Comparison of female reproductive organs morphology between fertile and infertile women with polycystic ovaries

To compare the morphology of pelvic reproductive organs between fertile and infertile women with polycystic ovaries. This cross sectional comparative study was performed at Rahat Hospital, Karsaz Karachi from January 2008 to December 2008. In which 100 women diagnosed with polycystic ovaries identified by ultrasound were inducted for the study; 50 were fertile and 50 were infertile [20-40 years]. Trans abdominal scan [TAS] was performed to rule out abnormalities other than PCO. Ovarian volume [OV] in cm[3], follicle count [FC] and size [FS] in mm, uterine area [UA] in cm[2], endometrial thickness [Endo] in mm were measured and noted by transvaginal scanning [TVS] ; Basal Metabolic Rate [BMI] was calculated and correlated with endometrial thickness. Unpaired t-test and Pearson correlation were used for analysis. In the total fertile and infertile women with PCO the following result was obtained OV-TAS 7.33 +/- 3.17 vs. 10.87 +/- 2.49 [0.001], OV-TVS 8.79 +/- 2.19 vs. 12.44 +/- 2.36 [0.001], FC 14.41 +/- 2.18 vs. 14.75 +/- 2.80 [0.245], FS 3.26 +/- 0.22 vs. 8.99 +/- 0.45 [0.001], BMI 25.6 +/- 4.7vs. 27.6 +/- 5.7 [0.04], UA 86.9 +/- 25.7 vs. 117.2 +/- 29.0 [0.001] and Endo 4.5 +/- 1.4 vs. 5.8 +/- 1.9 [0.052]. BMI and endometrial thickness showed a positive correlation with 'r' value of 0.06[0.05] in the infertile women and a negative correlation with 'r' value of - 0.40[0.05] in fertile women. The OV, FS, BMI, UA and Endo show a significant increase in the infertile women who are suffering from PCO. However the FC showed no significant difference between the two groups. The BMI and endometrial thickness has shown a positive correlation with infertility

Histochemical study of the extracellular matrix components in the follicular wall of induced polycystic ovaries

Numerous models have been developed to study polycystic ovarian syndrome in rats. In the present study, the syndrome was induced by exposure to constant light. The histological structure and differential distribution of extracellular matrix (ECM) fibers as well as the glycosaminoglycans (GAGs) content and composition of the ovarian follicular wall of rats with polycystic syndrome were evaluated. Histochemical differences were observed in the graunlosa and theca externa of follicular cysts when compared to normal preovulatory follicles. The colagen content of the theca externa of follicular cysts, quantified by the picrosirius method, was higher than in the controls. The neural carbohydrate and acidic GAC levels were lower in the granulosa and higher in the theca externa of cyst follicles than in control ovaries. Histomorphometrically, the follicular diameter was both a convenient and appropriate measurement for describing the cyst status; there were no differences in the thickness of each follicular layer. In conclusion, differences in the components of ECM were observed in the follicular wall of ovarian cysts compared eith normal preovulatory follicles. Howere, sinde these changes did not occur uniformly in all layers of the follicular wall, their role in cyst development remains to be established.

Inducción de un modelo murino de síndrome de ovarios poliquísticos: Valoración laparoscópica histológica y endocrina. Utilidad clínica / Induction of a murine model of the polycystic ovary syndrome: Laparoscopic, histologic and endocrine evaluation. Clinical usefulness

Objetivo: Evaluar el efecto del tratamiento endoscópico del síndrome de ovarios poliquísticos en un modelo murino inducido con estrógeno de depósito: Material y métodos: Grupo 1 (control, n = 15) ratas con inhibición de la actividad gonadotrópica y tres grupos con síndrome de ovarios poliquísticos: grupo 2 (n = 22) evaluadas exclusivamante por laparoscopia; grupo 3 (n = 11) fue efectuada una fulguración en cada ovario durante el procedimientos endoscópico; y grupo 4 (n = 10) con tres fulguraciones en cada ovario. Un mes después de la cirugía, los animales fueron sacrificados y se realizó estudio de los órganos intraabdominales mediante microscopio quirúrgico y evaluación histológica de las gónadas. Resultados: Todos los animales tuvieron síndrome de ovarios poliquísticos. Los del grupo 1 no desarrollaron adherencias; mientras que los del grupo 3 las presentaron en 36.3 por ciento de los casos y hubo atrofía gonadal en 18.1 por ciento. El 66.6 por ciento de las ratas del grupo 3 tuvieron adherencias intraabdominales. Hubo diferencia significativa en el grado de formación de adherencias cuando se compararon los grupos 3 y 4 con el grupo 2. Conclusiones: El uso de estrógeno de depósito es útil para la producción del síndrome de ovarios poliquísticos en animales de experimentación. El procedimiento puede emplearse para estudiar los efectos de la inducción quirúrgica de la ovulación; su utilización en otras condiciones también parece ser promisorio

Observaçöes morfológicas do ovário policístico de rata: I estudo histoquímico do colágeno / Morphological aspects of the ovary of the rat

Os autores descrevem os aspectos morfológicos do ovário de ratas em estro permanente, com ênfase para distribuiçäo dos tipos de colágeno, utilizando o método do Picrosírius-Polarizaçäo. Os dados obtidos säo indicativos de que o colágeno, particularmente os tipos Ie III, säo susceptíveis a hiperplasia na dependência de açäo hormonal