ABSTRACT
Wound age provides valuable information for the reconstruction of crime scenes and determination of the cause of death. As skin covers the outersurface of the body, it is the most vulnerable part, and dermal wound age is a critical issue in routine forensic autopsies. The aim of this study was to determine the wound age by the use of immunohistochemical study of transforming growth factors [TFG-a and TGF-pl] on human skin wounds. Samples were collected from human skin wounds after operative incisions [from a few minutes to 6 weeks] and investigated using immunohistochemistry. TGF-a was started to increase after a wound age of approximately l0min. The maximum level was between 30-60min then decreased significantly. TGF-pl was also markedly increased within 60min and remained detectable in elevated levels in older wounds [6 weeks]. Thus, it appears that TGF-a and TGF-pl can efficiently contribute to the estimation of wound age based on the evaluation of their expressions. In particular, this applies to TGF-pl because it remains in high level for long time post injury
Subject(s)
Humans , Male , Female , Transforming Growth Factor alpha/adverse effects , Transforming Growth Factor beta/adverse effects , Immunohistochemistry , Wound HealingABSTRACT
A radiação ionizante é uma das terapias utilizadas para o tratamento de neoplasias malignas de cabeça e pescoço. Todavia, pode causar complicações agudas, como mucosite, e complicações crônicas, como fibrose tecidual. A radioterapia promove aumento na deposição de colágeno e incrementa a expressão e ativação precoce do fator de crescimento transformador beta, que pode culminar nessa fibrose. O fator de crescimento transformador - beta é uma citocina multifuncional, excretada sob forma latente, com importante função na modulação do sistema fibroblasto-fibrócito, estimulando a síntese e depósito de colágeno, atuando no reparo tecidual, e diminuindo a sua degradação, especialmente em tecido irradiado. A presente revisão de literatura tem como objetivo esclarecer a relação da expressão do fator de crescimento transformador - beta em tecidos submetidos à radiação ionizante utilizada para tratamento antineoplásico, em especial na mucosa oral e reparo tecidual em lesões do tipo mucosite, realizando uma análise crítica dos trabalhos avaliados.
Ionizing radiation is a form of therapy used for the treatment of malignancies of head and neck. However, it can cause acute complications such as mucositis, and chronic complications, such as tissue fibrosis. Radiation promotes increase in collagen deposition and increases the expression and activation of the Transforming Growth Factor - beta, which may result in this fibrosis. The transforming growth factor - beta is a multifunctional cytokine, excreted latent, with an important role in the modulation of the fibroblasts- fibrocytes, stimulating synthesis and deposition of collagen, acting on tissue repair and decreasing its degradation, especially in tissue irradiated. This literature review aims to clarify the relationship between the expression of transforming growth factor - beta in tissues submitted to ionizing radiation used for anticancer treatment, particularly in the oral mucosa and wound repair in lesions with mucositis, performing a critical analyses of the assessed work.