Identificación de especies de candida en pacientes con periodontitis apicales crónicas no supurativas / Identification of candida species in patientes with chronic apical periodontitis non suppurative

Identificar especies de Cándida en pacientes con diagnóstico de periodontitis apicales crónicas no supurativas. Se realizó un estudio descriptivo in vivo. La población fueron los pacientes con diagnóstico de periodontitis apical crónica no supurativa, que asisten a la facultad de odontología Universidad de Cartagena. Se seleccionó una muestra de 34 conductos, a los cuales se recolectó una muestra del fluido del conducto con puntas de papel, bajo condiciones de asepsia necesarias para este procedimiento. Las cepas fueron aisladas en Agar dextrosa Sabouraud e identificadas por métodos micológicos convencionales y bioquímicos. Del total de las 34 cepas estudiadas, 26 (76,5%) fueron negativas para la presencia de hongos, las 8 muestras restantes fueron positivas, lo que corresponde al 23,5 %. En la determinación de la especie, los resultados fueron positivos para Candida albicans (C. albicans) en un 17,7 % y un 5,9 % para Candida tropicalis (C. tropicalis). La presencia de especies micóticas en patologías periapicales crónicas fue positiva, prevaleciendo la C.albicans. Es importante la identificación de estos agentes en casos de periodontitis apicales crónicas no supurativas, para determinar protocolos que tengan efectividad sobre agentes micóticos.

To identify species of Candida in patients with chronic suppurative apical periodontitis. A descriptive study in vivo. The population were patients diagnosed with chronic apical suppurative periodontitis, attending dental school, University of Cartagena. A sample of 34 ducts, which collected a sample of fluid from the canal with paper points, under aseptic conditions necessary for this procedure. The strains were isolated on Sabouraud dextrose agar and identified by conventional mycological and biochemical methods. A total of 34 strains studied, 26 (76.5%) were negative for the presence of fungi, the remaining 8 samples were positive, corresponding to 23.5%. In determining the species, the results were positive for C. albicans in 17.7% and 5.9% for C. tropicalis. The presence of fungal species in chronic periapical pathology was positive with C. albicans prevailing. It is important to identify these agents in cases of chronic suppurative apical periodontitis, to establish protocols or medicaments that will be effective against fungal agents.

Mycobacterial skin infections: comparison between histopathologic features and detection of acid fast bacilli in pathologic section.

BACKGROUND: Detection of acid fast bacilli (AFB) in chronic granulomatous inflammation is an important clue for mycobacterial infection. DESIGN: A retrospective review of 104 pathologic sections (from 1994 to 2001) of suspected cases of mycobacterial (tuberculous and nontuberculous) skin infections to study histopathologic features and the correlation with the presence of AFB in the section was performed. RESULTS: All cases showed granulomatous inflammations that can be categorized into 4 types: mixed cell, suppurative, tuberculoid and palisading granuloma. AFB was found in 32 sections (30.77%). Ninety five specimens from 104 specimens were simultaneously cultured. AFB positive cases yielded higher positive cultural results, 17 from 29 cases (58.62%) compared to the AFB negative group, 23 from 66 cases, (34.85%). Mixed cell granuloma was the most common histologic feature, but suppurative granuloma was the most common histological feature (56.25%) in which AFB could be found, which was statistically significantly different from other types of granuloma. Tuberculoid granuloma was more common in the AFB negative group (20.83%) compared to the AFB positive group (9.37%) but the difference was not statistically significant. In cases that AFB could not be found, the inflammation tended to be located in the upper half of the dermis. CONCLUSION: AFB can be more frequently detected in suppurative granuloma that might be located in any portion of the dermis. This finding was not species specific.