal hinge opening of 17.2 mm. The maximum right plus left side jaw excursions (9.2 and 9.4 mm) totaled 18.6 mm, 2.3 times more than the 8.0 mm mean maximum forward protrusion.

Diminution of Secondary Injury after Administration of Pharmacologic Agents in Acute Spinal Cord Injury Rat Model: Comparison of Statins, Erythropoietin and Polyethylene Glycol

STUDY DESIGN: An experimental animal study. OBJECTIVES: To evaluate and compare the neuroprotective effect of statins, erythropoietin and polyethylene glycol (PEG) after spinal cord injury (SCI). SUMMARY OF LITERATURE REVIEW: There are few comparative studies of pharmacological agents for acute SCI. MATERIALS AND METHODS: Forty Sprague Dawley (SD) rats had a spinal cord injury at T9/10 using an Ohio State University (OSU) impactor. The animals were randomized to receive one of the following; simvastatin, erythropoietin, PEG or saline. A behavioral outcome assessment was performed on days 2, 4 and 7, and then every week using the Basso, Bresnahan, and Beattie (BBB) score and subscore. The animals were sacrificed at the end of 6 weeks and histologic assessment was performed to measure the areas of white and gray matter. RESULTS: For the animals treated with simvastatin, erythropoietin, PEG and saline, the mean BBB scores at 6 weeks post-injury were 13.2+/-0.1, 11.7+/-0.4, 13.3+/-0.3, and 11.4+/-0.2, and the BBB subscores were 9.2+/-1.1, 5.0+/-1.3, 9.1+/-1.1, 4.4+/-1.2, respectively. The BBB scores and BBB subscores were significantly higher in simvastain and PEG-treated animals (p<0.05). The areas of white matter at the lesion epicenter were 0.78+/-0.05mm2, 0.46+/-0.04 mm2, 0.68+/-0.15 mm2, and 0.41+/-0.04mm2 in the simvastatin, erythropoietin, PEG and saline groups, respectively. The simvastatin and PEG-treated animals showed increased sparing of the white matter at the injury epicenter and at 0.2mm rostral and 0.4mm caudal(p<0.05). CONCLUSION: Simvastatin and polyethylene glycol administration showed diminished secondary injury after SCI in rats. In addition, they showed almost the same efficacy. However, erythropoietin did not show neuroprotective effect.

The Neuroprotective Effect of Combination Therapy of Polyethylene Glycol and Magnesium Sulfate in Acute Spinal Cord Injury / 대한정형외과학회잡지

PURPOSE: To evaluate the neuroprotective effect of combination therapy of polyethylene glycol (PEG) and magnesium sulfate (MgSO4) after a spinal cord injury. MATERIALS AND METHODS: Twenty Sprague Dawley male rats (300-350 gm) had a spinal cord injury after T9/10 laminectomy using an Ohio State University (OSU) impactor under intraperitoneal anesthesia. The animals were randomized to receive either PEG (1 g/kg)+MgSO4 (300 mg/kg) or saline (2 ml) via carotid vein after 2 hours of injury and then every 6 hours for 5 times. The behavioral outcome assessments were performed on days 2, 4 and 7, and then every week using the Basso, Bresnahan, and Beattie (BBB) score and subscore. The animals also underwent sensory threshold testing using a von Frey monofilament device and gait analysis with Catwalk program before and 6 weeks after cord injury. The animals were sacrificed at the end of 6 weeks and histologic assessment was performed to measure the areas of white and gray matter. RESULTS: For the animals treated with PEG+MgSO4 and saline, the mean BBB scores at 6 weeks post-injury were 13.3+/-0.3, 11.4+/-0.2 and the BBB subscores were 9.1+/-1.1, 4.4+/-1.2 respectively (p<0.05). No significant differences were found in sensory testing and gait analysis between the two groups. Histologic assessment revealed no significant difference in gray matter sparing but the areas of white matter at the lesion epicenter were 0.68+/-0.2, 0.41+/-0.04 mm2 in the PEG+MgSO4 and saline groups respectively, which indicated significant sparing of white matter in PEG+MgSO4 group (p<0.05). CONCLUSION: The combination therapy of polyethylene glycol and magnesium sulfate improved the motor function and showed significant histological sparing of the spinal cord after an acute spinal cord injury in rats.

