Determination of TTX in biological samples by LC-MS/MS / 法医学杂志

OBJECTIVE@#To propose a method for determination of tetrodotoxin (TTX) in human blood, urine and liver by liquid chromatography with tandem mass spectrometry (LC-MS/MS).@*METHODS@#Solid phase extraction is used after the samples are precipitated, then the samples will be analyzed by LC-MS/MS.@*RESULTS@#The limits of detection were 2 ng/mL in blood and urine and 4 ng/g in liver for TIX respectively, the linear correlation coefficients were not less than 0.9973, both of the intra-day and inter-day precisions were less than 12.80%, the recoveries for all kinds of specimens were more than 47.2%.@*CONCLUSION@#This method is efficient, sensitive and accurate and was successfully validated for implementation in forensic toxicological analysis.