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1.
Rev. bras. parasitol. vet ; 28(2): 333-337, Apr.-June 2019. tab, graf
Article in English | LILACS | ID: biblio-1042506

ABSTRACT

Abstract The objectives of this study were to describe occurrences of Rhabditis spp. causing parasitic otitis in dairy cattle of Gir breed in the state of Espírito Santo, southeastern Brazil, and to evaluate the biological control of this nematode using the nematophagous fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34). After nematode detection and collection, three groups were formed: two groups that were treated, respectively, with the fungal isolates; and a control group, without fungus. The treatments were as follows: (a) Petri dishes containing the culture medium 2% water agar (WA) + 250 nematodes + AC001; (b) Petri dishes containing 2% WA + 250 nematodes + NF34; and (c) Petri dishes containing only 2% WA + 250 nematodes. After seven days at 27 °C the treatments with fungi were able to capture and destroy the nematodes, with percentages of 82.0% (AC001) and 39.0% (NF34) in relation to the control group. The results demonstrate the occurrence of Rhabditis spp. after animals physical examination and that there was efficacy of the in vitro predatory activity of both fungal isolates. Thus, these results are important because they can assist in future in vivo control of this nematode in cattle.


Resumo Os objetivos neste estudo foram descrever ocorrências do nematódeo Rhabditis spp., causando otite parasitária em bovinos leiteiros da raça Gir no estado do Espírito Santo, sudeste do Brasil, e avaliar o controle biológico desse nematódeo utilizando os fungos nematófagos Duddingtonia flagrans (AC001) e Monacrosporium thaumasium (NF34). Após a detecção e coleta dos nematódeos, três grupos foram formados: dois grupos que foram tratados com os isolados fúngicos, respectivamente; e um grupo controle, sem fungos. Os tratamentos foram os seguintes: (a) placas de Petri contendo o meio de cultura 2% ágar de água (WA) + 250 nematoides + AC001; (b) placas de Petri contendo 2% de WA + 250 nematoides + NF34; e (c) placas de contendo apenas 2% de nematódeos WA + 250. Após sete dias a 27 °C os tratamentos com fungos foram capazes de capturar e destruir os nematódeos, com porcentagens de 82,0% (AC001) e 39,0% (NF34) em relação ao grupo controle. Os resultados demonstram a ocorrência de Rhabditis spp., no Estado do Espírito Santo e a eficácia da atividade predatória in vitro dos isolados fúngicos utilizados. Assim, esses resultados são importantes, pois podem auxiliar no controle alternativo in vivo de Rhabditis spp. em bovinos com otite parasitária.


Subject(s)
Animals , Cattle , Otitis/veterinary , Cattle Diseases/parasitology , Pest Control, Biological/methods , Rhabditoidea/microbiology , Rhabditida Infections/veterinary , Otitis/parasitology , Otitis/therapy , Ascomycota/physiology , Rhabditida Infections/therapy , Duddingtonia/physiology
2.
Braz. j. biol ; 77(3): 569-579, July-Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-888766

ABSTRACT

Abstract Entomopathogenic nematodes (EPN) belonging to the Heterorhabditidae family are lethal parasites of soil-dwelling insects. Two species were reported in Argentina: Heterorhabditis argentinensis and Heterorhabditis bacteriophora characterized mainly by morphometric features. In this work a comparative and phylogenetic study between five Heterorhabditis populations from Argentina was conducted to analyze the variability between strains and to evaluate the taxonomic position of Heterorhabditis argentinensis. The PCA analyses of morphometric characters separated the larger juvenile, female and male H. argentinensis from H. bacteriophora populations. The juvenile (IJs) stage provided the clearest separation of Heterorhabditis populations presenting the least variability between strains. The variable L and MBW were highly related to H. argentinensis IJs. Three groups were separated by this stage considering PC1 and PC2: one formed by H. bacteriophora OLI, RIV and RN strains, (isolates from Córdoba and Río Negro province), one for H. bacteriophora VELI strain (Buenos Aires province) and one for H. argentinensis (Santa Fe province). Heterorhabditis bacteriophora VELI and H. argentinensis isolated from regions with more rainfalls and humidity presented larger values for morphometric features. Molecular analyses showed the Argentinian populations (H. bacteriophora VELI strain and H. argentinensis), forming a same clade, with six other H. bacteriophora populations (not from Argentina) with a genetic similarity between them of 99%. Heterorhabditis argentinensis presented one unique nucleotide that was not present in any of the other species of the clade. Considering the results of this study H. argentinensis would be conspecific to H. bacteriophora, constituting a strain with a great morphometric variation where the host and climatic conditions could have influenced on the measurements.


