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Journal of Forensic Medicine ; (6): 174-176, 2005.
Article in Chinese | WPRIM | ID: wpr-983101

ABSTRACT

OBJECTIVE@#To probe the correlation between the postmortem interval (PMI) and the DNA degradation of costicartilage and dental pulp cells in human being after death, and to seek a new method for estimating PMI.@*METHODS@#The image cytometry was used to measure the DNA degradation under different ambient temperatures (30-35 degrees C, 15-20 degrees C) in 0-15 days after death.@*RESULTS@#The average DNA content of two kinds of tissue was degradated with the prolongation of PMI. But there was a plateau period of 0-4 days for dental pulp cells of human being in 15-20 degrees C. There was a high negative correlativity P<0.01 between the average DNA content and PMI.@*CONCLUSION@#PMI could be estimated accurately according to the DNA degradation of costicartilage and dental pulp cells in human being after death.


Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Autopsy , Cartilage/metabolism , DNA/metabolism , Dental Pulp/cytology , Forensic Pathology/methods , Image Processing, Computer-Assisted/methods , Postmortem Changes , Ribs/metabolism , Temperature , Time Factors
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