Influence of learning trials on memory retention of planaria: dugesia tigrina

The following research was conducted to determine: [1] the influence of learning trials on memory retention of planaria: dugesia tigrina, and [2] the extent of retention after a 2 day time period. Results indicated that learning trials do influence memory retention in planaria, and that the extent of retention is associated with the amount of prior training before the retesting

Labelling of the platyhelminth Dusegia tigrina with 99mTechnetium

The study of the labelling of planaria with 99mTc shows that the incorporation of radioactivity in this platyhelminth increases with an increase sin SnCl2 concentration from 0.13 to 1.3 micronM, reaching a plateau in the range of 1.3-130 micronM then decreasing with 1300 microng. At concentrations of 1.3 and 13 microngM SnCl2, a stronger binding of 99mTc was obtained. The biological viability of the labelled planaria was not altered when the described methodology was used. The advantage of this new labelling technique is that it is possible to obtain a platyhelminth preparation labelled with a radionuclide that is very cheap, is easily available and is a gamma emitter with a photon energy of 140 KeV