Resumo Nematóides entomopatogênicos (EPN) pertencentes à família Heterorhabditidae são parasitas letais de insetos que vivem no solo. Duas espécies foram relatados na Argentina: Heterorhabditis argentinensis e Heterorhabditis bacteriophora, caracterizada principalmente por características morfométricas. Neste trabalho um estudo comparativo e filogenética entre cinco populações do Heterorhabditis da Argentina foi conduzido para analisar a variabilidade entre as linhagens e avaliar a posição taxonômica das Heterorhabditis argentinensis. Características morfométricas de Heterorhabditis bacteriophora VELI e H. argentinensis isoladas de regiões com mais chuvas e umidade apresentaram dimensões maiores. Analisa o PCA de personagens morfométricas separou a maior juvenil, feminino e masculino H. argentinensis de H. bacteriophora populações. A fase juvenil (JIs) fornece a mais clara separação de populações Heterorhabditis apresentando a menor variação entre as cepas. A L variável e MBW foram altamente relacionada com H. argentinensis JIs. Três grupos foram separados por esta fase considerando PC1 e PC2: um formado por H. bacteriophora OLI, RIV e estirpes RN, (isolados de Córdoba e província de Rio Negro), um para a estirpe H. bacteriophora VELI (província de Buenos Aires) e um para H. argentinensis (província de Santa Fe). Heterorhabditis bacteriophora VELI e H. argentinensis isolado a partir de regiões com mais chuvas e umidade apresentaram maiores valores para as características morfométricas. A análise molecular mostrou as populações da Argentina (estirpe H. bacteriophora VELI e H. argentinensis), formando um mesmo subtipo, com seis outras populações H. bacteriophora (não da Argentina), com uma similaridade genética entre eles de 99%. Heterorhabditis argentinensis apresentado um único nucleótido que não estava presente em nenhum dos outros espécies do clado. Considerando os resultados deste estudo H. argentinensis seria conspecific a H. bacteriophora, constituindo uma estirpe com uma grande variação morfométrica onde o anfitrião e as condições climáticas podem ter influenciado nas medições.


Subject(s)
Animals , Male , Female , Rhabditoidea/anatomy & histology , Rhabditoidea/classification , Phylogeny , Argentina , Rhabditoidea/physiology , Rhabditoidea/genetics , DNA, Helminth/genetics , Biological Control Agents , Insecta/growth & development , Insecta/parasitology , Larva/growth & development , Larva/parasitology
3.
Pesqui. vet. bras ; 36(7): 605-610, jul. 2016. ilus
Article in Portuguese | LILACS, VETINDEX | ID: lil-794765