The Relationship between Brachial Ankle Pulse Wave Velocity andAtherosclerotic Risk Factors

PURPOSE: Aging and atherosclerotic changes enhance the stiffness of the arterial wall, and the pulse wave travels faster in stiffer vessel. Measurement of the brachial ankle pulse wave velocity (baPWV) is a non-invasive method for evaluating the stiffness of the vessel wall. We investigated the relation between the baPWV and risk factors for atherosclerosis. METHOD: We studied 180 subjects (38 male and 142 female; mean age 46 years, range 24 to 76 years). The instrument used for evaluating the baPWV was a Vasoguard (VIASYS Healthcare, Dublin, Ohio, USA), and measurements were performed in the right arm and in both ankles. Data were analyzed using Pearson correlation, t-test, and multiple regression analysis. Multiple regression analysis was performed for age, sex, smoking, hypertension, height, weight, body mass index (BMI), total cholesterol, LDL, HDL, TG, and HbA1c. RESULT: Right and left baPWVs were significantly increased (P<0.05) in subjects with the following risks: older age, high body weight, high BMI, high total cholesterol, high LDL, high TG, high HbA1c, and low HDL. Right and left baPWVs were also significantly increased (P<0.05) in male subjects with a history of smoking and hypertension. Multiple regression analysis showed that age, sex, and LDL were independent determinants of the right and left baPWVs. CONCLUSION: This study provides evidence that increased age, male gender, and high serum LDL levels are risk factors that contribute to arterial stiffness. Measurement of the baPWV may be a useful method for evaluating vascular status.

Neuroprotection for Acute Spinal Cord Injury: Comparison of Simvastatin and Atorvastatin / 대한정형외과학회잡지

PURPOSE: To evaluate the neuroprotective effect of statins after a spinal cord injury. MATERIALS AND METHODS: Twenty four Sprague Dawley rats had a spinal cord injury at T9/10 using an Ohio State University (OSU) impactor. The animals were randomized to receive either simvastatin, atorvastatin, or saline with oral gavage everyday for 7 days. A behavioral outcome assessment was performed on days 2, 4 and 7, and then every week using the Basso, Bresnahan, and Beattie (BBB) score and subscore. The animals also underwent sensory threshold testing using a von Frey monofilament device. The animals were sacrificed at the end of 6 weeks and a spinal cord specimen was harvested. Histology and immunohistochemistry were performed to measure the areas of white and gray matter, and the sparing of oligodenrocytes. RESULTS: For the animals treated with simvastatin, atorvastatin and saline, the mean BBB scores at 6 weeks post-injury was 13.2+/-0.1, 11.8+/-0.5, and 11.3+/-0.2 and the BBB subscores were 9.2+/-1.1, 4.8+/-1.8 and 4.4+/-1.4 respectively (p<0.05). The areas of white matter at the lesion epicenter were 0.78+/-0.05, 0.5+/-0.18 and 0.41+/-0.03 mm2 in the simvastatin, atorvastatin and saline groups respectively, and the number of spared oligodendrocytes was significantly higher in the simvastatin treated animals (p<0.05). CONCLUSION: The simvastatin treatment improved the behavior and histological sparing of the spinal cord after an acute spinal cord injury in rats.

Epidemiology of Respiratory Viral Infection in 2004-2006 / 대한진단검사의학회지

BACKGROUND: The information on the incidence, seasonal variation and clinical pattern of respiratory virus infections is very important for clinicians in managing their patients. This study was aimed to define the epidemiology of respiratory viral pathogens in Seoul and the neighboring areas from March 2004 to February 2006. METHODS: A total of 6,533 specimens were cultured for respiratory viruses during the study period. Madin-Darby canine kidney (MDCK), LLC-MK2, and HEp-2 cells, or R-mix cells (Diagnostic Hybrids Inc., Athens, Ohio, USA) were used for culture. Influenza virus types A & B (Inf A & B), parainfluenza virus (PIV), respiratory syncytial virus (RSV), and adenovirus (ADV) were identified by indirect immuno-fluorescent staining. Medical records of the patients with positive virus cultures were reviewed retrospectively. RESULTS: One or more viral agents were isolated from 1682 specimens (25.7%). The pathogens identified were RSV 37.2%, ADV 19.9%, Inf A 18.9%, PIV 17.5% and Inf B 6.4%. The most frequent pathogen of pneumonia and acute bronchiolitis was RSV and that of croup was PIV. Upper respiratory tract infections were more prevalent in adults and the most frequently caused by influenza virus. Influenza virus itself was more frequently isolated in children less than six years old, which was different from previous reports. Influenza virus was mostly isolated in the winter and spring, while RSV was usually isolated from early fall with a peak incidence in the winter. Inf A and RSV showed a dampening effect on the occurrence of other viruses during their major epidemic. PIV was mostly detected in the spring and summer. ADV was isolated throughout the whole year. CONCLUSIONS: The epidemiological characteristics of respiratory virus infections in Seoul and the neighboring areas in 2004-2006, were similar to the findings of previous reports except for some minor changes. These findings could be useful to clinicians in managing their patients.

