ABSTRACT
A simple, accurate and rapid high performance thin layer chromatography [HPTLC]-densitometric method was developed for separation and determination of cetirizine [CET] as a long acting antihistamine and montelukast [MON] as an antileukotriene in pharmaceutical dosage forms. The compounds were separated on silica gel 60 F[254] HPTLC plates using a mixture of ethyl acetate: methanol: ammonia solution [25%] [14: 3: 2 v/v/v] as mobile phase. The plates were developed vertically up to a distance of 80 mm. Compact spots of both cetirizine [R[f] = 0.30 +/- 0.01] and montelukast [R[f] = 0.52 +/- 0.02] were obtained. UV detection was performed at 230 nm. Quantitative analysis was performed by absorbance densitometry using peak area. The method was validated in terms of linearity, precision, accuracy, limit of detection [LOD], and limit of quantification [LOQ]. The calibration curves were linear in the range of 40-2000 ng spot[-1] for cetirizine and 120-1000 ng spot[-1] for montelukast. For MON, recovery varied in range of 99.20-100.88% with RSD ranging from 1.02 to 1.90% and for CET, recovery varied in range of 98.13-100.05% with RSD ranging from 1.57 to 1.85%. The LODs were found to be 3.94 and 2.08 ng spot[-1] for CET and MON, respectively. It was observed that the proposed HPTLC method could be used for efficient analysis and monitoring of the CET and MON in combined tablet dosage forms, more convenient with better precision and accuracy than HPLC method
Subject(s)
Cetirizine/chemistry , Acetates/chemistry , Quinolines/chemistry , Chromatography, High Pressure Liquid/methods , Quinolines/isolation & purification , Acetates/isolation & purification , Cetirizine/isolation & purification , Dosage Forms , Reproducibility of Results , Densitometry , Pharmaceutical PreparationsABSTRACT
Following our phytochemical studies of Costa Rican plants, in this work we report the isolation and identification of eight compounds from aerial parts of Zanthoxylum setulosum (Rutaceae). They were identified as the alkaloid skimmianine, the lignans savinin, kusunokinin, sesamin, syringaresinol and the isopentenyl ether of pluviatol, the amide aurantiamide acetate, and the triterpen lupeol. This is the first report of isolation of skimmianine from the leaves of Z. setulosum and its presence confirm that quinoline and benzophenanthridine alkaloids, can be considered as chemotaxonomic markers of this genus. All the isolated compounds were characterized by spectroscopic methods (including 1H-NMR, 13C-NMR, , HMQC, HMBC and NOESY) and comparison with the literature data.
Continuando con el estudio fitoquímico de plantas de Costa Rica, en este trabajo informamos el aislamiento e identificación de ocho compuestos de las partes aéreas de Zanthoxylum setulosum (Rutaceae). Los compuestos fueron identificados como el alcaloide skimmianina, los lignanos savinina, kusunokinina, sesamina, siringaresinol y el éter isopentílico del pluviatol, la amida conocida como acetato de aurantiamida, y el triterpeno lupeol. Este es el primer informe del aislamiento de skimmianina en las hojas de Z. setulosum, lo cual confirma que alcaloides quinolínicos y benzofenantridinicos pueden ser considerados marcadores quimiotaxonómicos en éste género. La estructura de los compuestos aislados fue caracterizada por métodos espectroscópicos (incluyendo 1HNMR, 13C-NMR, HMQC, HMBC y NOESY) y comparación con datos de la literatura.