ABSTRACT

O presente estudo teve como objetivo descrever a ocorrência de otite parasitária causada por Rhabditis blumi em bovinos leiteiros de raça Gir de uma fazenda da região Norte do Brasil. Foram coletadas amostras de 42 bovinos por swab e lavado dos condutos auditivos externos (CAE). Ao exame clínico, 9,5% (4/42) dos bovinos apresentavam sintomatologia nervosa, como leve a moderada rotação da cabeça, apatia, flacidez dos lábios e ptose palpebral unilateral, alopecia das regiões da cabeça e cupim, causados pelo desconforto e prurido da região auricular, alteração na mastigação e acúmulo de alimento na cavidade oral. Adicionalmente, 71,4% (30/42) dos abovinos mostraram a presença do parasita no cerúmen dos condutos auditivos. À análise microscópica do material do saco conjuntival foi observado presença do parasita em 90% (9/10) dos bovinos avaliados. Os 30 bovinos positivos para Rhabditis spp. foram distribuídos aleatoriamente em três grupos de 10 animais: (G1) Bovinos controle, (G2) Bovinos tratados com ivermectina 1% pour on e (G3) Bovinos submetidos a lavado dos condutos auditivos externos (CAE). Cada tratamento foi repetido três vezes com intervalo de sete dias. No G1 os 10 bovinos mantiveram-se infectados durante todo o estudo. No G2 20% dos bovinos foram negativos após os dois primeiros tratamentos, porém, mostraram-se positivos na terceira avaliação. No G3 todos os bovinos mantiveram-se positivos, sendo observada apenas diminuição da carga parasitária. A identificação por análise molecular por meio de fragmentos amplificados da expansão D2/D3 do 28S rDNA confirmou a presença apenas da espécie Rhabditis blumi nos animais. Baseado nas observações clínicas, morfológicas e moleculares pode-se relatar o primeiro caso de R. blumi em bovinos da raça Gir no Estado do Pará, através da compra de animais oriundos de áreas onde a otite parasitária tem sido diagnosticada, principalmente de Minas Gerais, para formar animais mestiços (Gir x Holandês). Desta forma ressalta-se a importância do exame clínico prévio dos animais a serem transferidos para outras propriedades ou regiões. Este relato também parece ser o primeiro sobre a presença de R. blumi no saco conjuntival de bovinos. O tratamento com ivermectina no G2 não surtiu melhora clínica dos bovinos.(AU)


This study aimed to describe the occurrence of parasitic otitis caused by Rhabditis blumi in dairy cattle of the Gir race from a farm in northern Brazil. Forty-two samples were collected from cattle by swab washed from the external auditory canal (EAC). On clinical examination, in 71.4% (30/42) of the cattle the parasite was found in the cerumen of the ear canal, along with alopecia of head and hump caused by discomfort and itching of the auricular region. At microscopic analysis of material from the conjunctival sac the parasite was found in 90% (9/10) of the evaluated cattle. In addition, 9.5% (4/42) of the cattle showed nervous symptoms, such as mild to moderate rotation of the head, apathy, flaccid lips and unilateral ptosis, change in chewing and food accumulation in the oral cavity. Thirty cattle positive for Rhabditis spp. were randomly divided into three groups of 10 animals each: (G1) Cattle Control, (G2) Cattle treated with ivermectin 1% pour-on, and (G3) Cattle undergoing wash of the external auditory canal (EAC). Each treatment was repeated three times with intervals of seven days. In G1, 10 cattle remained infected throughout the study. In G2, 20% of the cattle were negative after the first two treatments, however were positive at the third evaluation. In G3, all cattle remained positive, but with decrease in parasite load. Identification by molecular analysis of amplified fragments through the expansion D2/D3 28S rDNA confirmed the presence of only Rhabditis blumi. Based on clinical, morphologic and molecular examination, it appears to be the first report of the occurrence of R. blumi infection in Gir cattle in the State of Pará, due to the purchase of cattle from areas where parasitic otitis has been diagnosed, as from Minas Gerais, to produce crossbred animals (Gir x Holstein). This emphasizes the importance of prior clinical examination by the veterinary service in order to transfer only healthy animals to other properties or regions. This appears also to be the first report on R. blumi infection of the conjunctival sac in cattle. Treatment with ivermectin in G2 did not produce clinical improvement.(AU)


Subject(s)
Animals , Cattle , Ivermectin/therapeutic use , Otitis/diagnosis , Otitis/parasitology , Otitis/therapy , Rhabditoidea/parasitology , Genome , Parasitic Diseases/diagnosis
5.
Rev. patol. trop ; 42(2): 201-207, abr.-jun. 2013. tab
Article in Portuguese | LILACS | ID: lil-696199

ABSTRACT

O consumo de vegetais in natura é recomendado como alimentação saudável em razão de seuconsiderável percentual de vitaminas, sais minerais e fibras alimentares. Frequentemente as verduras são adubadas com dejetos ou irrigadas com águas contaminadas com matéria fecal. Em razão da ocorrência de enteroparasitos em consumidores de hortaliças, avaliou-se, por meio deste trabalho, a presença de parasitos em alfaces hidropônicas e convencionais comercializadas na cidade de Anápolis-GO. Utilizado o método de sedimentação espontânea, foram encontradas larvas de nematoides do gênero Rhabditis. Neste gênero, há espécies de vida livre que, eventualmente, podem parasitar o homem e animais domésticos. Diante dessas evidências, recomenda-se a higienização adequada destes alimentos tanto pelos consumidores domésticos como pelos manipuladores de hortaliças a serem servidas em restaurantes e lanchonetes. Trata-se do primeiro relato de Rhabditis em hortaliças no Brasil.


