ABSTRACT
INTRODUCTION: White piedra is a superficial mycosis caused by the genus Trichosporon and characterized by nodules on hair shaft. METHODS: The authors report a family referred to as pediculosis. Mycological culture on Mycosel® plus molecular identification was performed to precisely identify the etiology. RESULTS: A Trichosporon spp. infection was revealed. The molecular procedure identified the agent as Trichosporon inkin. CONCLUSIONS: White piedra and infection caused by T. inkin are rarely reported in Southern Brazil. The molecular tools are essentials on identifying the Trichosporon species.
INTRODUÇÃO: Piedra branca é micose superficial causada por fungos do gênero Trichosporon e caracterizado por nódulos firmemente aderidos à haste do pêlo. MÉTODOS: Os autores relatam casos familiares encaminhados como pediculose. Foram utilizados cultura em ágar Mycosel® e identificação molecular. RESULTADOS: Exame micológico revelou a infecção por Trichosporon spp. A identificação molecular demonstrou se tratar do Trichosporon inkin. CONCLUSÕES: Piedra branca e infecção pelo T. inkin são raramente relatados na região sudeste do Brasil. A identificação molecular é essencial para correta determinação de espécies no gênero Trichosporon.
Subject(s)
Adult , Child , Child, Preschool , Female , Humans , Male , Piedra/diagnosis , Scalp Dermatoses/diagnosis , Scalp Dermatoses/microbiology , Trichosporon/genetics , Piedra/microbiology , Trichosporon/isolation & purification , Trichosporon/classificationABSTRACT
The Trichosporon genus is constituted by many species, of which Trichosporon ovoides and Trichosporon inkin are the causative agents of white piedra. They can cause nodules in genital hair or on the scalp. At present, Brazilian laboratory routines generally do not include the identification of the species of Trichosporon genus, which, although morphologically and physiologically distinct, present many similarities, making the identification difficult. The aim of this study was to identify the aetiological agents at the species level of white piedra from clinical specimens. Therefore, both the macro and micro morphology were studied, and physiological tests were performed. Trichosporon spp. was isolated from 10 clinical samples; T. ovoides was predominant, as it was found in seven samples, while T. inkin was identified just in two samples. One isolate could not be identified at the species level. T. inkin was identified for the first time as a white piedra agent in the hair shaft on child under the age of 10.
Subject(s)
Adult , Child , Child, Preschool , Female , Humans , Male , Piedra/microbiology , Scalp Dermatoses/microbiology , Trichosporon/classification , Mycological Typing Techniques , Retrospective Studies , Trichosporon/chemistry , Trichosporon/cytologyABSTRACT
White piedra is a superficial mycosis caused by Trichosporon spp. that affects the hair shaft of any part of the body. It is presented an outbreak of scalp white piedra seen in 5.8 percent of the children frequenting a day care in Northeastern of São Paulo State, Brazil. Mycological exam and culture identified T. cutaneum in all five cases, and scanning electron microscopy of nodules around hair shaft infected by Trichosporon spp. is demonstrated comparing them with those of black piedra and with nits of Pediculous capitis.
Piedra branca caracteriza-se por ser micose superficial, causada por Trichosporon spp., que compromete a haste dos pelos de qualquer região do corpo. Um surto de piedra branca, afetando os cabelos do couro cabeludo, foi registrado em 5,8 por cento das crianças que freqüentavam uma creche na região nordeste do estado de São Paulo. Exame micológico direto e cultura identificaram T. cutaneum nas cinco crianças afetadas. Enfatiza-se a utilização da microscopia eletrônica de varredura, que mostrou nódulos circundando a haste dos cabelos infectada por Trichosporon spp., comparando-os com nódulos de Piedra nigra e com lêndeas de Pediculus capitis.
Subject(s)
Child, Preschool , Female , Humans , Child Day Care Centers , Piedra/epidemiology , Scalp Dermatoses/epidemiology , Trichosporon/isolation & purification , Brazil/epidemiology , Disease Outbreaks , Microscopy, Electron, Scanning , Piedra/diagnosis , Piedra/microbiology , Scalp Dermatoses/diagnosis , Scalp Dermatoses/microbiology , Trichosporon/ultrastructureABSTRACT
Os autores apresentam três casos de piedra branca comprometendo o couro cabeludo de crianças em idade pré-escolar. O Trichosporon beigelii foi caracterizado por exames micológicos. Revisa-se a literatura abordando aspectos clínicos, micológicos e terapêuticos
Subject(s)
Humans , Female , Child, Preschool , Child , Piedra/microbiology , Scalp/microbiology , Mercuric Chloride/therapeutic use , Piedra/drug therapy , Piedra/pathology , Trichosporon/isolation & purificationABSTRACT
No presente trabalho foi realizado estudo em microscopia eletrônica de varredura do fungo Piedraia hortae, através de material obtido de lesöes no cabelo de índios do Amazonas. O exame em microscopia eletrônica revelou ascosporos poliédricos, ovóides e arredondados, arranjados isoladamente ou na forma de pseudohifas, de permeio a material extracelular densamente compactado