ABSTRACT
A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geographic origin.
Subject(s)
Genotype , Polymerase Chain Reaction , Methods , Random Amplified Polymorphic DNA Technique , Methods , Sequoia , Classification , GeneticsABSTRACT
Recurrent episodes of venoocclusive priapism have been described previously in patients who have had repeated exposure to a recognized stimulus such as intracavernosal injections of vasoactive agents, or pathologic conditions such as spinal stenosis or hematological disorders. Three patients were referred for evaluation of an unusual sequoia of an initial episode of idiopathic venoocclusive priapism, occurred with a frequency ranging from several times per month to once per year and were symptomatically disabling. We reviewed the characteristics and the possible pathophysiology of this syndrome and some therapeutic options in the management of these patients.
Subject(s)
Humans , Priapism , Sequoia , Spinal StenosisABSTRACT
While in recent there has been a worldwide decrease in the incidence of convulsion Iron toxemic pregnancy due to improved in the quality and quantity of prenatal care, there has been a relative increase in the proportion of postpartum cases of eclampsia. Postpartum eclampsia accounts for 25% of total eclampsia and occurs most frequently during the first postpartum day. We experienced a case of postpartum eclampsia of immediate onset after cesarean section in a 29 year eld primigravida patient with mild preeclampsia antenataly. She had two convulsive seigure, the first attacH occured juat before the end of anesthesia and the second attacik about 30 minutes after the cesarian section. At that time the patient was treated with thiopental and diazepam intravenously Under the impression of postpartum eclampsia she was given MgSO4, for one day and experienced no other seizures after then. She awakened fully and exhibited no neurological sequeale from the eclampsia. She was discharged from the hospital on the sixth postoperative day with no other complications or sequoia.
Subject(s)
Female , Humans , Pregnancy , Anesthesia , Cesarean Section , Diazepam , Eclampsia , Incidence , Iron , Postpartum Period , Pre-Eclampsia , Prenatal Care , Seizures , Sequoia , ThiopentalABSTRACT
While in recent there has been a worldwide decrease in the incidence of convulsion Iron toxemic pregnancy due to improved in the quality and quantity of prenatal care, there has been a relative increase in the proportion of postpartum cases of eclampsia. Postpartum eclampsia accounts for 25% of total eclampsia and occurs most frequently during the first postpartum day. We experienced a case of postpartum eclampsia of immediate onset after cesarean section in a 29 year eld primigravida patient with mild preeclampsia antenataly. She had two convulsive seigure, the first attacH occured juat before the end of anesthesia and the second attacik about 30 minutes after the cesarian section. At that time the patient was treated with thiopental and diazepam intravenously Under the impression of postpartum eclampsia she was given MgSO4, for one day and experienced no other seizures after then. She awakened fully and exhibited no neurological sequeale from the eclampsia. She was discharged from the hospital on the sixth postoperative day with no other complications or sequoia.