Subject(s)
Lactuca , Rhabditoidea/parasitology , Vegetables , Brazil
6.
Pesqui. vet. bras ; 33(3): 319-325, Mar. 2013. ilus, graf, tab
Article in English | LILACS | ID: lil-674378

ABSTRACT

We evaluated the dynamics of ear infestations caused by Rhabditis spp. and Raillietia spp., which were correlated with animal age, intensity of clinical signs and climate factors. Sixty-four Gir cattle were distributed into three groups: GA - 23 calves with 4 to 6 months of age; GB - 18 calves with 7 to 12 months of age; and GC - 23 heifers with 13 to 33 months of age. Five samplings, defined as S1, S2, S3, S4 and S5 were performed every three months from August 2008 to August 2009. The ear secretion was collected using the auricular washing method for the right ear and a swab for the left ear. A clinical assessment of the animals was performed, and they were classified according to the presence and severity of otitis. The highest relative frequency of rhabditosis was 52.2% in GC at the last sampling. In the first sampling, 42.2% of the animals were infested by Raillietia spp. The older cattle were more susceptible to infestations by both parasites. No correlation of Rhabditis spp. and Raillietia spp. parasitism with climate factors was found. The results showed that both parasites could infest Gir cattle, and in most cases, there was no co-infestation. Only older animals parasitized by the nematode showed clinical signs of the disease.


Avaliou-se a dinâmica da infestação auricular causada por Rhabditis spp. e por Raillietia spp., relacionando-a com a idade dos animais, com a intensidade dos sinais clínicos e com os fatores climáticos. Utilizaram-se 64 bovinos Gir, distribuídos em três grupos: GA - 23 bezerros de quatro a seis meses de idade, GB - 18 bezerros de sete a 12 meses e GC - 23 novilhas de 13 a 33 meses. Foram feitas cinco coletas a cada três meses. A secreção auricular foi coletada utilizando o método de lavagem auricular na orelha direita e um swab na orelha esquerda. Fez-se avaliação clínica dos animais classificando-os de acordo com a presença e gravidade da otite. A maior frequência relativa da rhabditiose encontrada foi de 52,2%, no GC na ultima coleta. Na primeira coleta, 42,2% dos animais estavam infestados pela Raillietia spp. Os bovinos mais velhos foram mais suscetíveis às infestações de ambos os parasitos. Não foi encontrada correlação entre o parasitismo por Rhabditis spp. e por Raillietia spp. e os fatores climáticos. Concluiu-se que ambos os parasitas podem infestar bovinos da raça Gir e na maioria dos casos, não ocorreram infestações simultâneas. Apenas os animais mais velhos, parasitados pelo nematóide, apresentaram sinais clínicos da doença.


Subject(s)
Animals , Cattle , Cestoda/isolation & purification , Cestode Infections/veterinary , Rhabditida Infections/veterinary , Rhabditoidea/isolation & purification , Labyrinthitis/veterinary , Otitis Media/veterinary
7.
Braz. j. biol ; 72(4): 929-933, Nov. 2012.
Article in English | LILACS | ID: lil-660391

ABSTRACT

The parameters of infection by lung parasites from two sympatric lizards, Mabuya arajara and Anolis brasiliensis, from the Atlantic Rainforest of the lower slope of Chapada do Araripe in Northeastern Brazil were analyzed between September, 2009 and July, 2010. A total of 202 lizards were collected. 125 specimens were from Mabuya arajara and 77 from Anolis brasiliensis. M. arajara was infected by the pentastomid Raillietiella mottae while A. brasiliensis was infected by the nematode Rhabdias sp., with an overall prevalence of 1.6% and 28.6%, respectively. The mean intensity of infection by Rhabdias sp. was 3.63 ± 2.58 (range 1-15). The body size and sex of lizards did not influence the intensity of infection by Rhabdias sp. The overall prevalence was also not different between males and females hosts in A. brasiliensis. Both Anolis brasiliensis and Mabuya arajara represent a new host to Rhabdias sp. and Raillietiella mottae, respectively.


