ABSTRACT
Candida albicans, one of the most dreadful fungal pathogens threatening humans, could not be easily prevented. The anticandidal activity of oak gall extract, Quercus infectoria (QIE), was investigated as a potential natural alternative to synthetic and chemical fungicides. QIE anticandidal potentiality was confirmed using both qualitative and quantitative assays. Cotton textiles were treated with QIE and then evaluated as anticandidal fabrics. QIE-treated textiles had a potent anticandidal activity, which could completely inhibit the inoculated C. albicans cells. The durability of anticandidal activity in QIE-treated textiles almost completely disappeared after the fourth laundering cycle. QIE could be recommended, however, as a potent anticandidal agent for preparing antiseptic solutions and emulsions and as a finishing agent for manufacturing anticandidal disposable diapers and hygienic clothes.
Candida albicans es uno de los patógenos fúngicos más terribles que amenazan la salud humana, y su prevención no resulta sencilla. En este trabajo se investigó la actividad anticandidiásica del extracto de agallas de roble (Quercus infectoria extract; QIE) como una posible alternativa natural a los fungicidas sintéticos y químicos. El potencial anticandidiásico del QIE se confirmó mediante análisis cualitativos y cuantitativos. Se trató tejido de algodón de uso textil con QIE y se lo evaluó como tela anticandidiásica. Se verificó que dichos tejidos exhibían una potente actividad anticandidiásica y que podían inhibir completamente a células de C. albicans inoculadas. La actividad anticandidiásica, sin embargo, desapareció por completo después del cuarto ciclo de lavado. Se concluye que se podría recomendar QIE como un agente anticandidiásico potente para la preparación de soluciones antisépticas y emulsiones, y como un agente de acabado para fabricar pañales desechables y ropa de higiene con propiedades anticandidiásicas.
Subject(s)
Antifungal Agents/pharmacology , Cotton Fiber , Candida albicans/drug effects , Plant Extracts/pharmacology , Quercus , Textiles/microbiologyABSTRACT
In the State of Rio Grande do Sul (RS), Southern Brazil, a good manufacturing practices regulation was published recommending two disinfection methods for cleaning cloths used in food services. The aim of the present study was to evaluate the efficacy of those methods. Cleaning cloths were sampled without prior notice at food services, on common working days. For the analyses, the cloths were divided in two sub-samples, being one of them microbiologically analyzed. The second sub-sample was further divided in two pieces and submitted to hand washing for two minutes. After that, one piece was boiled in water for 15 min and the other one was soaked in a 200 ppm sodium hypochlorite solution for 15 min. Both pieces of cloth were submitted to microbiological analyses. Cleaning cloths presented total aerobic mean counts of 6.9 ± 6.7 log/cm². All cleaning cloths presented coliform contamination, and 40% demonstrated mean counts of 6.2 ± 5.6 log/cm². Presumptive S. aureus mean counts of 5.5 ± 4.9 log/cm² were found. No statistic correlation was observed among the number of meals served daily in the food services and the microbiological contamination levels. After washing and disinfection, microbiological counts were significantly (p < 0.05) reduced by both methods, achieving an approximately 5 log reduction. The reductions achieved by the sodium hypochlorite soaking method and the boiling method were not significantly different. Thus, it was possible to conclude that both recommended methods were suitable to disinfect cleaning cloths used in food services.