ABSTRACT
Las rickettsiosis son enfermedades de zonas endémicas quetienen como condicionantes, factores socioeconómicos además de epidemiológicos. Resulta necesario abordar los aspectos determinantes de su presentación en nuestro país, en donde la información esescasa. El presente estudio describe algunos aspectos epidemiológicos relacionados e incursiona por primera vez en el Perú en intentos de caracterización de especies circulantes de rickettsias a partir deaislamientos de humanos y también de vectores procedentes de localidades fronterizas de los departamentos de Tacna, Tumbes, Loreto y Madre de Dios, colectados durante un estudio poblacional previo para detectar prevalencia. OBJETIVOS: Describir algunos aspectos característicos, clínico-epidemiológicos y genómicos de la circulación de rickettsias durante los años 2011 y 2012 en localidades de 4 zonas de frontera del territorio peruano. METODOS: Se analizaron datos clínicos y epidemiológicos de individuos de diversos grupos etarios, información almacenada en 1,657 fichas del estudio previo, el cual contó con aprobación por Comité deÉtica del Instituto Nacional de Salud (CEI-INS) y utilizó consentimiento informado. Se reactivaron ypropagaron aislamientos obtenidos de humanos durante el estudio previo, requiriéndose múltiples pasajes para obtener una carga microbiana aceptable. También fueron usados macerados de vectores (pulgas y garrapatas), obtenidos al momento del estudio, a partir de perros y gatos, en las mismas localidades estudiadas. Se hizo extracción de ADN de los aislamientos y alternativamente de maceradosde vectores, se corrieron pruebas PCR para los genes htrA y gltA. Luego se procedió a purificar los genomas para someterlos a secuenciamiento. Para la parte epidemiológica se evaluó proporciones...
The rickettsial diseases are present in areas with favorableconditions such as socioeconomic and epidemiological characteristics. It is necessary to address thedeterminants of presentation in our country, where information is scarce. This study aims to describesome related epidemiological aspects and attempts to characterize for the first time in Peru thecirculating species of rickettsiae isolated from patients and also from vectors present in fourdepartments: Tacna, Tumbes, Loreto and Madre de Dios, which were collected during previouspopulation-based study to detect its prevalence.OBJECTIVES:Describe some characteristic, clinical-epidemiological and genomic aspects of the circulation ofrickettsias during the years 2011 and 2012 in 4 border areas of Peru.METHODS: We analyzed clinical and epidemiological data from 1,657 individuals of various agegroups. The information was obtained from records stored in a previous study and past and presentstudies received approval by the Ethics Committee of the INS and used the same informed consent. Inaddition, reactivated and propagated isolates from humans and also vectors macerated, obtained duringthe previous study were used to perform DNA extraction and PCR tests for the htrA and gltA gene inorder to proceed the genomic sequencing and identify the isolates...
Subject(s)
Humans , Epidemiologic Studies , Rickettsiaceae Infections/epidemiology , Rickettsia/isolation & purification , Border Areas , Peru , PrevalenceABSTRACT
In this study, rickettsial infection was searched in 108 canine blood samples and 22 Rhipicephalus sanguineus (Ixodida,Ixodidae) ticks collected on these dogs during 2011 and 2012 in Patos municipality, state of Paraíba, northeasternBrazil. Blood samples were tested through indirect immunofluorescence assay (IFA) by using antigens of six Rickettsiaspecies isolates from Brazil. All 108 dogs tested seronegative for R. rickettsii, R. parkeri, R. amblyommii, R. felis, R.rhipicephali, and R. bellii antigens, suggesting a non-endemic status of the studied region for spotted fever ricketsiosis.Among 22 R. sanguineus ticks, R. felis was detected in one (4.5%) specimen by PCR targeting a portion of the rickettsialgltA gene. The possible implications of this unusual PCR finding are discussed.