A Case of Blastomycosis after Traveling around Non-Endemic Area / 결핵및호흡기질환

Blastomycosis is a systemic pyogranulomatous disease that is caused by a thermally dimorphic fungus, Blastomyces dermatitidis. it's the disease is endemic in the south-eastern and south central states of the USA, which border the Mississippi and Ohio Rivers, the mid-western states and Canadian provinces bordering the Great Lakes as well as in a small area of New York and Canada adjacent to the St. Lawrence River.1 We encountered a case of blastomycosis, representing as a pulmonary manifestation after traveling around a non-endemic area and report it with a brief review of the relevant literature.

A Comparative Study for Mesopic Contrast Sensitivity between Keratectomy(PRK) and Laser in Situ Keratomileusis(LASIK)

PURPOSE: To compare the effect on visual performance of photorefractive keratectomy (PRK) and laser in situ keratomileusis (LASIK) with mesopic contrast sensitivity using CSV-1000S in excimer laser refractive surgery using the broad beam type and manual microkeratome. METHODS: Postoperative visual performance for 40 eyes undergoing PRK and 40 eyes undergoing LASIK was compared with mesopic contrast sensitivity at 1, 3, and 6 months postoperatively using the CSV 1000 (VectorVision, Ohio, USA). Eyes were divided into two groups: less than 6.0 D and over 6.25 D. Mesopic contrast sensitivity was performed with the patient's uncorrected-visual acuity of 20/20 or better under photopic condition. RESULTS: Under mesopic conditions a significant decrease in contrast sensitivity for the postoperative PRK and LASIK group was obtained at all spatial frequencies compared to the preoperative baseline (p0.05). CONCLUSIONS: In the broad beam excimer laser, in cases of myopia with below 6.0 D, PRK seemed to have a more significant effect on mesopic contrast sensitivity than LASIK using manual microkeratome at postoperative 6 months.

A comparison of density of Insight and Ektaspeed plus dental x-ray films using automatic and manual processing / 대한구강악안면방사선학회지

PURPOSE: To compare the film density of Insight dental X-ray film (Eastman Kodak Co., Rochester, NY, USA) with that of Ektaspeed Plus film (Eastman Kodak) under manual and automatic processing conditions. MATERIALS AND METHODS: Insight and Ektaspeed Plus films were exposed at three different exposure conditions with an aluminum step wedge on the films under the three different exposure times. The exposed films were processed by both manual and automatic ways. The Base plus fog density and the optical density made by exposing step wedge were calculated using a digital densitometer (model 07-443, Victoreen Inc, Cleveland, Ohio, USA). The optical densities of the Insight and Ektaspeed film versus thickness of alumimun wedge at the same exposure time were plotted on the graphs. Statistical analyses were applied for comparing the optical densities of the two films. RESULTS: The film density of both Insight films and Ektaspeed Plus films under automatic processing condition was significantly higher over the manual processing. The film density of Insight films was significantly higher than that of Ektaspeed Plus films on both automatic and manual processing conditions. CONCLUSION: The radiation exposure time can be reduced when using Insight over Ektaspeed Plus film. To take the full advantage of reducing exposure time, Insight film should be processed automatically.

Development and Performance Test of Preamplifier and Amplifier for Gamma Probe / 대한핵의학회잡지

PURPOSE: Preamplifier and amplifier are very important parts for developing a portable counting or imaging gamma probe. They can be used for analyzing pulses containing energy and position information for the emitted radiations. The commercial Nuclear Instrument Modules (NIMs) can be used for processing these pulses. However, it may be improper to use NIMs in developing a portable gamma probe, because of its size and high price. The purpose of this study was to develop both preamplifier and amplifier and measure their performance characteristics. MATERIALS and Methodes: The preamplifier and amplifier were designed as a charge sensitive device and a capacitor resistor-rsistor capacitor (CR-RC) electronic circuit, respectively, and they were mounted on a print circuit board (PCB). We acquired and analyzed energy spectra for Tc-99m and Cs-137 using both PCB and NIMs. Multichannel analyzer (Accuspec/A, Caberra Industries Inc., Meriden Connecticut, U.S.A) and scintillation detectors (EP-047 (Bicron Saint-Gobain/Norton Industrial EP-047 (Ceramics Co., Ohio, U.S.A) with 2"x2" NaI (T1) crystal and R1535 (Hamamatsu Photonics K.K., Electron Tube Center, Shizuoka-ken, Japan) with 1"x1"NaI (T1) crystal) were used for acquiring the energy spectra. RESULTS: Using PCB, energy resolutions or EP-047 detectors for Tc-99m and Cs-137 were 12.92% and 5.01%, respectively, whereas R1535 showed 13.75% and 5.19% of energy resolution. Using the NIM devices, energy resolutions of EP-047 detector for Tc-99m and Cs-137 were measured as 14.6% and 7.58%, respectively. However, reliable energy spectrum of R1535 detector could not be acquired, since its photomultiplier tube (PMT) requires a specific type of preamplifier. CONCLUSION: We developed a special preamplifier and amplifier suitable for a small sized gamma probe that showed good energy resolutions independent of PMT types. The RESULTS indicate that the PCB can be used in developing both counting and imaging gamma probe.

