An Analysis on the Amount of Excreted Pancreatic Juice after Pancreatoduodenectomy / 대한소화기학회지

BACKGROUND/AIMS: There has been lack of data on the secretion of the pancreatic juice after pancreatoduodenectomy. Thus, surgeons have difficulties in predicting the clinical sequences of pancreaticojejunostomy leakage or other pancreatic surgery. METHODS: This study employed 15 patients who underwent pylorus-preserving pancreatoduodenectomy (PPPD) with total external drainage of the pancreatic duct from January 2000 to June 2001. RESULTS: These patients were divided into the normal pancreas group (n=11) and chronic pancreatitis- accompanied group (n=4). Excretion of pancreatic juice was suppressed in a few days after PPPD. Then, it gradually increased and became constant at the postoperative day 4. The amount of excreted pancreatic juice in the normal pancreas group was 229 +/- 99, 85~385 (mean, range) mL/day at the postoperative day 7 before the initiation of oral intake, and was increased to 314 +/- 222, 110~910 (mean, range) mL/day at the 7th day after the initiation of food intake. The usage of octreotide during early fasting period had no influence on the excretion of the pancreatic juice in the normal pancreas group. CONCLUSIONS: We observed the real amount of secreted pancreatic juice after PPPD. These data may be helpful to interpret and manage the postoperative conditions such as pancreatic leakage or pancreatic exocrine insufficiency.

Analysis of human pancreatic juice in tests of pancreatic function

No abstract available.

Studies on the mechanism of biotin uptake by brusch-border membrane vesicles of hamster enterocytes

Several studies on biotin intestinal transport in the hamster have shown a biotin-specific carrier, but there are conflicting reports on whether it is transported actively, or by facilitated diffusion and on its Na+ dependence. We have studied it for the first time using brush-border membrane vesicles (BBMV), with concentrations in a more physiological (nanomolar)range and found an overshoot component, evidencing a carrier-mediated active process, driving the vitamin against a concentration gradient. Uptake was substantially reduced when potassium substituted for sodium. When the vesicles were treated with trypsin, Na+-dependent uptake was markedly reduced and the overshoot phenomenon was abolished, providing additional evidence for the carrier-mediated trasnport. The amount of uptake in a K+ gradient was considered due to passive diffusion and was about 30 percent of that observed in a Na+ gradient. A similar amount was observed when trypsinized vesicle were incubated in this latter gradient. Our results indicate that in the hamster's brush border intestinal epithelium, Na+-dependent active transport is the most important component in the intestinal uptake of biotin at nanomolar concentrations

Estudio in vivo de la secreción de calcio por el páncreas exocrino del raton: relación con la secreción enzimática / In vivo study of the calcium secretion by the exocrine pancreas of mice: relationship with enzymatic secretion

La secreción de calcio y su relación con la secreción de quimiotripsina (QT) por el páncreas del ratón, in vivo, fueron determinadas en jugo pancreático puro colectado bajo tres condiciones de estimulación: secretina 32 mU/gm; secretina 32 mU/mg más colecistocinina 16 mU/gm y secretina 32 mU/gm más betanecol 0.2 ug/gm. En las muestras obtenidas se determinó actividad de quimotripsina y calcio secretado en 30 minutos luego de la estimulación. La secreción de calcio y QT fue mayor en ratones tratados con secretina más CCK y secretina más betanecol. En estos dos últimos grupos, entre ambos parámetros se estableció una correlación positiva. Las rectas obtenidas no fueron diferentes de una recta única y la extrapolación al origen indica que existe calcio en el jugo pancreático aún ausencia de secreción enzimática. Los resultados obtenidos concuerdan con la existencia de al menos dos mecanismos diferentes de secreción de calcio por el páncreas exocrino del ratón

Elimination of Basic Fuchsin and Other Dyes from the Pancreas

Twenty dyes which previously have been claimed to be excreted in pancreatic juice were reinvestigated to determine to what extent they could be eliminated through the pancreas. Exogenous secretin or cholecysto-kinin-pancreozymin(CCK-PZ) stimuli were used in dogs which had been given intravenous dye solutions at the rate of 1mg/min. In this experiment among the twenty dyes, only six were found to be eliminated through the pancreas. The intensity of dye color in pancreatic juice was estimated photometrically or macroscopically. The dye color intensity decreased as follows; basic fuchsin, acridine red, new fuchsin, rhodamin B, phenol red and rhodamin 6G. Basic fuchsin consistently appeared in CCK-PZ stimulated juice. However, it was seen in only a scant amount or not at all in juice stimulated by purified Vitrum (Sweden) secretin. Similar findings were observed in cats and conscious pigs. The content of basic fuchsin in pancreatic juice was more related to changes in the enzyme concentration than to other components. The chloride content of the juice was related to the amylase or basic fuchsin secretion. However, the chloride content was inversely related to the secreted volume. Vagal stimulation or the administration of parasympathomimetics produced a juice rich in enzyme content, but the dye response to vagal stimulation was weak. Usually the volume of secreted pancreatic juice following stimulation by Boots (England) secretin is greater than stimulated by purified Vitrum preparation. Basic fuchsin was slightly reduced during its elimination from pancreas or when present in alkaline pancreatic juice. Adding acid and formaldehyde revived the color. The acridine red and other pyronine dyes caused the juice to fluorescence. This effect lasted over 24 hours.