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Indian J Exp Biol ; 1997 Sep; 35(9): 1011-3
Article in English | IMSEAR | ID: sea-62744

ABSTRACT

Polypeptides synthesized from polysome-bound and unbound fractions of polyA mRNA were studied by in-vivo cells labelling and cDNA-mRNA hybrid-arrest translation. The polypeptides synthesized from unbound(free) fraction appeared to be significantly reduced in the absence of cycloheximide in L6 myoblast. The cDNA excess-mRNA hybrid-arrest translation exhibited rare polypeptide sequences in the unbound fractions.


Subject(s)
Animals , Chemical Fractionation , Muscles/metabolism , Peptides/analysis , Polyribosomes/chemistry , RNA, Messenger/chemistry , Rats , Ribonucleoproteins/metabolism
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