Os parâmetros de infecção por parasitas pulmonares em dois lagartos simpátricos, Mabuya arajara e Anolis brasiliensis, da floresta úmida da encosta da Chapada do Araripe, Nordeste do Brasil, foram analisados entre setembro de 2009 e julho de 2010. Um total de 202 lagartos foi coletado, sendo 125 espécimes de Mabuya arajara e 77 de Anolis brasiliensis. M. arajara estava infectado pelo pentastomídeo Raillietiella mottae, enquanto A. brasiliensis apresentava infecção pelo nematódeo Rhabdias sp., com prevalência total de 1,6% e 28,6%, respectivamente. A intensidade média de infecção por Rhabdias sp. foi 3,63 ± 2,58 (amplitude 1-15). O tamanho e o gênero dos lagartos não influenciaram a intensidade de infecção por Rhabdias sp. A prevalência também não apresentou diferença entre machos e fêmeas em A.brasiliensis. Ambos, Anolis brasiliensis e Mabuya arajara, representam novos hospedeiros para Rhabdias sp. e Raillietiella mottae, respectivamente.


Subject(s)
Animals , Female , Male , Lizards/parasitology , Lung Diseases, Parasitic/veterinary , Pentastomida , Parasitic Diseases, Animal/epidemiology , Rhabditida Infections/veterinary , Rhabditoidea/isolation & purification , Brazil/epidemiology , Lizards/classification , Lung Diseases, Parasitic/epidemiology , Lung Diseases, Parasitic/parasitology , Prevalence , Parasitic Diseases, Animal/parasitology , Rhabditida Infections/epidemiology
8.
Pesqui. vet. bras ; 32(9): 855-858, set. 2012.
Article in English | LILACS | ID: lil-654364

ABSTRACT

This study aimed to know the perception of Gir cattle breeders and farm employees about parasitic otitis and practices regarding methods of treatment and control. A questionnaire was applied on specific topics about the disease. The results showed that the otitis occurred in 25 (89.3%) of the 28 farms studied, where the main etiologic agents were the nematodes Rhabditis spp. The majority of the farmers regarded the disease as a serious problem, mainly in cattle older than eight years of age. About half of the respondents did not observe a correlation between season and prevalence of the otitis; however, 30.8% reported an increase of its incidence during the rainy season. In addition to various methods and products for the rhabditiosis treatment, a lack of standardized preventive or curative protocols was found, suggesting insufficient knowledge about control of the disease.


Este estudo objetivou conhecer a percepção de criadores e responsáveis técnicos de rebanhos bovinos da raça Gir frente à otite parasitária, inclusive suas práticas relativas aos métodos de controle e tratamento. Utilizou-se um questionário contendo tópicos específicos relacionados à doença. Os resultados mostraram que a otite esteve presente em 25 (89,3%) das 28 propriedades estudadas, sendo os nematóides do gênero Rhabditis o principal agente etiológico encontrado. A maioria dos entrevistados considerou a doença como um sério problema do rebanho, ocorrendo principalmente nos animais com mais de oito anos de idade. Cerca de metade dos entrevistados não observaram correlação entre a época do ano e maior prevalência da doença, por sua vez, 30.8% deles relataram o aumento da frequência de casos de otite durante a estação chuvosa. Verificou-se, além da grande variedade de métodos e produtos para o tratamento da rhabditiose, a ausência de padronização dos protocolos preventivos ou curativos, sugerindo conhecimento insuficiente a respeito do seu controle.


