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1.
Mem. Inst. Oswaldo Cruz ; 106(6): 769-772, Sept. 2011. ilus, tab
Article in English | LILACS | ID: lil-602064

ABSTRACT

The cave organ is a sensory receptor in the antenna pedicel of some Reduviidae. This paper describes this organ for the first time in three species of the genus Belminus, Belminus corredori, Belminus ferroae and Belminus herreri, by optical and scanning electron microscopy. The structures presented a general pattern similar to one reported for other species of Triatominae.


Subject(s)
Animals , Arthropod Antennae/ultrastructure , Insect Vectors/ultrastructure , Sensory Receptor Cells/ultrastructure , Triatominae/ultrastructure , Arthropod Antennae/cytology , Insect Vectors/classification , Microscopy, Electron, Scanning , Triatominae/classification
2.
Mem. Inst. Oswaldo Cruz ; 102(1): 13-20, Feb. 2007. tab, graf, ilus
Article in Portuguese | LILACS | ID: lil-440624

ABSTRACT

The objective of this work was to analyze the morphological, morphometrical, and histological characteristics of eggs of four triatomine bugs species still not studied, in order to understand phylogenetic aspects and to facilitate parameters used in taxonomy, with the purpose of a specific and generic characterization of these vectors in public health. The eggs of each species had come from the collections of the Laboratory of Triatomine bugs and Culicid mosquitoes of the Faculdade de Saúde Pública - USP (Faculty of Public Health/USP). The morphologic studies were carried out through optic microscopy (OM) and scanning electronic microscopy (SEM). The eggs were measured with the help of the digital paquimeter Starrett® 727. In relation to histological measures, Microtome Leica RM 2145® was used. Similarities were evidenced in the exocorial architecture of the eggs when they were studied by OM and SEM. This similarity among the eggs suggests a recent speciation, probably derived from common ancestry, representing a monophyletic group. Some structures were also detected by the histological cuts. The study has contributed to the magnification and recognition in generic and specific terms of the Triatominae subfamily. These new data will be able to subsidize a better understanding to determine roles for each vector species and to facilitate parameters to be used in taxonomy.


Subject(s)
Animals , Insect Vectors/classification , Ovum/ultrastructure , Triatominae/classification , Chagas Disease , Insect Vectors/ultrastructure , Microscopy, Electron, Scanning , Triatominae/ultrastructure
3.
Säo Paulo; s.n; 2003. [137] p. ilus, tab, graf.
Thesis in Portuguese | LILACS | ID: lil-347103

ABSTRACT

Objetivo. Analisar as características morfológicas, morfométricas e histológicas de ovos de dez espécies de triatomíneos ainda näo estudadas, visando melhor compreender aspectos filogenéticos e facilitar parâmetros usados em taxonomia, com a finalidade de uma caracterizaçäo específica e genérica desses vetores de Trypanosoma cruzi. Métodos. A investigaçäo constou de pesquisa bibliográfica, seguida de pesquisa morfológica, morfométrica e histológica de ovos de espécies provenientes do acervo do Laboratório de Triatomíneos e Culicídeos da Faculdade de Saúde Pública - USP, do Insetário do Serviço Especial de Saúde de Araraquara / SESA e do insetário de triatomíneos do Laboratório Nacional e Internacional de Referência em taxonomia de triatomíneos da FIOCRUZ/RJ. Os estudos morfológicos foram realizados por meio de Microscopia Optica e Microscopia Eletrônica de Varredura; as análises estatísticas foram estabelecidas por Análise de Variância e teste de Tukey. Na histologia utilizou-se micrótomo Leica RM 2145®. Resultados. Das dez espécies estudadas, foi possível visualizar diferenças morfológicas significantes em seis delas. No entanto, em quatro espécies houve grandes semelhanças que näo oferecem condiçöes para distinçäo das mesmas. A análise estatística revelou diferenças significantes (p<0,001, pelo teste F) entre as médias de comprimento e largura. Algumas estruturas também foram detectadas pelos cortes histológicos. Conclusöes. O estudo contribuiu para a ampliaçäo do reconhecimento genérico e específico da subfamília Triatominae. As características identificadas no exocório das cascas dos ovos podem auxiliar na separaçäo de espécies próximas. Esses novos parâmetros poderäo subsidiar a elaboraçäo de futuras chaves dicotômicas, auxiliando na determinaçäo de cada espécie vetora, e ampliando o conceito específico.


