ABSTRACT
Background: Syphilis is still a public health problem in Latin America and the Caribbean. Therefore maternal screening is of great importance for public health. Aim: To estimate the prevalence of maternal syphilis and to evaluate a rapid diagnostic test for thedisease among Bolivian women. Material and methods: A total of 489 women delivering in a Public Hospital in Cochabamba, Bolivia, answered a questionnaire about socioeconomic andreproductive characteristics and access to health care services. Syphilis was diagnosed by using RPR and FTA-ABS tests. The rapid test Determine syphilis TP was evaluated taking as reference FTA-ABS test. Results: Twenty two women (4.5%) were positive both for RPR and FTA-ABS tests and fifty (10.2%) were positive for the rapid diagnostic test and FTA-ABS. Compared with FTA-ABS, the sensitivity and specificity of the rapid test were 98% and 99.8%, respectively. Only 15% of women that had at least one antenatal care visit had a syphilis screening test and 40% of the women in whom the test was performed, did not receive its results. Increasing age, low socioeconomic level and a history of a sexually transmitted disease were independently associated with syphilis infection. Conclusions: The magnitude of syphilis infection among the studied women was still high in Cochabamba. The rapid test showed excellent overall performance for maternal syphilis screening. Few women of all attended in the hospital had a screening syphilis test and half of those screened were not aware of the results. Maternal syphilis affected older women of low socioeconomic level and with a history of a sexually transmitted disease.
Subject(s)
Adolescent , Adult , Female , Humans , Pregnancy , Young Adult , Syphilis Serodiagnosis/standards , Syphilis/diagnosis , Syphilis/epidemiology , Bolivia/epidemiology , Epidemiologic Methods , Socioeconomic Factors , Syphilis Serodiagnosis/methods , Young AdultSubject(s)
Humans , Bacteria , Bacterial Growth , Bacterial Infections , Blood Component Removal , Blood Donors , Infection Control , Leukapheresis/methods , Serologic Tests/methods , Serologic Tests , Platelet Transfusion/adverse effects , Blood Transfusion/adverse effects , Blood Transfusion/standards , Bacteremia , Environmental Pollution , Gram-Negative Bacteria , Gram-Positive Bacteria , Lyme Disease , Phagocytosis , Risk Factors , Sepsis , Syphilis Serodiagnosis/standards , Syphilis/epidemiology , Syphilis/transmission , Spirochaetales InfectionsABSTRACT
Contiene: 1. Obtención de la muestra de sangre; 2. Prueba del laboratorio de investigaciones en enfermedades venéreas; 3. Prueba de reagina plasmática rápida; 4. Microhemaglutinación para treponema pallidum; 5. Anticuerpos de Treponema Fluorescentes absorbidos; 6. Flujograma de muestras para investigar sifilis; 7. Bioseguridad
Subject(s)
Male , Female , Humans , Syphilis Serodiagnosis/standards , Clinical Laboratory Techniques/standards , PeruABSTRACT
Se realizó un estudio descriptivo prospectivo en el que se valoró la técnica de ELISA (Captia Syphilis G, Mercia Diagnostic, Guilford, England) para detectar anticuerpos anti-Treponema pallidum de la clase inmunoglobulina G. El antígeno utilizado fue extracto protéico de T. pallidum perteneciente a la cepa Nichols. Para comparar la ELISA Captia Syphilis G se utilizó la técnica de inmunofluorescencia indirecta FTA-Abs. Se evaluaron 100 sueros de pacientes cuya distribución fue: 60 sueros de pacientes enfermos de sifilis, 20 sueros de paciente sanos sin enfermedades de trasmisión sexual conocida, 15 sueros de pacientes con enfermedades del colágeno y 5 sueros de pacientes con tuberculosis. La sensibilidad de ELISA Captia Syphilis G fue del 98.4 por ciento mientras que FTA-Abs fue del 100 por ciento. La especificidad de ELISA Captia Syphilis fue del 100 por ciento y del FTA-Abs del 97.3 por ciento. Esta técnica podría ser incluida en Colombia como prueba confirmatoria para el diagnóstico de sífilis por su alta sensibilidad y especificidad, sumado a la facilidad de su realización, sencillez del procedimiento y economía, que ofrece resultados objetivos y confiables
Subject(s)
Humans , Syphilis Serodiagnosis/standards , Syphilis Serodiagnosis/trends , Syphilis Serodiagnosis , Enzyme-Linked Immunosorbent Assay/standards , Enzyme-Linked Immunosorbent Assay/trends , Enzyme-Linked Immunosorbent Assay/statistics & numerical dataABSTRACT
Durante seis meses, os laboratórios de saúde localizados em Florianópolis, Santa Catarina, foram submetidos a uma avaliaçäo externa de qualidade. Esta avaliaçäo constou da análise da concordância de resultados de exames executados para o diagnóstico da sífilis, pela técnica do VDRL. Os resultados foram comparados com os padröes, sendo os laboratórios classificados segundo a perfomance apresentada. Foi também realizado um levantamento em todos os laboratórios e analisados a formaçäo do pessoal, equipamentos e outras condiçöes que poderiam influir, direta ou indiretamente, na qualidade dos exames. A expectativa de que, na avaliaçäo destes laboratórios, fossem encontrados resultados satisfatórios na qualidade dos exames näo se concretizou, havendo deficiências a serem corrigidas. Considerando-se a determinaçäo da percentagem de concordância nos exames qualitativos pelo VDRL nestes laboratórios e a análise das variáveis intervenientes nos resultados, sugere-se a implantaçäo de programas de controle de qualidade interno e de avaliaçäo de qualidade externa