Subject(s)
Animals , Cattle , Diptera , Health Surveys , Otitis/veterinary , Rhabditoidea/isolation & purification , Insect Vectors , Signs and Symptoms/veterinary
9.
Mem. Inst. Oswaldo Cruz ; 106(4): 433-440, June 2011. ilus
Article in English | LILACS | ID: lil-592185

ABSTRACT

The nematode parasites of Rhinella marina include species of the genus Rhabdias (Rhabdiasidae: Rhabditoidea). The present study describes Rhabdias paraensis sp. nov., which parasitizes the lungs of R. marina in Brazilian Amazonia. Of the more than 70 known species of this genus, 18 are parasites of bufonids, of which, eight are Neotropical. The new species described here is similar to Rhabdias alabialis in the absence of lips is different by the presence of conspicuous cephalic papillae. We describe details of the four rows of pores, which are distributed equally along the whole of the length of the body and connected with hypodermal cells, using histology and scanning electron microscopy. Other histological aspects of the internal structure of this nematode are also described.


Subject(s)
Animals , Female , Bufonidae , Lung , Rhabditoidea , Microscopy, Electron, Scanning , Rhabditoidea/ultrastructure
10.
Rev. MVZ Córdoba ; 15(3): 2240-2244, sept.-dic. 2010.
Article in Spanish | LILACS | ID: lil-621916

ABSTRACT

Se reportan dos casos de bovinos de la raza Gyr puros, atendidos en el servicio ambulatorio de la clínica medico-quirúrgica de grandes animales de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad de Córdoba, Colombia. Los animales presentaron al examen físico signos de otitis clínica en ambos lados, con otorrea, olor fétido, inflamación con cierto grado de estenosis del conducto auditivo, inclinación de la cabeza, prurito intenso y rascado excesivo de las orejas. Se realizó inspección directa y colecta de material del conducto auditivo externo, y se observó en forma directa el movimiento de los parásitos en el cerumen, posteriormente confirmado por observación microscopio-estereoscópico, en el laboratorio de parasitología de la Facultad de Medicina Veterinaria de la Universidad de Córdoba, en el cual se reveló la presencia de larvas y adultos de nemátodos del género Rhabditis bovis. Es el primer reporte de esta enfermedad en bovinos de la raza Gyr en el departamento de Córdoba y Colombia.


Subject(s)
Cattle , Cattle , Otitis , Rhabditoidea
11.
Pesqui. vet. bras ; 29(11): 910-912, Nov. 2009. tab
Article in English | LILACS | ID: lil-539041

ABSTRACT

The purpose of this study was to evaluate the efficacy of orally administered albendazole sulphoxide and pour-on ivermectin for the treatment of bovine parasitic otitis caused by rhabditiform nematodes. Eighteen Gyr cows presenting clinical otitis were divided in three groups with six animals each. The first one did not receive any treatment (control group). The second one was treated with 0.5 percent pour-on ivermectin, 500µg/kg of body weight, and the third group was treated with oral 6 percent albendazole sulphoxide, at 6.0mg/kg. Both ear canals of each animal were reexamined on days 7 and 21 post treatment. The animals in the control group remained infected throughout the days of observation. Ivermectin treatment did not show effectiveness on days 7 or 21 post treatment. The albendazole sulphoxide treatment had an efficacy of 16.7 and 25 percent on days 7 and 21, respectively. Further studies are required to assess an effective treatment for this parasitic disease, especially via alternative administration routes, because of its significant impact on Bos taurus indicus cattle breeding in Tropical and Subtropical Regions.


O objetivo deste estudo foi avaliar a eficácia do sulfóxido de albendazol administrado oralmente e da ivermectina "pour-on" no tratamento da otite parasitária bovina causada por nematóides rhabditiformes. Dezoito vacas Gir apresentando otite clínica foram divididas em três grupos de seis animais cada. O primeiro não recebeu tratamento (grupo controle). O segundo foi tratado com ivermectina "pour-on" a 0,05 por cento na dose de 500µg/kg de peso vivo. O terceiro grupo foi tratado com sulfóxido de albendazol oral a 6 por cento na dose 6,0mg/kg. Os condutos auditivos de todos os animais foram reexaminados nos dias 7 e 21 pós-tratamento. Os animais do grupo controle permaneceram infectados nos dias de observação. O tratamento com ivermectina não demonstrou eficácia alguma para os dias 7 e 21 pós-tratamento. O tratamento com sulfóxido de albendazol obteve 16,7 e 25 por cento de eficácia nos dias 7 e 21, respectivamente. Mais estudos são necessários para determinação de tratamentos eficazes para tal doença parasitária, especialmente através de vias alternativas de administração, por causa de seu significante impacto na criação de Bos taurus indicus nas Regiões Tropical e Subtropical.


