[Anti-Mullerian hormone as a predictor of IVF treatment]

Anti-Mullerian Hormone [AMH] is secreted from granulosa cells of growing follicles and is a useful marker of ovarian reserve. Fertility in women is determined by the quality and quantity of follicle pool and ovarian follicular reserve positively correlates with AMH. In this study we aimed to determine if AMH can predict ovarian response in IVF treatments. In this retrospective observational study undertaken in Mehr Institute during 2010 to 2011, we studied the medical records of 101 patients and recorded the concentrations of AMH, day two or three FSH, LH, basal estradiol [E2], E2 on day of HCG administration and the number of metaphase II oocytes. Having undergone ovarian hyperstimulation, the women were divided into three groups: poor responders [retrieved oocytes /= 16]. Overall, 20% of patients were defined as poor responders, 71% as average responders and 10% as high responders. There were significant differences in the concentration of AMH, E2 on day of HCG administration, FSH, the number of metaphase II oocytes and age between the three groups. MII oocyte count correlated positively with AMH [r=0.487], basal E2 [r=0.275] and LH [r=0.07] but it did negatively with FSH [r=-0.26] and age [r=-0.04]. The areas under curve for AMH, FSH, LH, E2 and age were 0.799, 0.32, 0.429, 0.558 and 0.304, respectively. We determined the 0.85 ng/ml AMH concentration as the cut-off point with 71% specificity and 79% sensitivity for the prediction of poor responders. anti-mullerian hormone is an appropriate predicator of ovarian response following induction of ovulation

Efeito da frequência de amamentação sobre a taxa de ovulação após tratamento com desmame temporário + GnRH, sobre a incidência de luteólise prematura no primeiro ciclo estral pós-parto e sobre o desempenho da progênie de vacas Nelore / Effect of suckling frequency on ovulation rate after temporary weaning + GnRH treatment, incidence of premature luteolysis in the first postpartum estrous cycle, and performance of calves from Nelore cows

Avaliou-se o efeito da restrição na frequência de amamentação sobre o diâmetro folicular no dia 0 (DFOL), sobre a taxa de ovulação (TO), e sobre a incidência de luteólise prematura no primeiro ciclo estral pós-parto (ILP) de vacas Nelore multíparas, em anestro, submetidas à amamentação ad libitum (controle; n= 115) ou amamentação uma vez ao dia (restrito; n= 109), entre os dias -14 e 9 do experimento, e estudou-se o efeito desses tratamentos sobre o peso à desmama da progênie dessas vacas. Induziu-se ovulação com remoção de bezerros entre os dias -2 e 0 e aplicação de 100μg de GnRH no dia 0. Somente animais que ovularam foram mantidos no experimento (n= 125). A ocorrência de luteólise prematura foi avaliada por meio da dosagem da concentração sérica de progesterona nos dias 5 e 9. A TO não foi influenciada pelos tratamentos (55,8 por cento; P>0,1), e as vacas do tratamento restrito apresentaram maior DFOL (10,90±0,26 vs. 10,18±0,21mm; P<0,05) e menor ILP (21,4 por cento vs. 43,5 por cento; P<0,05). Os bezerros do tratamento controle foram mais pesados (162,32±2,08 vs. 155,91±4,12kg; P<0,05). Conclui-se que a restrição na frequência de amamentação em vacas Nelore reduz a ILP, porém com possível efeito negativo no desenvolvimento dos bezerros.

The effects of restricted suckling on follicular diameter at day 0 (FDDO), ovulation rate (OR), and incidence of premature luteolysis in the first post partum estrous cycle (PLI) of Nelore cows and the effects on weight at weaning (WW) of progenie of these cows were evaluated. Multiparous anestrous postpartum Nelore cows were submitted to ad libitum suckling (control; n= 115) or once-a-day suckling (restricted; n= 109) from days -14 to 9 of the experiment. For both treatments, a temporary calf removal was performed from days -2 to 0, and on day 0 cows received 100μg of GnRH. Only animals that ovulated after GnRH treatment were used in the experiment (n= 125). The occurrence of premature luteolysis was evaluated by dosage of serum progesterone concentrations on days 5th and 9th. The OR was not affected by treatments (55.8 percent; P>0.1), but cows from restricted treatment had larger FDDO at time of GnRH treatment (10.90±0.26 vs. 10.18±0.21mm; P<0.05) and lower PLI (21.4 percent vs. 43.5 percent; P<0.05) than cows from control treatment. Calves from control treatment had higher WW than calves from restricted treatment (162.32±2.08 vs. 155.91±4.12kg; P<0.05). These results indicate that the restriction in frequency of suckling in Nelore cows may decrease the incidence of premature luteolysis in the first postpartum estrous cycle; however, with possible negative effects on calves development.