Método analítico para la determinación de protirelina por cromatografía líquida de alta resolución / Analytical methods for the determination of protirelin by high pressure liquid chromatography

Se desarrolló un método analítico para el control de la calidad y estudio de estabilidad de protirelina inyectable. Se realizó el análisis por cromatografía líquida de alta resolución con la utilización de un sistema isocrático de buffer fosfato-metanol (85:15) como fase móvil y una columna Lichrosorb RP-18 de 250 x 4 µm y detección ultravioleta, a una longitud de onda de 210 nm. El método analítico resultó ser lineal, preciso, exacto y específico en el rango de concentraciones estudiadas

diagnosis of euthyroid graves disease

Twenty patients complaining of eye symptoms, nervousness, palpitation and fatigue and suspected of having grave's disease were diagnosed as euthyroid grave's disease by laboratory data. Antithyroid antibodies and an abnormal TSH response to exogenous TRH were demonstrable denoting abnormalities of the hypothalamo-hypophyseal thyroid axis

TSH ultra-sensible et test a la TRH dans l'insuffisance ante-hypophysaire

In 74 patients with untreated pituitary insufficiency, the TRH- test [Ultra- Sensible TSH performed by immunoradiometric assay] has permitted to detect 48 thyreotrop deficiency. There is no correlation between basal TSH- us values and TSH reponse [TSH] under TRH; only 4 patients with pituitary hypothyroidism presented low basal TSH- us [<0.26 micro Ul/ml]. In the pituitary pathology, the TSH reponse under TRH remains indispensable for the evaluation of TSH pituitary reserves, since basal TSH- us value doesn't permit to predict the reponse TSH to TRH