ABSTRACT
Piperaceae is an important family from neotropical American in which many biologically active compounds were identified. A survey of structural diversity and bioactivity reveals that groups of species specialize in the production of amides, phenylpropanoids, lignans and neolignans, benzoic acids and chromenes, alkaloids, polyketides, and a plethora of compounds of mixed biosynthetic origin. With this in mind, several explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D, DICAMBA, BAP and KIN). The callus induction was depended of Piper species, explant type and plant growth regulator combinations. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieve results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of various Piper species.
Piperaceae es una familia importante de la América neotropical en la que muchos compuestos biológicamente activos han sido identificados. Un estudio general sobre la diversidad estructural y bioactividad reveló que grupos de especies se especializan en la producción de amidas, fenilpropanoides, lignanos y neolignanos, ácidos benzoicos y cromenes, alcaloides, policétidos y una diversidad de compuestos de origen biosintético mixto. Con este conocimiento, diversos explantes obtenidos de plántulas in vitro se cultivaron en presencia de diferentes combinaciones de reguladores de crecimiento (AIA, ANA, 2,4-D, DICAMBA, BAP y KIN). La inducción de callos dependió de la especie de Piper, tipo de explante y las combinaciones de los reguladores de crecimiento. Suspensiones celulares se establecieron a partir de callos y estudiadas en su crecimiento y acumulación de metabolitos. Los resultados alcanzados pueden utilizarse para caracterizar rutas de producción de metabolitos secundarios, en sistemas in vitro, en varias especies de Piper.
Subject(s)
Plant Growth Regulators , Piperaceae/growth & development , Piperaceae/metabolism , Cell Culture Techniques , Germination , Organogenesis , Piperaceae/embryology , Time FactorsABSTRACT
A Pothomorphe umbellata (Piperaceae), é uma espécie medicinal nativa do Brasil, utilizada na indústria de cosméticos e protetores de pele contra raios UVA e UVB. Com o intuito de gerar informações aplicadas à propagação da espécie, o presente trabalho relacionou a coloração e a massa de sementes de P. umbellata a seu comportamento germinativo. A coloração e a massa de sementes de P. umbellata foram características adequadas para avaliar a homogeneidade fisiológica, o vigor, o potencial e o comportamento germinativo. Assim, conclui-se que, embora de germinação lenta, as sementes de coloração preta e mais densa devem ser as escolhidas quando de coleta ou de processo seletivo.
Pothomorphe umbellata (Piperaceae) is a medicinal species native to Brazil, which has been used in the cosmetic industry and in products that protect the skin against UVA and UVB rays. To generate information applied to the species propagation, the present work related the coloration and the mass of P. umbellata seeds to their germinative behavior. The coloration and the mass of seeds of P. umbellata were characteristic appropriate to evaluate physiologic homogeneity, vigor, potential and germinative behavior. Thus, although of slow germination, seeds of black and denser coloration should be chosen during collection or selective process.