ABSTRACT
Filariasis is one of the typical parasitic infections which cause immune suppression during the course of infection in both humans and experimental animals. A 29 kDa protein isolated from detergent soluble antigen of S. digitata showed maximum inhibition of cell mediated immune response. The heat inactivated 29 kDa protein was found to be devoid of property of suppression of immune response in the host. Histological study of spleen of BALB/C mice immunized with 29 kDa protein showed changes in regions of spleen such as follicle, trabeculae, capsule, reticuloendothelial cells and eosinophils. The 29 kDa protein, the most reactive of the detergent soluble proteins produced partial suppression of immune response, thereby contributing to the factors responsible for the survival of filarial parasites in hosts.
Subject(s)
Animals , Antigens, Helminth/chemistry , Humans , Immune Tolerance , Mice , Mice, Inbred BALB C , Molecular Weight , Setaria Nematode/immunology , Setariasis/immunology , Solubility , Spleen/immunologyABSTRACT
Certain excretory/secretory proteins released by adult females of the bovine filarial parasite, Setaria digitata, along with the release of microfilariae when chromatographically analysed has three major protein fractions of molecular weights 70 kD (ESF1), 16.5 kD (ESF2) and 11 kD (ESF3). Of these ESF2 and ESF3 cross reacted with antibodies from Wuchereria bancrofti infected humans. ESF2 was more specific and accurate in detecting human filarial infection. Similar proteins secreted by human filarial parasites could be targets for combating the disease by cure or control.
Subject(s)
Animals , Antibodies, Helminth/immunology , Cattle , Cross Reactions , Elephantiasis, Filarial/immunology , Female , Filarioidea/immunology , Humans , Setariasis/immunology , Wuchereria bancrofti/immunologyABSTRACT
Los antígenos de Setaria cervi hembra son más eficaces que los de macho para evitar el desarrollo de microfilaremia en cabayos (10 parásitos por individuo). Con dosis altas (2ml 500 µl) se obtuvo resistencia significativa a partir de antígenos de parásitos machos (52% de protección) hembras (62%). Los antígenos de hembra tambíen protegieron (40% con dosis bajas (2ml 50 µ/ml)