ABSTRACT
Salmonella Paratyphi A es un patógeno exclusivo de humanos, siendo la segunda causa más común de fiebre entérica en el sudeste asiático. Recientemente la incidencia en este continente ha aumentado, desplazando a Salmonella entérica serotipo Typhi como la primera causa de fiebre entérica. En la actualidad no existen vacunas licenciadas contra S. Paratyphi A. El Instituto Finlay de Vacunas se encuentra trabajando en la obtención de un candidato vacunal basado en vesículas de membrana externa (VME) contra S. Paratyphi A, por lo que se hizo necesario contar con una técnica para la evaluación de su inmunogenicidad. El objetivo de este trabajo fue la estandarización de un ELISA para la cuantificación de anticuerpos IgG contra VME de S. Paratyphi A. Para ello, se determinaron las mejores condiciones de este ensayo en cuanto a concentración óptima de recubrimiento y dilución de trabajo del conjugado. Además, se definió el intervalo y linealidad de la curva, la precisión intra e interensayo, la especificidad y el límite de detección. La curva de calibración se generó con un suero estándar interno y presentó un buen ajuste lineal con un R² =0.98. Los coeficientes de variación en los ensayos de precisión intra e interensayo estuvieron en los intervalos establecidos para cada uno (=10 por ciento, =20 por ciento respectivamente). Los resultados obtenidos avalan el empleo de este ELISA cuantitativo para la evaluación de la inmunogenicidad de formulaciones de VME de S. Paratyphi A en fases de investigación y desarrollo(AU)
Salmonella Paratyphi A, is an exclusive pathogen of humans, being the second most common cause of enteric fever in Southeast Asia. Recently the incidence of this disease in this continent has increased, displacing Salmonella enterica serotype Typhi as the first cause of enteric fever. Currently there are no vaccines licensed against S. Paratyphi A. The Finlay Institute of Vaccines is working on obtaining a vaccine candidate based on outer membrane vesicles (VME) against S. Paratyphi A, so it became necessary to develop a technique for the evaluation of its immunogenicity. The objective of this work was the standardization of an ELISA for the quantification of IgG antibodies against VME of S. Paratyphi A. The best conditions of this assay were determined in terms of optimum concentration of coating and working dilution of the conjugate. In addition, the interval and linearity of the curve, the intra- and inter-assay precision, the specificity and the limit of detection were defined. The calibration curve was generated with an internal standard serum and presented a good linear fit with an R² =0.98. The coefficients of variation in the intra- and interassay precision tests were in the intervals established for each one (=10 percent, =20 percent respectively). The results obtained support the use of this quantitative ELISA for the evaluation of the immunogenicity of VME formulations of S. Paratyphi A in research and development phases(AU)
Subject(s)
Humans , Animals , Salmonella paratyphi A/pathogenicity , Paratyphoid Fever/epidemiology , Salmonella paratyphi A , Enzyme-Linked Immunosorbent AssaySubject(s)
Salmonella paratyphi A/pathogenicity , Salmonella paratyphi A/ultrastructure , Salmonella paratyphi B/pathogenicity , Salmonella paratyphi B/ultrastructure , Salmonella paratyphi C/pathogenicity , Salmonella paratyphi C/ultrastructure , Salmonella typhi/classification , Salmonella typhi/pathogenicity , Salmonella typhi/ultrastructure , Typhoid Fever , Diagnosis, Differential , Paratyphoid Fever , Typhoid-Paratyphoid VaccinesABSTRACT
In a microbiological examination of 70 packed and unpacked samples of ice cream and related products such as kulfi. kulfa, cones and ice cubes collected from different localities of Lahore, the total viable counts ranged from 1.0x10[4]/g to 6.0x10[6]/g. Salmonella occurred in 10 out of 70 samples [14.3%]. The maximum occurrence [75%] of Salmonella was recorded in samples collected from Muslim Town
Subject(s)
Ice Cream , Milk/microbiology , Microbiology , Salmonella paratyphi A/pathogenicity , Salmonella paratyphi B/pathogenicityABSTRACT
Between 1979 and 1989 patients presented with soft tissue infections caused by salmonella typhi or paratyphi B, at the Infectious Diseases Hospital Dr. Lucio Cordova, Santiago, Chile. These complications appeared during or after the clinical course of an enteric fever, or without any antecedent illness. The patients were predominantly young and females. The lesions were mainly located in the lower extremities. 50 percent of the patients presented with a concommitant deep bone or joint infection. 11 patients were treated with cloramphenicol (average 22,8 days), 3 with TMP-SMX (average 23 days) and 4 whith a combination of drugs. 13 patients required surgical drainage. This unusual complication extended hospital stay convelescence, but all our patients had a complete recovery
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Paratyphoid Fever/complications , Soft Tissue Infections/etiology , Typhoid Fever/complications , Clinical Evolution , Salmonella paratyphi A/isolation & purification , Salmonella paratyphi A/pathogenicity , Salmonella typhi/isolation & purification , Salmonella typhi/pathogenicity , Soft Tissue Infections/drug therapy , Soft Tissue Infections/parasitologyABSTRACT
Un recién nacido, hijo de una madre infectada con salmonella paratyphi B, evoluciona en el período neonatal inmediato, con una sepsis por salmonella paratyphi B. El contagio ocurre en el período intrauterino. La sepsis se confirma con hemocultivos (+) en la madre y el hijo al mismo germen
Subject(s)
Humans , Female , Pregnancy , Infant, Newborn , Adult , Bacteremia/congenital , Paratyphoid Fever/diagnosis , Salmonella paratyphi A/pathogenicity , Ampicillin/therapeutic use , Bacteremia/diagnosis , Bacteremia/drug therapy , Bacteremia/etiology , Clinical Evolution , Infectious Disease Transmission, Vertical , Paratyphoid Fever/drug therapy , Paratyphoid Fever/etiology , Salmonella paratyphi A/drug effects , Salmonella paratyphi A/isolation & purification , Hematologic TestsABSTRACT
A case of salmonella paratyphi A fever with reversible pancytopenia in a 15 year old boy who presented with history of high grade continuous fever, epistaxis and haemoptysis, relative bradycardia and splenomegaly is described here. A brief review of the literature on possible causes of reversible pancytopenia in this case is also discussed
Subject(s)
Humans , Male , Salmonella paratyphi A/pathogenicity , Hematologic Tests/methods , Pancytopenia/chemically inducedABSTRACT
Se presenta el caso de una paciente nuligesta de 29 años de edad, portadora de un absceso ovárico puro, de origen tífico, tratado por punción y drenaje por vía laparoscópica. Se discute lo infrecuente de la etiología y se revisa la literatura respecto al manejo endoscópico de colecciones supuradas pelvianas