End - tidal Carbon Dioxide Measurements in the Pediatric Patients : A Comparison of End - tidal PCO2 Sampled from the Proximal and Distal Ends of Pediatric Uncuffed Tracheal Tube / 대한마취과학회지

To determine the accuracy of end-tidal PCO2(PetCO2) measurement analysed with Ohmeda 5210 capnometer in the pediatric patients whose jung were ventilated with Ohio infant Circle Absorber and Ohio Ventilatior 7200, we compared PetCO2, measurements sampled from the proximal(PetCO2-p) and distal(PetCO2-d) ends of the tracheal tube to arterial PCO2(PaCO2) in 22 healthy pediatric patients between 6.8 to 18.5kg. The results were as follow: 1) Proximal PetCO2 and distal PetCO2 correlated with PaCO2(r=0.76%, SEE=2.79, r=0.70:SEE=3.01, respectively)(p<0.05). 2)Arterial to end-tidal PCO2 difference(delta(a-et)PCO2) was significantyly greater with distal(3.53+/-4.68 mmhg) than proximal(0.35+/-2.72 mmhg) sampling(p<0.05). 3) In subgroup of patients whose proximal to distal concentration of nitrous oxide difference was more than 2%, the delta(a-et)PCO2 using distal sampling(7.90+/-5.15 mmhg) was also significantly greater then it was using proximal sampling(-1.73+/-2.39 mmhg)(p<0.05).. We conclude that distal estimates of PetCO2-P can not provide accepatbale estimate of PaCO2 in healthy pediatric patients who are intubated with pediatric uncuffed tracheal tube.

SARACAP Monitoring of Anesthesia Machines during Anesthesia / 대한마취과학회지

With recent rise in medico-legal problems, there is also an increase in number of problems involved with anesthesia. Major causes of anesthetic accident are malfunctioning anesthetic machine and inappropriate gas flow and concentration of anesthetic gas, resulting from malfunctioning ventilatory circuit. Therefore, anesthesiologist needs an apparatus to determine the appropriateness of ventilation and a monitoring system to measure a concentration of inspired O2 and inhalation anesthetics. To prevent circulatory and respiratory abnormalities and to promote the safty during anesthesia, we used the SARA monitoring system, as an indicator of safty measure. We monitored the anesthesia machines (n=17), using in operating room in Shinchon Severance hospital, by using the SARA monitoring system which was connected to the distal portion of endotracheal tube. We intended to analysis the relationships between anesthetic machines, vaporizers, ventilators and parameters measured form each machine, in clinical use. The results were as follows; 1) Average used year of anesthesia machines was 7.7 years, that of vaporizers was 7.6 years and that of ventilator's was 4.0 years. 2) When vaporizers dial was fixed at enflurane 2.0%, average measured-FiEnf was 2.02+/-0.22%, equal to dial setting and average FiEnf was 1.70+/-0.25%, usually lower than dial setting. 3) In some anesthesia machines, CO2 rebreathing occured always during ansthesia and FiEnf & FeEnf were decreased in case of CO2 rebreathing. 4) FiEnf by vaporizers was decreased as following orders, Draeger, Ohio and Cyprene.

A Economic Device of Gas Evacuator designed with Angle Stop Valve / 대한마취과학회지

Recent studies suggest possible deleterious effects of chronic exposure to certain common anesthetic agents. A simple and economic gas evacuator was designed with an angle stop valve of a water pipc system which was easily purchased from a hardware store. The device was installed as follows: 1) The pop-off valve assembly of an Ohio Unitrol anesthesia machine was removed and replaced with atail-piece of an angle stop valve with a new screw which was made to fit in place. 2) The disk of the angle stop valve was fixed in place with adhesive cement. 3) The tail nut and tail packing of the angle stop valve were replaced with the same caliver long vinyl hose, which eliminated overflow anesthetic gases from the anesthesia machine air vent system of the operating room. This device is inexpensive and simple to operate and is being used successfully in routine clinical practice.