No presente trabalho, foi pesquisada infecção riquetsial em amostras de sangue de 108 cães e 22 carrapatos Rhipicephalussanguineus (Ixodida, Ixodidae) coletados destes animais durante 2011 e 2012, em Patos, Estado da Paraíba, Nordestedo Brasil. As amostras de sangue foram examinadas pela reação de imunofluorescência indireta (RIFI) utilizando-seantígenos de seis isolados de Rickettsia do Brasil. Todos os 108 cães foram soronegativos para os antígenos de R.rickettsii, R. parkeri, R. amblyommii, R. felis, R. rhipicephali e R. bellii, sugerindo que a região estudada não é endêmicapara riquetsioses do grupo da febre maculosa. Dos 22 carrapatos R. sanguineus, R. felis foi detectada em um (4,5%)espécime por PCR do gene riquetsial gltA. São discutidas as possíveis implicações desse achado incomum na PCR.
Subject(s)
Animals , Dogs , Rickettsiaceae Infections/epidemiology , Rickettsiaceae Infections/blood , Rhipicephalus sanguineus/parasitology , Rickettsia/classification , Polymerase Chain Reaction/veterinary , Fluorescent Antibody Technique, Indirect/veterinaryABSTRACT
A study on tick-borne rickettsiosis was developed in the county of Santa Cruz do Escalvado, State of Minas Gerais, Brazil, where a clinical case of the disease, confirmed by necropsy, had been reported. Of the 1,254 ticks collected 1,061 belonged to the Amblyomma genus, 57 to the Rhipicephalus sanguineus species, 81 to Boophilus microplus, and 46 to Anocentor nitens. The hemolymph test associated with Gimenez staining showed that 18 of the 221 A. cajennense specimens, 1 of the 16 R. sanguineous, 1 of the 22 B. microplus, 3 of the A. nitens, and 1 of the A. ovale contanined rickettsia-like microorganisms. Only 3 A. cajennense ticks were positive under direct immunofluorescence. A. cajennense was the only species found on humans.
Subject(s)
Animals , Rocky Mountain Spotted Fever/epidemiology , Ticks/classification , Ticks/parasitology , Rickettsia rickettsii , Rickettsiaceae Infections/epidemiologyABSTRACT
Cases of Brasilian spotted fever (BSF) have been occurred since 1985, in the rural area of Pedreira town, situated 160 km away from the city of Säo Paulo (longitude 46 degrees 54'07®, latitude 22 degrees 44'21®). Aiming at evaluating the prevalence of Brasilian spotted fever in this endemic area, single-serum samples were collected from 473 healthy persons, amongst city-dwellers and the local china industry workers. The obtained samples were tested by indirect immunofluorescence (IFA), in order to determine the antibodies titer for the group of Brasilian spotted fever. Twenty-five (5.3 per cent) were considered positive (titer 3 1:64) and thirty-one (6.5 per cent) were taken as ®borderline® (titer 1:32). The results show a serologically positivity rate similar to other areas, known as endemic ones within the country.
Subject(s)
Humans , Infant , Male , Middle Aged , Adolescent , Adult , Child , Child, Preschool , Female , Rickettsia rickettsii , Rickettsiaceae Infections/epidemiology , Brazil/epidemiology , Age Distribution , Sex Distribution , Health Surveys , Rural HealthABSTRACT
Se refieren 3 casos autóctonos de rickettsiosis cútaneo ganglionar transmitidos por garrapatas de perros (Amblyomma maculatum, en uno de ellos) en el Uruguay. Dos de los 3 casos fueran seguramente provocados por Rickettsia conorii de acuerdo a los resultados de la rección específica de inmunofluorescencia indirecta-IgM, anti R. conorii. Se incluye un tercer paciente no estudiado con tal técnica, por la similitud clínico-epidemiológica, la reactividad del suero frente al Proteus OX 19 y la rápida respuesta a la tetraciclina. La no descripción previa de la rickettsiosis por R. conorii en forma autóctona en el área de las américas confiere especial interés a la comunicación, recomendandose la búsqueda de la afección en otros países de la region