ABSTRACT
The resazurin test (REZ TEST) was performed on semen samples obtained from 75 untreated subfertile and 10 pregnancy confirmed fertile males. The results were compared with other sperm parameters to assess the quality of semen. The highest correlation of REZ grade was with motile sperm (r = 0.90, P < 0.001), sperm viability (r = 0.80, P < 0.001) and sperm concentration (r = 0.71, P < 0.001). The REZ grade indicated significant difference in the rate of positive tests between samples from fertile males and patients exhibiting abnormal semen parameters. The RES TEST had a positive predictive value of 93.75% for a progressives motile sperm concentration of > 20 x 106/ml and a negative predictive power of 88.7% for the same variable at a criterion value of 10 x 106/ml. It is suggested that REZ TEST can be performed with a relatively small volume of semen, it is easy to perform and requires no technical equipments.
Subject(s)
Adult , Case-Control Studies , Cell Survival , Diagnostic Errors , Female , Humans , Indicators and Reagents , Infertility, Male/diagnosis , Male , Oxazines/diagnosis , Oxidation-Reduction , Pregnancy , Semen/cytology , Sperm Count , Sperm Motility , Spermatozoa/metabolism , XanthenesABSTRACT
85 liver samples were stained with H & E and orcein. Of these 40 were from medicolegal autopsies, 30 from preserved specimens of medical college museums and 15 wedge biopsies. Three of the five cirrhotic museum specimens showed HBsAg by orcein. The remaining cases including three cirrhosis and two HCC were negative for HBsAg. HBsAg was found to be more frequently associated with cirrhosis. The autopsy samples were histologically normal and HBsAg was not seen. Old museum specimens did not loose their orcein positivity despite preservation in formalin for a long time.