ABSTRACT
The present study involves preparation and characterization of a combination tablet of ranitidine in immediate release form and domperidone in sustained release form, using ion exchange resins. Ranitidine lowers acid secretion, while domperidone release over a prolonged period improves gastric motility thus justifying this combination in gastro esophageal reflux diseases (GERD) and ensuring patient compliance. Drug loading was carried out by batch method & resinates were characterized using FTIR, XRPD. Resinates were formulated as a combination tablet and evaluated for tablet properties & in vitro drug release. Resinates provided sustained release of domperidone and immediate release of ranitidine. IR and X-ray studies indicate complexation of drug and resin along with monomolecular distribution of drugs in amorphous form in the resin matrix. The tablets of resinate combination showed good tablet properties. In-vitro drug release gave desired release profiles and ex-vivo drug absorption studies carried out by placing everted rat intestine in dissolution medium indicated statistically significant similarity in absorption from test and marketed formulation. The novelty of this study is that the retardation in release of domperidone from resinates is achieved by presence of weak resin in the formulation.
O presente estudo envolve a preparação e a caracterização de associação do comprimido de ranitidina de liberação imediata e domperidona de liberação prolongada, utilizando resinas de troca iônica. A ranitidina diminui a secreção ácida, enquanto a liberação prolongada de domperidona melhora a motilidade gástica, justificando, dessa forma, a associação em doenças de refluxo gastroesofágico (DRGE) e garantindo a adesão do paciente. A carga de fármaco foi efetuada pelo método em batelada e os resinatos, caracterizados utilizando-se FTIR e XRPD. Os resinatos foram formulados como comprimido da associação e avaliados com relação às propriedades dos comprimidos e liberação do fármaco in vitro. Os resinatos proporcionaram a liberação prolongada da domperidona e a liberação imediata da ranitidina. IV e estudos de difração de raios X indicaram a complexação do fármaco e da resina junto com a distribuição monomolecular dos fármacos, em estado amorfo, na matriz da resina. Os comprimidos da associação do resinato apresentaram boas propriedades. Obtiveram-se os perfis de liberação in vitro e os estudos de absorção dos fármacos ex vivo realizados com intestino de rato em meio de dissolução indicaram semelhança significativa na absorção entre as formulações teste e comercializada. A inovação do trabalho é que o retardamento da liberação da domperidona dos resinatos é atingido pela presença de resina fraca na formulação.
Subject(s)
Rats , Gastroesophageal Reflux , In Vitro Techniques , Ion Exchange Resins , Therapeutics , Domperidone/chemistry , Ranitidine/chemistryABSTRACT
A simple, specific and accurate reverse phase liquid chromatographic method has been developed for the simultaneous determination of naproxen and ranitidine HCl. Both the drugs are official with British Pharmacopoeia 2007, but do not involve simultaneous determination of naproxen and ranitidine HCl. The separation was carried out using 4.6x250 mm Symmetry Shield TM RP 18 with a particle diameter of 5 micro m and mobile phase containing 0.1M orthophosphoric acid: methanol [35:65, pH 3.1] in isocratic mode. The flow rate was 1.00 ml/min and effluent was monitored at 240 nm. The retention times [average] of ranitidine HCl and naproxen were 2.36 min and 12.39 min, respectively. The linearity for naproxen and ranitidine HCl was in the range of 5-35 micro g/ ml and 1.5-12 microg/ ml, respectively. The potencies of naproxen and ranitidine HCl were found 99.40% and 99.48%, respectively. The proposed method was validated and successfully applied to the estimation of naproxen and ranitidine HCl in newly formulated combined tablet and in plasma
Subject(s)
Ranitidine/chemistry , Chromatography, High Pressure Liquid , Phosphoric Acids , MethanolABSTRACT
In one case, viscera of a lady was received, who expired following injection given by doctor in urban area. After conducting elaborate analysis data were found similar to Ranitidine. Hence, it was thought worthwhile to concentrate on this drug .The present paper describes the extraction of Ranitidine from visceral material and its identification by thin layer chromatography using suitable solvent system and potassium iodo bismuthate as locating reagent.
Subject(s)
Chromatography, Thin Layer/methods , Humans , Ranitidine/analysis , Ranitidine/chemistry , Viscera/chemistrySubject(s)
Humans , Cholinergic Agents , Anti-Ulcer Agents , Antidiarrheals , Cathartics/classification , Gastric Acid , Aminosalicylic Acids/pharmacology , Cimetidine/chemistry , Colitis, Ulcerative/drug therapy , Crohn Disease/drug therapy , Dopamine Antagonists/pharmacology , Gastrointestinal Motility/drug effects , Immunosuppressive Agents/pharmacology , Inflammatory Bowel Diseases/drug therapy , Nizatidine/adverse effects , Ranitidine/chemistryABSTRACT
The conformational characteristics of two H2-receptor antagonists, cimetidine and ranitidine, have been investigated by quantum mechanical PCILO method and the results indicate a folded conformation for cimetidine stabilized by intramolecular hydrogen bonding and an extended backbone conformation for ranitidine. NMR investigations carried out on these two drugs in solution, however, indicate a predominance of an extended conformation for both the molecules. The significance of this result has been discussed in terms of the activity of these two drugs. Besides these studies, NMR experiments have also been carried out on the drugs incorporated into the lipid bilayers to investigate the drug-lipid interaction. The results from this study suggest that the hydrophobic portion of the drugs is buried in the hydrophobic hydrocarbon chains of the lipid bilayer, while the terminal hydrophilic end of the drug lies at the lipid-water interface.