ABSTRACT
O papiloma invertido é um tumor epitelial incomum que compromete a regiäo nasal e os seios paranasais. Este tumnor, embora originariamente benigno, tem alto grau de recorrência, podendo sofrer transformaçäo maligna. Ele pode destruir estruturas ósseas e se estender para as fossas cranianas anteriores e média, e tornar-se, desse modo, indistinguível radiologicamente de lesäo malígna. 8 pacientes com papiloma invertido histologicamente confirmados säo descritos e os seus achados radiológicos apresentados e comparados com os da literatura
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Head and Neck Neoplasms/analysis , Papilloma/analysis , Radiography/instrumentation , BrazilABSTRACT
The incidence of tumor and the time of expression, cellular localization and the molecular weight of tumor associated proteins of rat skin tumor induced by 7,12-dimethylbenz[a]anthracene (DMBA) with or without 12-O-tetradecanoyl-phorbol-13-acetate (TPA) were studied. The time of the development of skin tumors in 0.1% DMBA-TPA treated rats was significantly shorter than that in rats which were treated with DMBA alone. In the complete carcinogenesis case, papillomas developed more slowly and were less common and also squamous cell carcinomas appeared much later. From the analysis of the proteins of each experimental group by SDS-PAGE and two dimensional gel electrophoresis, at least three tumor associated proteins were identified (54kd, pl = 5.66; 27kd, pl = 5.85; 11kd, pl = 4.90). Also these proteins were found in rat dorsal skin from 14 days gestation to 21 days postpartum, and disappeared after 28 days. In conclusions, two stage skin carcinogenesis could be successfully demonstrated in Sprague-Dawley rats and abnormal proteins were produced in DMBA or DMBA-TPA induced skin tumor. The tumor associated proteins of skin tumor induced by DMBA or DMBA-TPA were appeared at the late initiation stage or early promotion stage, and they were localized in plasma membrane and were glycoproteins that are thought to be related to the epidermal differentiation process.
Subject(s)
Rats , 9,10-Dimethyl-1,2-benzanthracene , Animals , Antigens, Surface/analysis , Carcinoma, Squamous Cell/analysis , Cell Membrane/metabolism , Comparative Study , Glycoproteins/analysis , Membrane Proteins/analysis , Papilloma/analysis , Rats, Inbred Strains , Skin Neoplasms/analysis , Tetradecanoylphorbol AcetateABSTRACT
The incidence of tumor and the time of expression, cellular localization and the molecular weight of tumor associated proteins of rat skin tumor induced by 7,12-dimethylbenz[a]anthracene (DMBA) with or without 12-O-tetradecanoyl-phorbol-13-acetate (TPA) were studied. The time of the development of skin tumors in 0.1% DMBA-TPA treated rats was significantly shorter than that in rats which were treated with DMBA alone. In the complete carcinogenesis case, papillomas developed more slowly and were less common and also squamous cell carcinomas appeared much later. From the analysis of the proteins of each experimental group by SDS-PAGE and two dimensional gel electrophoresis, at least three tumor associated proteins were identified (54kd, pl = 5.66; 27kd, pl = 5.85; 11kd, pl = 4.90). Also these proteins were found in rat dorsal skin from 14 days gestation to 21 days postpartum, and disappeared after 28 days. In conclusions, two stage skin carcinogenesis could be successfully demonstrated in Sprague-Dawley rats and abnormal proteins were produced in DMBA or DMBA-TPA induced skin tumor. The tumor associated proteins of skin tumor induced by DMBA or DMBA-TPA were appeared at the late initiation stage or early promotion stage, and they were localized in plasma membrane and were glycoproteins that are thought to be related to the epidermal differentiation process.