A Clinical Study of Anesthesia for Tracheoesophageal Fistula with Esophageal Atresia / 대한마취과학회지

Two cases of anesthetic experience with tracheoesophageal fietula with esophageal atresia have-been reported and the literature reviewed. On the fifth day of life, anesthesia was induced and maintained with nitrous oxide-oxygen- halothane using Ohio infant circle absorber, preceded by awake endotracheal intubation with the aid of intramuscular injection of succinylcholine chloride following preliminary oxygenation. Gastro-stomy was performed prior to surgery. Tracheoesophageal fistula was divided and the esophagus anastomosed. In the first case surgical manipulation of the lungs and trachea caused complete airway obstruction and momentary cardiac arrest, which was successfully treated with I.V. calcium gluconate and sodium bicarbonate. Both infants made otherwise uneventful recoveries. The importance of preliminary gastrostomy, atraumatic intubation, maintenance of adequate ventilation and circulation, frequent suctioning of airway secretions, and of treating pulmonary complications have been stressed.

Comparative Evaluation of Cyclopropane and Fluothane Anesthesia During Hemorrhagie Shock in Dogs / 대한마취과학회지

In order to comparatively evaluate the effects of cyclopropane and fluothane upon mean arterial pressure. cardiac output and splanchnic blood flow during hemorrhagic shock a Iaboratory investigation was conducted in the following three successive phases in two groups: Group I (cyclopropane):1l. Observation 60 minutes following Nembutal I .V. injection 2. Observation 30 minutes following acute hemorrhage 3. Observation 30 minutes following cyclopropane anesthesia Group II (fluothane): 1. Observation 60 minutes following Nembutal I .V. injection 2. Observation 30 minutes following acute hemorrhage 3. Observation 30 minutes following fluothane anesthesia Nine mongrel dogs weighing 11.2±1. 2kg composed each group. Immediately following induction of anesthesia with intravenous pentobarbital (Nembutal), all dogs were endotracheally intubated with the aid of intravenous succinylcholine chloride. Spontaneous respiration was allowed during pentobarbital anesthesia, but ventilation was controlled with Ohio Anesthesia Ventilator during cyclopropane or fluothane anesthesia. In order to insure the adepuacy of ventilation, arterial blood samples were drawn at appropriate intervals for analysis of pH and Pco2 by Beckman Blood Gas Measurement System. Ventilation was adjusted so that the pH and Pco were maintained in the range of 7.30~7.35 and 30~35 mmHg. A heating pad was used to maintain the esophageal temperature at the normal leveal (±0.5 ℃). The internal carotid artery and femoral vein were cannulated. Carotid arterial pressure was measured by sensing with a Statham transducer. Lead II of the electrocardiograph and arterial presaure were continuously monitored end recorded on the Beckman RM 4-Channel Monitor/Recorder. The abdomen was opened and the portal vein and common hepatic artery were cannulated with IVM FT-P Blood Flow Transducer and the blood flow was recorded on EMF-120 Electromagnetic Blood Flowmeter. Bleeding amounted to approximately 2per cent of the body weight. Cardiac output was measured by the direct Fick method. Cyclopropane was administered in the concentration of 10 per cent(100cc/min cyclopropane-900cc/min oxygen) and fluothane-was administered in 1 per cent concentration from Mark II Fluotec vaporizer. Both anesthetics were delivered in semiclosed circle carbon dioxide absorption system. The results are summarized in Tables 1~2. In brief both cyclopropane and fluothane caused a further reduction in splanchnic blood flow by 14 per cent and 21 per cent respectively during hemorrhagic hypotension. Mean arterial pressure rose 24 per cent and cardiac output increased 17 per cent by cyclopropane, whereas mean arterial pressure fell 25 per eent and cardiac output decreased 24 per eent by fluothane. Furthermore, five out of nine dogs inhaled fluothane did not survive despite retransfusion after the experiment. All changes were conaistent and atatistically significant (P<0. 001). The reults suggest that for patients with hypovolemie hypotension without blood replacement cyclo- propane would be the inhalation anesthetic of choice, fluothane might be detrimental in such circum-stances, and that the choice of fluothane ahould be reserved for normovolemic vasnconatrictive states.