ABSTRACT
Percoll-purified Leydig cells were studied in vitro in order to evaluate the effect of adrenaline on testosterone (T) secretion. Adrenaline enhanced the basal and potentiated the hCG-induced T release. A similar effect was also obtained with the ß2-agonist, salbutamol. Although phenyleprine, an alfa1-agonist, did not alter the basal T secretion, it enhanced hCG-mediated T secretion. Para-aminoclonidine, an alfa2-agonist, decreased the basal T release without altering the HCG-stimulated T release. These data demonstrate that either inhibitory or stimulatory effects on T release can be obtained and that they depend on the adrenoreceptor subtype involved
Subject(s)
Rats , Animals , Male , Chorionic Gonadotropin/pharmacology , Epinephrine/pharmacology , In Vitro Techniques , Leydig Cells/biosynthesis , Receptors, Adrenergic/pharmacology , Testosterone/metabolism , Rats, Inbred Strains , Testosterone/metabolismABSTRACT
Säo estudadas in vitro as açöes de diferentes concentraçöes de adrenalina, noradrenalina ou teofilina sobre a lipólise no tecido adiposo de pombos. Relata-se, ainda, a açäo do propranolol e da fentolamina sobre aquelas respostas lipolíticas. Os resultados mostram que doses elevadas de adrenalina ou noradrenalina ocaciosam declínio da lipólise no tecido adiposo de pombos, e que säo necessárias altas concentraçöes de teofilina para que a resposta lipolítica a esta droga se manifeste. Verificou-se que níveis elevados daqueles bloqueadores adrenérgicos, inibem a resposta lipolítica do tecido adiposo daquela ave, possivelmente também em algum passo posterior à formaçäo do AMP cíclico