ABSTRACT
Numerous studies have demonstrated that Radix Astragali can inhibit gastric ulcers in mice. Anhydrous ethanol (0.01 mL/g) administered to mice by intragastric infusion can induce gastric ulcer injury. This study was performed to compare the stomach tissue distribution profiles of four major bioactive constituents of Radix Astragali(calycosin-7-O-ß-d-glucoside, calycosin, ononin and formononetin) after oral administration of extract of Radix Astragali (ERA)in normal and gastric ulcer mice. The abundance of Radix Astragali constituents was determined using an ultra-pressure liquid chromatograph with a photodiode array detector (UPLC-PDA), after which histograms were drawn. In comparison with normal mice, the contents of calycosin- 7-O-ß-d-glucoside, calycosin, ononin and formononetin in the stomach tissue samples of gastric ulcer mice showed significant differences at the selected time points (P < 0.05).The abundance of each of the four tested constituents in the normal groups was higher than that of the gastric ulcer groups. This study provides an empirical foundation for future studies focused on developing clinical applications of Radix Astragali
Subject(s)
Animals , Male , Female , Mice , Stomach/drug effects , Stomach Ulcer/pathology , Tissues/drug effects , Tissue Distribution , Astragalus Plant/adverse effects , Plants, Medicinal , Administration, OralABSTRACT
O presente estudo foi desenvolvido com o objetivo de avaliar o estresse oxidativo ou lipoperoxidaçäo presente na cirrose hepática e compará-lo com o existente no tecido hepático normal, assim como, avaliar os efeitos da colchicina sobre este em ambos os grupos. Foram utilizados ratos Wistar adultos, nos quais induziu-se cirrose hepática com a administraçäo de 25 doses (0,5 ml) de tetracloreto de carbono diluído em óleo mineral (1:7) e ao grupo controle foi administrado apenas óleo mineral. Após o período de induçäo da cirrose hepática, seguiu-se a administraçäo diária de colchicina (10 mg/100 g) por 90 dias e soluçäo fisiológica (1 ml/kg) como controle. A lipoperoxidaçäo foi determinada através dos métodos de substâncias reativas ao ácido tiobarbitúrico e quimiluminescência iniciada pelo hidroperóxido de tert-butil. O fígado foi submetido a avaliaçäo histológica para comprovar a presença de cirrose hepática. Os resultados obtidos demonstraram maiores níveis de lipoperoxidaçäo no grupo cirrótico quando comparado com o grupo controle (P <0,05). Este aumento na lipoperoxidaçäo foi reduzido pelo tratamento com colchicina nos animais cirróticos, näo produzindo alteraçöes nos níveis de lipoperoxidaçäo dos animais controle (P <0,05). Com base nos resultados pode-se concluir que na cirrose hepática ocorre um aumento do estresse oxidativo, se comparado ao fígado normal, sendo este passível de reduçäo pelo tratamento com colchicina.
Subject(s)
Animals , Rats , Colchicine/pharmacology , Liver Cirrhosis/physiopathology , Liver/pathology , Oxidative Stress/drug effects , Tissues/drug effects , Free Radicals , Lipid Peroxidation , Liver Cirrhosis/metabolism , Rats, Wistar , Tissues/pathologyABSTRACT
The value of cephalexin cream [1% and 2%] as a local measure in prevention of wound sepsis and promotion of healing was assessed histologically in an electrothermal burn. Electrical burn wounds were induced in guinea pigs using a soldering gun in order to initiate a full -thickness burn. These wounds were treated with cephalexin cream for twenty days. The wounds were described microscopically and studied microscopically on two sessions of ten days interval from the antibiotic application. The data obtained showed that cephalexin cream can modify healing of burnt tissue by shortening the time required for healing and almost preventing wound sepsis, by the production of granulation tissue with less pronounced acute inflammatory reaction and by the formation of small and less numerous blood vessels with rapid increase in collagen deposition