Estudo do perfil de expressão de componentes da via de sinalização sonic hedgehog em displasia epitelial oral / Study of the expression profile of components of sonic hedgehog signaling pathway in oral epithelial dysplasia

A displasia epitelial oral (DEO) é um aspecto histológico descrito em lesões potencialmente malignas, cujos mecanismos relacionados a patogênese e potencial de transformação são pouco conhecidos. Sabendo-se que a via de sinalização Sonic Hedgehog(SHH)tem participação no desenvolvimento do carcinoma escamocelular de boca(CEB) e que as proteínas relacionadas a esta via de sinalização estão envolvidas nos mecanismos biológicos relacionados a iniciação e progressão de tumores humanos. O objetivo deste trabalho foi estudar a expressão das proteínas da via de sinalização SHH em DEO, a fim de contribuir para o conhecimento do perfil biológico desta lesão...

Oral epithelial dysplasia (OED) is a histological aspect described in premalignant lesions and themechanisms related to the pathogenesis and malignant progression are poorly understood. It is knownthat Sonic Hedgehog (SHH) signaling pathway participates in the development of oral squamous cellcarcinoma, and proteins related to this cascade are involved in biological mechanisms related to theinitiation and progression of human tumors. The aim of this study was to investigate SHH signalingmolecules in OED, in order to contribute to the knowledge of the biological profile of this lesion...

Intestinal ascending colon morphometrics in rats submitted to severe protein malnutrition / Morfometría de la pared intestinal del colon ascendente de ratas sometidas a desnutrición proteica severa

This paper aims to verify the effects of severe protein malnutrition over the intestinal ascending colon morphometrics in adult rats. 12 rats (90 days old) were divided into 2 groups: control (n = 5) and malnutritioned (n = 7). In the following 90 days, the rats of the control group received a 24 percent protein chow as the malnourished group received 4 percent protein chow. The animals were submitted to euthanasia according to the anesthetic protocol. Colon segments were collected and submitted to routine histological processing. The cuts were stained with HE and histochemical techniques for mucines. The morphometric analyses showed the sustenance of the whole wall and muscle tunic thickness, as well as the reduction of the thickness of the mucosa tunic, the amount of goblet cells, the depth of the crypt and the height of the enterocytes as well as their nucleus on malnutritioned animals. The data suggest that protein malnutrition causes alterations on adult rat ascending colon intestinal morphometrics, especially in tissues which present a high level of cell turnover such as the mucosa tunic and consequently their structures such as the enterocytes, goblet cells, and crypts

El objetivo de este trabajo fue verificar los efectos de la desnutrición proteica severa sobre la morfometría de la pared intestinal del colon ascendente de ratas adultas. Fueron utilizadas 12 ratas (90 días de edad), divididas en dos grupos: control (n=5) y desnutrido (n=7). En los 90 días siguientes, las ratas del grupo control recibieron ración con 24 por ciento de contenido proteico y los del grupo desnutrido con 4 por ciento. Los animales fueron eutanasiados de acuerdo al protocolo anestésico. Segmentos del colon fueron retirados y sometidos a procesamiento histológico de rutina. Los cortes fueron teñidos con HE y técnicas histoquímicas para mucinas. El análisis morfométrico mostró la mantención de la pared total y del grosor de la túnica muscular, y reducción en el espesor de la túnica mucosa, en el número de células caliciformes, en la profundidad de las criptas y en la altura de los enterocitos y de sus núcleos, en los animales desnutridos. Los datos obtenidos sugieren que la desnutrición proteica provoca alteraciones en la morfometría intestinal del colon ascendente de ratas adultas, principalmente en tejidos de alto índice de renovación celular como la mucosa y, consecuentemente, de sus estructuras como los enterocitos, células caliciformes y criptas

Structural changes in the jejunal mucosa of mice infected with Schistosoma mansoni, fed low or high protein diets

The effects of high and low-protein diets on the structure of the jejunal mucosa were studied in Schistosoma mansoni infected mice (morphology and histomorphometry). Weaning male albino mice were infected with 80 cercariae, fed with high (20 percent) or low-protein (5 percent) diets and compared to uninfected controls under the same conditions. Mice were sacrificed 12 weeks after infection. Animals submitted to a low-protein diet showed lower weight curves, mainly when infected. In the jejunal mucosa, finger-like villi were the predominant pattern among uninfected high-protein fed animals, while the infected ones showed leaf-shaped and flattened villi in most cases. Undernourished infected mice had 65.7 percent leaf-shaped villi. A significant increase in the number of goblet cells was seen in infected mice. A decrease in the number of absorptive cells was detected in undernourished mice, particularly in infected ones

Effects of maternal proteic undernutrition on the neurons of the myenteric plexus of the duodenum of rats

The purpose of this study was to verify the effects of proteic undernutrition on the neurons of the myenteric plexus from the duodenum of Wistar rats. Twenty-four animals at the age of 60 days were divided iii four groups, which were named according to the period their mothers received hypoproteic ration (8 per cent). Some segments of duodenum were subjected to histological treatment and stained with hematoxilin-eosin and some were used for whole mount preparations stained with Giemsa. We observed small, medium-sized and large neurons grouped in ganglia of various shapes. It was concluded that the maternal proteic undernutrition does not affect the organization of the myenteric plexus and that animals submitted to undernutrition does not affect the organization of the myenteric plexus and that animalssubmitted to undernutrition during gestation and lactation, wheil nornlally fed, sliow iielirons with strongly basophilic cytoplasin aiid larger cellul@ir bodies than tliose from control animals.

Histobiochemical changes in lung of protein deficient rats following repeated exposures of MIC vapour.

Adult male albino rats, maintained on normal or protein deficient diets from weanling, were exposed to repeated doses of MIC vapour (0.32 mg/L for 8 min for 5 consecutive days) under static conditions. Histopathology and the activities of alkaline and acid phosphatases and GSH content of lung were studied upto day 14 after exposure. Mild but repeated exposures of MIC vapour caused severe pulmonary lesions like denudation of bronchiolar epithelial lining tissue, cellular infiltration, edema, emphysema followed by hyperplasia, hypertrophy, fibrosis and intraluminal fibroplasia. The activities of alkaline and acid phosphatases were increased at earlier intervals while GSH content decreased significantly and remained low throughout the experimental duration. Protein deficiency was found to aggravate the toxic potentials of MIC in present condition.

Glucose modulation of gap junctions in the islets of Langerhans of protein malnourished rats.

It is believed that protein deficiency causes decreased insulin release in response to a glucose stimulus. We have recently shown that in prolonged protein deficiency, decreased insulin response to glucose is directly proportional to a decrease in the islet volume, suggesting no apparent defect in the insulin secretory mechanism in protein deficiency. It is documented that glucose-stimulated insulin release is closely related to an increase in the gap junctions of stimulated islet beta cells. In the present study, we show that the gap junctions of islets obtained from rats fed on a 4% protein diet were increased both in number and size following glucose treatment. This provided further proof that the mechanism to respond to glucose is not compromised in the endocrine tissue of the severely protein malnourished rats.