Trials for improving the efficiency of inactivated Newcastle disease virus vaccine by using propioni-bacterium and lipopolysaccharide [IM-104] as immunostimulant

The ability to improve the efficacy of an inactivated Newcastle disease vaccine by addition of immunomodulator as propionibacterium and lipopolysaccharide [TM-104] was studied. Chickens were divided into three groups, one received inactivated Newcastle vaccine containing the IM-104, second group received vaccine alone and third group of chickens was left without vaccination as control. Blood samples were collected 3 days post vaccination, and weekly till four weeks for estimation of the effect of [IM-104] on Tlymphocyte transformation [cellular immune response]. Sero-conversion for sera at weekly intervals was done to evaluate the immune response using HI test and ELISA. Challenge test was carried out on 3[rd] and 6[th] week post vaccination for evaluation of protective efficacy of these vaccines. The results obtained revealed that [TM-104] that contain 2 extraction of E. coli lipopolysaccharide and propioni-bacterium plays roles in stimulating both T-lymphocyte and macrophage to release different types of interleukin and consequently released immunoglobulins. Also, stimulate non-specific resistance to viral infection. The results obtained by challenge test run in parallel with that of serological test

Selection of microencapsulation method to improve antimicrobial agents production by some lactobacillus species and propionibacterium thoenii in dairy products

The aim of this research is to select the agreeable encapsulation method to improve antimicrobial production from Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus rhamnosus and Propionibacterium thoenii. The effect of different organic acid concentrations [1 and 2 w/v], different pH values [3, 4, 5, 6. 7 and 8], different temperature degrees [0, 7, 25, 37 and 45°C] and storage temperature on viability of encapsulated bacteria were investigated. Also, the efficiency of microencapsulated methods [alginate + NaCI, alginate + oil and K-carrageenan] on enhancement of antimicrobial production were studied. Microencapsulation with alginate + NaCI offered greater production in extreme conditions [low pH, low temperature and in the presence of organic acids]. In addition, this method was more effective against pathogenic bacteria by enhancement of antimicrobial production, thus it may be effectively used to increase the safety and the shelf- life of dairy products

Sensibilidad in vitro de microorganismos anaeróbicos / In vitro sensibility of anaerobic microbial

Los estudios in vitro de sensibilidad antibiótica frente a bacterias anaeróbicas son escasos en nuestro medio. El presente trabajo evaluó la actividad antimicrobiana de seis antibióticos frente a 301 cepas de microorganismos anaeróbicos. Destaca la sensibilidad del grupo Bacteroides fragilis grupo, frente a la clindamicina, en especial cuando se compara con la actividad de metronidazol. Los Bacteroides melaninogenicus presentan una resistencia de 33% al metranidazol, condición que no está descrita en la literatura extranjera. La penicilina y el cloramfenicol son los más activos frente a los microorganismos Gram positivos anaeróbicos. La actividad de metronidazol frente a este grupo de microorganismos es la más baja de todos los antibióticos estudiados. La clindamicina, el cloramfenicol y los nitroimidazólicos presentan mejor actividad que la penicilina frente a los Clostrium perfringens