Subject(s)
Animals , Female , Cattle , Albendazole/administration & dosage , Parasitic Diseases, Animal/prevention & control , Ivermectin/administration & dosage , Otitis/veterinary , Rhabditoidea/parasitology , Administration, Oral , Anthelmintics/adverse effects
12.
Journal of Qazvin University of Medical Sciences and Health Services [The]. 2009; 12 (4): 83-89
in Persian | IMEMR | ID: emr-91852

ABSTRACT

Outbreaks of human parasitic diseases associated with the consumption of raw vegetables, often occur in developing and developed countries. To evaluate parasitic contamination of edible vegetables in Qazvin. This was a descriptive analytical study in which 150 samples of different vegetables including leek, parsley, lettuce, coriander, radish, spring onion, tarragon, basil, mint, and cress; collected from several greengroceries in Qazvin during 12 months between 2006-2007 were examined. Samples were evaluated for presence of metazoan and protozoan parasitic contaminations. The vegetable samples were washed with water and tested microscopically after performance of sedimentation method. Fifty three out of 150 samples [35.3%] were found to have parasitic contamination. Among those 45 [30%] and 8 [5.3%] were revealed to be metazoa and protozoa, respectively. Metazoan contaminations were detected by observation of helminthes' eggs 13 [8.6%] and rhabditoid larva 32 [21.4%] in vegetables. The highest rate of contamination was detected in leek [60%], and the lowest in coriander and radish [20%]. Regarding the parasitic infestation, rhabditoid larva with 21.4% and Hymenolepis nana ova with 0.7% were shown to have the highest and lowest rates, respectively. Based on our results, edible vegetables in Qazvin are the potential sources of several parasitic infections in human


Subject(s)
Vegetables , Developing Countries , Developed Countries , Food Contamination , Eukaryota , Onions , Petroselinum , Lactuca , Coriandrum , Raphanus , Mentha , Helminthiasis , Rhabditoidea , Larva , Hymenolepis nana
13.
The Korean Journal of Parasitology ; : 13-17, 2009.
Article in English | WPRIM | ID: wpr-53285

ABSTRACT

Dung beetles (family Scarabaeidae) are one of the largest families of beetles worldwide. Due to biological behavior of these arthropods, they are considered to play an important role in the life cycle of some helminths. In the present study, dung beetles collected from cattle pastures in rural areas of Ardabil province, north-west of Iran were examined for infection with larval stages of helminths. According to the results, nematodes of 2 genera were identified including Rhabditis and Gongylonema. The more common species was Rhabditis sp. which was found in 9 species of beetles. Out of 15 different species of dung beetles, Copris lunaris was the only scarabaeid to be found naturally infected with the larval stages of Gongylonema sp. Our new findings introduce C. lunaris as a potential biological vector for transmission of Gongylonema sp. to vertebrates in the surveyed region.


Subject(s)
Animals , Humans , Arthropod Vectors/parasitology , Coleoptera/parasitology , Iran/epidemiology , Larva/physiology , Rhabditoidea/isolation & purification , Spirurida Infections/epidemiology , Spiruroidea/isolation & purification
14.
Iranian Journal of Parasitology. 2008; 3 (3): 42-47
in English | IMEMR | ID: emr-99449

ABSTRACT

We describe a 61 -year-old man who developed a mild gastrointestinal discomforts including diarrhea associated with feces containing many larvae similar to rhabditiform larvae of Strongyloides stercoralis diagnosed finally as Diploscapter coronata, a free-living nematode. This case is unusual infection in human and there are a few reports of this worm as a real parasite. Microscopically, Diploscapter larvae in feces should be considered in the differential diagnosis of Strongyloididasis and hook worm disease in endemic area. This was the first reported occurrence of human D. coronata infection in Iran. Most of the clinical manifestations of human infection are not yet known due to limited information; thus, there is a need for more studies


