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Braz. j. infect. dis ; 12(6): 469-471, Dec. 2008. ilus
Article in English | LILACS | ID: lil-507443

ABSTRACT

The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety "Vitória de Verão" genetically modified.


Subject(s)
Hepatitis B Surface Antigens/genetics , Hepatitis B Vaccines/genetics , Lactuca/genetics , Plants, Genetically Modified/genetics , DNA, Recombinant , Lactuca/immunology , Plant Growth Regulators , Plants, Genetically Modified/immunology , Seedlings/genetics , Seedlings/immunology , Vaccines, Edible
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