ABSTRACT
PURPOSE: To compare sperm recovery from slow versus rapid thawing technique using thirty-eight normozoospermic human sperm samples, as follows. Twenty-one samples from men taking part in routine infertility screening exams (infertile group) and seventeen from proven fertile volunteer men with at least one child (fertile group). MATERIALS AND METHODS: After analysis of motility, concentration, strict morphology and functional integrity of membranes, sperm was divided into two aliquots of 0.5 mL each and frozen in TyB-G medium. Samples were thawed at room temperature (25 ± 2º C) for 25 minutes (slow thaw) or in a water bath at 75º C for 20 seconds followed by water bath at 37º C for 3 minutes (rapid thaw). After thawing, motility, strict morphology and functional integrity of membranes were evaluated by a blinded investigator. The results were expressed as mean ± standard deviation for parametric variables and analyzed using Student's t-test. Data with unpaired non-parametric variables were expressed as median (interquartile range) and analyzed by the Mann-Whitney test. Wilcoxon test was used to analyze non-parametric paired variables. RESULTS: There was no significant difference between techniques for total and progressive motility, percentage of normal morphological forms, hypoosmotic swelling test. CONCLUSIONS: Although the rapid thawing protocol was completed in a shorter time (three minutes and 20 seconds versus 25 minutes, respectively), it wasn't harmful since both techniques showed comparable spermatozoa recovery. Additional research is needed to confirm its safety in clinical research before introducing this methodology in routine assisted reproduction.
Subject(s)
Adult , Humans , Male , Cryopreservation/standards , Fertility/physiology , Infertility, Male/physiopathology , Semen Preservation/standards , Sperm Motility/physiology , Spermatozoa/physiology , Cryopreservation/methods , Double-Blind Method , Sperm CountABSTRACT
Se presentan 4 casos de embarazos en 12 ciclos de inseminación intrauterino usando semen seleccionado criopreservado en Banco de Semen
Subject(s)
Humans , Biological Specimen Banks/standards , Semen Preservation/standards , Insemination, Artificial, Heterologous/methods , Cryopreservation/methods , Infertility/therapy , Sexually Transmitted Diseases/prevention & control , Tissue Donors/supply & distributionABSTRACT
O objetivo do presente trabalho foi avaliar a eficiência de sêmen congelado, submetido às provas rápida (45 §C/1 hora) e lenta (38 §C/5 horas) de termo-resistência, sobre a fertilidade de fêmeas da espécie bovina. Cento e dezenove vacas foram selecionadas e divididas em 2 tratamentos. No tratamento 1 foram incluídos 6 grupos de 10 fêmeas, inseminadas com material cuja análise laboratorial revelou motilidade espermática progressiva superior ou igual a 20//, na prova rápida e valor menor a 20//na prova lenta. O tratamento 2 (controle) utilizou 59 vacas distribuídas em 5 grupos de 10 e um grupo de 9, inseminadas com material que, na prova lenta, apresentou 20//de motilidade espermática progressiva e qualquer valor na prova rápida. Apenas uma palheta foi utilizada por estro, tolerando-se até 3 repetiçöes de cio. O diagnóstico de gestaçäo foi realizado por palpaçäo retal, cerca de 60 dias após a última inseminaçäo artificial. Resultados satisfatórios foram alcançados nas taxas de prenhez (TTR/R = 0,793 ñ 0,05 e TTR/L = 0,875 ñ 0,05), näo havendo diferença estatística (P>0,05) quanto ao tipo de provas do termo-resistência. Näo houve diferença significativa (P>0,05) de prenhez de fêmeas inseminadas com material submetido ao teste de termo-resistência rápido, quando a motilidade espermática variou de 20 a 50//