Subject(s)
Humans , Male , Signs and Symptoms, Digestive , Rhabditoidea/isolation & purification , Rhabditoidea , Review Literature as Topic , Host-Parasite Interactions , Parasite Egg Count , Soil/parasitology , Strongyloides stercoralis , Larva , Diarrhea
15.
Southeast Asian J Trop Med Public Health ; 2005 ; 36 Suppl 4(): 99-101
Article in English | IMSEAR | ID: sea-33343

ABSTRACT

A 73-year-old Thai woman living in Mueang District, Saraburi Province, central Thailand presented with numerous hookworm-like nematodes, finally revealed as Diploscapter coronata, by fecal culture. The patient exhibited no significant clinical signs of the gastrointestinal or genitourinary systems, and was generally not ill as a result of this unusual infection. Less commonly, patients have presented with symptoms and signs of Diploscapter coronata infection. However, potentially serious consequences can occur where people have exposure to an environment that has been contaminated with infected feces, or more specifically, infective eggs; such conditions could lead to human infection with Diploscapter coronata worms. This was the first reported occurrence of human Diploscapter coronata infection in Thailand.


Subject(s)
Aged , Animals , Feces/parasitology , Female , Food Contamination , Humans , Rhabditida Infections/diagnosis , Rhabditoidea/isolation & purification , Soil/parasitology , Thailand
16.
Indian J Exp Biol ; 2003 Apr; 41(4): 376-8
Article in English | IMSEAR | ID: sea-60842

ABSTRACT

Mustard sawfly, A. lugens proxima, was found to be highly susceptible to entomopathogenic nematode, S. feltiae under laboratory condition. Application of three different doses of S. feltiae, viz. 1.1 x 10(3), 1.1 x 10(4) and 1.1 x 10(5) infective juveniles/ml, at weekly intervals, significantly reduced the field population of mustard sawfly on radish. The mean larval population of A. lugens proxima in all doses of nematode treated plots ranged from 0.42 to 0.48 larvae per plant as against 2.95 larvae / plant in untreated control plots. Similarly, the yield of radish in all the nematode treated plots was significantly higher by way of recording 2.80 to 2.87 tons/ha as compared to 1.63 tons/ha in the case of control.


Subject(s)
Animals , Host-Parasite Interactions , Hymenoptera/growth & development , Pest Control, Biological , Raphanus/growth & development , Rhabditida Infections , Rhabditoidea/pathogenicity
17.
Rev. Soc. Bras. Med. Trop ; 35(5): 519-522, Sept.-Oct. 2002. ilus
Article in Portuguese | LILACS | ID: lil-328005

ABSTRACT

Relata-se um caso de parasitismo por Rhabditis sp em criança com cinco meses de idade procedente do Estado de Goiás, Brasil. O quadro clínico mostrou inicialmente diarréia com fezes líquidas esverdeadas e posteriormente sanguinolentas. O exame parasitológico de fezes revelou a presença de larvas e adultos de Rhabditis sp. Após o uso de thiabendazole houve melhora no quadro clínico e cura. Os autores chamam atençäo para a importância de se estabelecer o diagnóstico diferencial entre Strongyloides e Rhabditis


Subject(s)
Animals , Female , Humans , Infant , Male , Feces/parasitology , Rhabditida Infections/diagnosis , Rhabditoidea/isolation & purification , Antinematodal Agents/therapeutic use , Diagnosis, Differential , Rhabditida Infections/drug therapy , Rhabditoidea/anatomy & histology , Thiabendazole/therapeutic use
18.
Rev. Inst. Adolfo Lutz ; 33(1-2): e37041, dez.28,1973. ilus
Article in Portuguese | LILACS, ColecionaSUS, SES-SP, CONASS, SESSP-IALPROD, SES-SP, SESSP-IALACERVO | ID: biblio-1066533

ABSTRACT

São apresentados 3 casos nos quais foram encontrados, em fezes humanas, vermes adultos da superfamília Rhabditoidea, sendo dois do gênero Strongyloides e um do gênero Rhabditis (AU).


Subject(s)
Humans , Male , Female , Feces , Rhabditoidea , Strongyloides stercoralis
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