Subject(s)
Animals , Triatominae/classification , Triatominae/ultrastructure , Trypanosoma cruzi , Chagas Disease , Disease Vectors , Microscopy, Electron, Scanning
4.
Rev. ciênc. farm ; 18(2): 249-70, 1997. ilus, tab
Article in Portuguese | LILACS | ID: lil-227846

ABSTRACT

Descreveu-se, com o auxílio da microscopia óptica, os segmentos abdominais dorsais e ventrais de ninfas de 5§ estádio de Panstrongylus megistus, Rhodnius neglectus, Triatoma brasiliensis, T. infestans, T. matogrossensis e T. tibiamaculata. As estruturas estudadas foram: conexivo, áreas glabras, áreas brilhantes, placa quitinosa, estigmas, respiratórios e tubérculos dorsais.


Subject(s)
Animals , Abdomen/anatomy & histology , Triatominae/ultrastructure , Microscopy , Nymph/growth & development , Nymph/ultrastructure , Triatominae/growth & development
6.
Rev. bras. biol ; 53(2): 269-83, maio 1993. ilus, tab
Article in Portuguese | LILACS | ID: lil-129385

ABSTRACT

A fim de proporcionar novos parâmetros a serem usados na Taxonomia dos Triatomíneos, vetores da Doença de Chagas e ampliar os conhecimentos a respeito desta espécie, realizou-se um estudo da estrutura de ovos e da morfologia externa dos cinco estádios ninfais, através de microscopia óptica e eletrônica de varredura. Os ovos mostram em microscopia óptica (M.O.), a superfície do exocório do corpo e opérculo formada por áreas poligonais, mais nítidas quando observadas em microscopia eletrônica de varredura (MEV). Os 5 estádios ninfais apresentam no ápice do 3§ segmento do rostro 1+1 estruturas alongadas, que se exteriorizam; anida no 2§ segmento tarsal, um adensado apical de cerdas longas e delicadas, de coloraçäo dourada (Lent and Wygodzinsky, 1979)


Subject(s)
Animals , Nymph/ultrastructure , Ovum/ultrastructure , Triatominae/ultrastructure , Microscopy, Electron, Scanning , Nymph/growth & development , Ovum/growth & development , Triatominae/growth & development
7.
Mem. Inst. Oswaldo Cruz ; 86(1): 1-10, jan.-mar. 1991. ilus
Article in English | LILACS | ID: lil-109256

ABSTRACT

The objective of this work was to characterize, and compare different morphological types of hemocytes of Rhodnius prolixus, Rhodnius, Rhodnius neglectus, Triatoma infestans, Panstrongylus megistus, and Dipetalogaster maximus. This information provides the basis for studying the cellular immune systems of these insects. Seven morphological hemocyte types wereidentified by phase-contrast microscopy: prohemocytes, plasmatocytes, granular cells, cytocytes, oenocytoids, adipohemocytes and giant cells. All seven types of hemocytes are not present in every species. For example, adipohemocytes and oenocytoids were not observed in P. megistus and P. infestans, and giant cells were rarely found in any of the species studied. The hemocytes of rhodnius and Dipetalogaster are more similar to each other than those from Triatoma and Panstrongylus which in turn closely resemble each other. Emphasis is placed on methodological problems arising in this work wicah are discussed in detail


Subject(s)
Animals , Male , Female , Hemocytes/ultrastructure , Triatominae/ultrastructure , Hemolymph/cytology , Microscopy, Phase-Contrast
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